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Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus

Photographs of bone after removing an implant. The bone was fixed according to the shape of the implant (A). Histological appearance of bone after staining with hematoxylin and eosin (B). The picture depicts the ongoing fixation of the bone to the implant. Photographs captured at 50X, and the scale bar is 50 μM.
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f1-bt-22-563: Photographs of bone after removing an implant. The bone was fixed according to the shape of the implant (A). Histological appearance of bone after staining with hematoxylin and eosin (B). The picture depicts the ongoing fixation of the bone to the implant. Photographs captured at 50X, and the scale bar is 50 μM.

Mentions: To analyze the effect of ibandronate-treated alloy on bone, we evaluated new bone formation around tibial implants after four weeks. The rats were implanted with either an untreated alloy, which was used as a positive control, or ibandronate and a heat-treated alloy placed between the tibia and the femur of the male rats for four weeks, which was our experimental condition (Fig. 1A). Previous studies have established that BP has the ability to promote osteoblast formation in bone and can also enhance osseointegration. As expected, four weeks after implantation, circumferential bone tissue formation was detectable on both the implants and the bone that was in close contact with the implant on both the untreated as well as the ibandronate-coated implants. Furthermore, healing and hard tissue integration of the implants was achieved over a four-week period, and no signs of inflammation were detected in any of the specimens.


Evaluation of Osseointegration around Tibial Implants in Rats by Ibandronate-Treated Nanotubular Ti-32Nb-5Zr Alloy.

Nepal M, Li L, Bae TS, Kim BI, Soh Y - Biomol Ther (Seoul) (2014)

Photographs of bone after removing an implant. The bone was fixed according to the shape of the implant (A). Histological appearance of bone after staining with hematoxylin and eosin (B). The picture depicts the ongoing fixation of the bone to the implant. Photographs captured at 50X, and the scale bar is 50 μM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256038&req=5

f1-bt-22-563: Photographs of bone after removing an implant. The bone was fixed according to the shape of the implant (A). Histological appearance of bone after staining with hematoxylin and eosin (B). The picture depicts the ongoing fixation of the bone to the implant. Photographs captured at 50X, and the scale bar is 50 μM.
Mentions: To analyze the effect of ibandronate-treated alloy on bone, we evaluated new bone formation around tibial implants after four weeks. The rats were implanted with either an untreated alloy, which was used as a positive control, or ibandronate and a heat-treated alloy placed between the tibia and the femur of the male rats for four weeks, which was our experimental condition (Fig. 1A). Previous studies have established that BP has the ability to promote osteoblast formation in bone and can also enhance osseointegration. As expected, four weeks after implantation, circumferential bone tissue formation was detectable on both the implants and the bone that was in close contact with the implant on both the untreated as well as the ibandronate-coated implants. Furthermore, healing and hard tissue integration of the implants was achieved over a four-week period, and no signs of inflammation were detected in any of the specimens.

Bottom Line: Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy.Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment.Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project.

ABSTRACT
Materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. This study was designed to evaluate bone response to titanium alloy containing Ti-32Nb-5Zr with nanostructure, anodic oxidation, heat treatment, and ibandronate coating. Rats were randomly assigned to two groups for implantation of titanium alloy (untreated) as the control group and titanium alloy group coated with ibandronate as the experimental group. Then, the implants were inserted in both tibiae of the rats for four weeks. After implantation, bone implant interface, trabecular microstructure, mechanical fixation was evaluated by histology, micro-computed tomography (μCT) and the push-out test, respectively. We found that the anodized, heat-treated and ibandronate-coated titanium alloy triggered pronounced bone implant integration and early bone formation. Ibandronate-coated implants showed elevated values for removal torque and a higher level of BV/TV, trabecular thickness and separation upon analysis with μCT and mechanical testing. Similarly, higher bone contact and a larger percentage bone area were observed via histology compared to untreated alloy. Furthermore, well coating of ibandronate with alloy was observed by vitro releasing experiment. Our study provided evidences that the coating of bisphosphonate onto the anodized and heat-treated nanostructure of titanium alloy had a positive effect on implant fixation.

No MeSH data available.


Related in: MedlinePlus