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Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus

Histomorphometric analysis of the bone area and percentage bone contact. The means of 50 individual readings (10×5 slides) were calculated. The bone contact (A) is defined as the direct bone implant interface to the total implant surface, and the bone area (B) is defined as the ratio of newly formed bone area to the whole area within the figure. Data were expressed as mean ± S.E.M. *p<0.05 vs. the control group.
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f2-bt-22-563: Histomorphometric analysis of the bone area and percentage bone contact. The means of 50 individual readings (10×5 slides) were calculated. The bone contact (A) is defined as the direct bone implant interface to the total implant surface, and the bone area (B) is defined as the ratio of newly formed bone area to the whole area within the figure. Data were expressed as mean ± S.E.M. *p<0.05 vs. the control group.

Mentions: The bone responses to control and ibandronate-coated implants were significantly different. Four weeks following implantation, 324.205 μm2 of bone area (BA) and 29.330 μm of bone contact (BC) surface were observed in the control group, whereas these same measurements were 479.721 μm2 and 53.51 μm, respectively, in the ibandronate and heat-treated implants (Fig. 2A, B) The implants treated with ibandronate showed a significantly higher bone contact ratio (p<0.05) compared to the control implants. Additionally, the relative bone area was also found to be significantly higher than in the controls. These significant increases in both bone contact and bone area suggest that ibandronate coating is a promising method for enhancing the biocompatibility of implant materials.


Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Histomorphometric analysis of the bone area and percentage bone contact. The means of 50 individual readings (10×5 slides) were calculated. The bone contact (A) is defined as the direct bone implant interface to the total implant surface, and the bone area (B) is defined as the ratio of newly formed bone area to the whole area within the figure. Data were expressed as mean ± S.E.M. *p<0.05 vs. the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256038&req=5

f2-bt-22-563: Histomorphometric analysis of the bone area and percentage bone contact. The means of 50 individual readings (10×5 slides) were calculated. The bone contact (A) is defined as the direct bone implant interface to the total implant surface, and the bone area (B) is defined as the ratio of newly formed bone area to the whole area within the figure. Data were expressed as mean ± S.E.M. *p<0.05 vs. the control group.
Mentions: The bone responses to control and ibandronate-coated implants were significantly different. Four weeks following implantation, 324.205 μm2 of bone area (BA) and 29.330 μm of bone contact (BC) surface were observed in the control group, whereas these same measurements were 479.721 μm2 and 53.51 μm, respectively, in the ibandronate and heat-treated implants (Fig. 2A, B) The implants treated with ibandronate showed a significantly higher bone contact ratio (p<0.05) compared to the control implants. Additionally, the relative bone area was also found to be significantly higher than in the controls. These significant increases in both bone contact and bone area suggest that ibandronate coating is a promising method for enhancing the biocompatibility of implant materials.

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus