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Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus

Microstructure parameters measured by μCT. Appearance of the implants by μCT scanner (A). Bone volume/tissue volume, trabecular bone/trabecular thickness, trabecular separation and a trabecular number for one and four weeks, respectively (B). Data are expressed as mean± S.E.M. *p<0.05 vs. control (#).
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f3-bt-22-563: Microstructure parameters measured by μCT. Appearance of the implants by μCT scanner (A). Bone volume/tissue volume, trabecular bone/trabecular thickness, trabecular separation and a trabecular number for one and four weeks, respectively (B). Data are expressed as mean± S.E.M. *p<0.05 vs. control (#).

Mentions: μCT analysis of the sample revealed effects in various microstructure parameters, such as BV/TV, Tb/Th, Tb/Sp, and Tb/N, over periods of one and four weeks after implantation (Fig. 3A). Our quantitative evaluation provided more detailed information on osseointegration and trabecular parameters around the screws. Compared with controls, the treatment combination that included ibandronate significantly improved the trabecular bone/trabecular thickness (Tb/Th) in 1 week and trabecular number (Tb N) in 4 weeks, while a significant decrease in trabecular separation (Tb.Sp) in 4 weeks was observed (Fig. 3B). Most changes in microstructure parameters were statistically significant when compared with the control alloy, which was treated with heat alone. This demonstrates that bisphosphonate coating and heat treatment of implants improves microstructure parameters, which provides evidence of ongoing osseointegration.


Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Microstructure parameters measured by μCT. Appearance of the implants by μCT scanner (A). Bone volume/tissue volume, trabecular bone/trabecular thickness, trabecular separation and a trabecular number for one and four weeks, respectively (B). Data are expressed as mean± S.E.M. *p<0.05 vs. control (#).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256038&req=5

f3-bt-22-563: Microstructure parameters measured by μCT. Appearance of the implants by μCT scanner (A). Bone volume/tissue volume, trabecular bone/trabecular thickness, trabecular separation and a trabecular number for one and four weeks, respectively (B). Data are expressed as mean± S.E.M. *p<0.05 vs. control (#).
Mentions: μCT analysis of the sample revealed effects in various microstructure parameters, such as BV/TV, Tb/Th, Tb/Sp, and Tb/N, over periods of one and four weeks after implantation (Fig. 3A). Our quantitative evaluation provided more detailed information on osseointegration and trabecular parameters around the screws. Compared with controls, the treatment combination that included ibandronate significantly improved the trabecular bone/trabecular thickness (Tb/Th) in 1 week and trabecular number (Tb N) in 4 weeks, while a significant decrease in trabecular separation (Tb.Sp) in 4 weeks was observed (Fig. 3B). Most changes in microstructure parameters were statistically significant when compared with the control alloy, which was treated with heat alone. This demonstrates that bisphosphonate coating and heat treatment of implants improves microstructure parameters, which provides evidence of ongoing osseointegration.

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus