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Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus

The mRNA expression of marker genes for bone formation. The mRNAs of Collagen type I and OCN from heat-, and ibanronate-treated rats and control, respectively were extracted and measured by RT-PCR (A). The histogram depicts the mRNA of Collagen type I and osteocalcin (OCN) compared to β-actin (B). Asterisk (*) indicates a significant difference (p<0.05) compared with the control (#). Each column represents the mean ± S.E.M. of three or four separate experiments.
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f6-bt-22-563: The mRNA expression of marker genes for bone formation. The mRNAs of Collagen type I and OCN from heat-, and ibanronate-treated rats and control, respectively were extracted and measured by RT-PCR (A). The histogram depicts the mRNA of Collagen type I and osteocalcin (OCN) compared to β-actin (B). Asterisk (*) indicates a significant difference (p<0.05) compared with the control (#). Each column represents the mean ± S.E.M. of three or four separate experiments.

Mentions: Several transcription factors and genes play a vital role for bone formation. In our study we tried to see the mRNA expression of Collagen type I and osteocalcin (OCN) in the tissue around the implant. To do so we have extracted the tissue around the implant after four week of implantation. The mRNA expression of Collagen type I was down regulated while the expression of OCN remained unchanged. Expression of these genes was analyzed by RT-PCR in both control and ibandronate coated titanium alloy (Fig. 6).


Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

The mRNA expression of marker genes for bone formation. The mRNAs of Collagen type I and OCN from heat-, and ibanronate-treated rats and control, respectively were extracted and measured by RT-PCR (A). The histogram depicts the mRNA of Collagen type I and osteocalcin (OCN) compared to β-actin (B). Asterisk (*) indicates a significant difference (p<0.05) compared with the control (#). Each column represents the mean ± S.E.M. of three or four separate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256038&req=5

f6-bt-22-563: The mRNA expression of marker genes for bone formation. The mRNAs of Collagen type I and OCN from heat-, and ibanronate-treated rats and control, respectively were extracted and measured by RT-PCR (A). The histogram depicts the mRNA of Collagen type I and osteocalcin (OCN) compared to β-actin (B). Asterisk (*) indicates a significant difference (p<0.05) compared with the control (#). Each column represents the mean ± S.E.M. of three or four separate experiments.
Mentions: Several transcription factors and genes play a vital role for bone formation. In our study we tried to see the mRNA expression of Collagen type I and osteocalcin (OCN) in the tissue around the implant. To do so we have extracted the tissue around the implant after four week of implantation. The mRNA expression of Collagen type I was down regulated while the expression of OCN remained unchanged. Expression of these genes was analyzed by RT-PCR in both control and ibandronate coated titanium alloy (Fig. 6).

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus