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Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of R. fasciculatum. The retention time of quercitrin was specified on the chromatogram for the comparison. The mobile phase was 0.1% aqueous acetic acid (A) and acetonitrile (B) with a gradient program as follows: 0–5 min; isocratic 95% A; 5–60 min, linear gradient elution, 95-0% A; at a flow rate of 1 mL/min. The sample injection volume was 20 μL and the peak was monitored at a wavelength of 360 nm.
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f6-bt-22-547: HPLC chromatogram of R. fasciculatum. The retention time of quercitrin was specified on the chromatogram for the comparison. The mobile phase was 0.1% aqueous acetic acid (A) and acetonitrile (B) with a gradient program as follows: 0–5 min; isocratic 95% A; 5–60 min, linear gradient elution, 95-0% A; at a flow rate of 1 mL/min. The sample injection volume was 20 μL and the peak was monitored at a wavelength of 360 nm.

Mentions: To confirm the constituents of R. fasciculatum, an HPLC analysis was performed. HPLC measurement of R. fasciculatum demonstrated various chromatographic peaks. Comparing the chromatographic peaks of R. fasciculatum with reference chromatographic peaks, quercitrin was identified (Fig. 6).


Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

HPLC chromatogram of R. fasciculatum. The retention time of quercitrin was specified on the chromatogram for the comparison. The mobile phase was 0.1% aqueous acetic acid (A) and acetonitrile (B) with a gradient program as follows: 0–5 min; isocratic 95% A; 5–60 min, linear gradient elution, 95-0% A; at a flow rate of 1 mL/min. The sample injection volume was 20 μL and the peak was monitored at a wavelength of 360 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256035&req=5

f6-bt-22-547: HPLC chromatogram of R. fasciculatum. The retention time of quercitrin was specified on the chromatogram for the comparison. The mobile phase was 0.1% aqueous acetic acid (A) and acetonitrile (B) with a gradient program as follows: 0–5 min; isocratic 95% A; 5–60 min, linear gradient elution, 95-0% A; at a flow rate of 1 mL/min. The sample injection volume was 20 μL and the peak was monitored at a wavelength of 360 nm.
Mentions: To confirm the constituents of R. fasciculatum, an HPLC analysis was performed. HPLC measurement of R. fasciculatum demonstrated various chromatographic peaks. Comparing the chromatographic peaks of R. fasciculatum with reference chromatographic peaks, quercitrin was identified (Fig. 6).

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus