Limits...
Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of R. fasciculatum on the NF-κB activation in the nuclei of LPS-stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.5 mg/ml) for 1 h and then stimulated with LPS for 2 h. (A) Nuclear extracts were prepared as described in the Materials and Methods section and evaluated for RelA/p65 via Western blot analysis. (B) The relative levels of NF-κB were represented. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4256035&req=5

f5-bt-22-547: Effect of R. fasciculatum on the NF-κB activation in the nuclei of LPS-stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.5 mg/ml) for 1 h and then stimulated with LPS for 2 h. (A) Nuclear extracts were prepared as described in the Materials and Methods section and evaluated for RelA/p65 via Western blot analysis. (B) The relative levels of NF-κB were represented. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).

Mentions: As the suppression of NF-κB activation has been linked with anti-inflammation, we speculated that the effects of R. fasciculatum might be mediated, at least in part, via the suppression of NF-κB activation. Additionally, because NF-κB activation requires the nuclear translocation of the RelA/p65 subunit of NF-κB, we evaluated the effects of R. fasciculatum on the nuclear pool of RelA/p65 protein via western blot analysis. In LPS-stimulated cells, the levels of Rel/p65 were increased, but R. fasciculatum reduced these enhanced nuclear levels of Rel/p65 (Fig. 5A). The relative levels of NF-κB (in nucleus) were represented in Fig. 5B.


Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Effect of R. fasciculatum on the NF-κB activation in the nuclei of LPS-stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.5 mg/ml) for 1 h and then stimulated with LPS for 2 h. (A) Nuclear extracts were prepared as described in the Materials and Methods section and evaluated for RelA/p65 via Western blot analysis. (B) The relative levels of NF-κB were represented. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256035&req=5

f5-bt-22-547: Effect of R. fasciculatum on the NF-κB activation in the nuclei of LPS-stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.5 mg/ml) for 1 h and then stimulated with LPS for 2 h. (A) Nuclear extracts were prepared as described in the Materials and Methods section and evaluated for RelA/p65 via Western blot analysis. (B) The relative levels of NF-κB were represented. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
Mentions: As the suppression of NF-κB activation has been linked with anti-inflammation, we speculated that the effects of R. fasciculatum might be mediated, at least in part, via the suppression of NF-κB activation. Additionally, because NF-κB activation requires the nuclear translocation of the RelA/p65 subunit of NF-κB, we evaluated the effects of R. fasciculatum on the nuclear pool of RelA/p65 protein via western blot analysis. In LPS-stimulated cells, the levels of Rel/p65 were increased, but R. fasciculatum reduced these enhanced nuclear levels of Rel/p65 (Fig. 5A). The relative levels of NF-κB (in nucleus) were represented in Fig. 5B.

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus