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Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells.

Wang Z, Wang D, Li Y, Zhang X - Biomol Ther (Seoul) (2014)

Bottom Line: The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil.Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content.Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, HE's University, Shenyang, Liaoning, 110163, P.R.China.

ABSTRACT
Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

No MeSH data available.


Related in: MedlinePlus

Effects of verapamil on H2O2-induced mRNA expression of Caspase-3 in HLEC. DNA strips show expression levels of Caspase-3 on agarose gel electrophoresis. ###p<0.01 versus control, **p<0.01 versus H2O2.
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f4-bt-22-553: Effects of verapamil on H2O2-induced mRNA expression of Caspase-3 in HLEC. DNA strips show expression levels of Caspase-3 on agarose gel electrophoresis. ###p<0.01 versus control, **p<0.01 versus H2O2.

Mentions: As shown in Fig. 4, mRNA expression of caspase-3 was significantly upregulated when treated with H2O2 compared with control group. While in the verapamil group, Caspase-3 mRNA expression was significantly lower than in the H2O2 group.


Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells.

Wang Z, Wang D, Li Y, Zhang X - Biomol Ther (Seoul) (2014)

Effects of verapamil on H2O2-induced mRNA expression of Caspase-3 in HLEC. DNA strips show expression levels of Caspase-3 on agarose gel electrophoresis. ###p<0.01 versus control, **p<0.01 versus H2O2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256036&req=5

f4-bt-22-553: Effects of verapamil on H2O2-induced mRNA expression of Caspase-3 in HLEC. DNA strips show expression levels of Caspase-3 on agarose gel electrophoresis. ###p<0.01 versus control, **p<0.01 versus H2O2.
Mentions: As shown in Fig. 4, mRNA expression of caspase-3 was significantly upregulated when treated with H2O2 compared with control group. While in the verapamil group, Caspase-3 mRNA expression was significantly lower than in the H2O2 group.

Bottom Line: The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil.Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content.Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, HE's University, Shenyang, Liaoning, 110163, P.R.China.

ABSTRACT
Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

No MeSH data available.


Related in: MedlinePlus