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Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

Effects of R. fasciculatum on the production of inflammatory cytokines in LPS -stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.1–0.5 mg/ml) for 1 h and then stimulated LPS (1 μg/ml) for 12 h. The levels of inflammatory cytokines (TNF-α and IL-6) were measured from cell supernatant using ELISA. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
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f4-bt-22-547: Effects of R. fasciculatum on the production of inflammatory cytokines in LPS -stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.1–0.5 mg/ml) for 1 h and then stimulated LPS (1 μg/ml) for 12 h. The levels of inflammatory cytokines (TNF-α and IL-6) were measured from cell supernatant using ELISA. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).

Mentions: In an effort to determine the molecular mechanism of R. fasciculatum, the macrophage cell line, Raw 264.7, was employed in this study. We determined whether R. fasciculatum modulates the LPS-induced production of TNF-α and IL-6. The levels of TNF-α and IL-6 in culture supernatants were measured via ELISA. As is shown in Fig. 4, the production of TNF-α and IL-6 in response to LPS was inhibited as the result of pre-treatment with R. fasciculatum in a dose-dependent manner. The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. Additionally, we observed that R. fasciculatum did not affect cell viability (data not shown).


Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Effects of R. fasciculatum on the production of inflammatory cytokines in LPS -stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.1–0.5 mg/ml) for 1 h and then stimulated LPS (1 μg/ml) for 12 h. The levels of inflammatory cytokines (TNF-α and IL-6) were measured from cell supernatant using ELISA. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256035&req=5

f4-bt-22-547: Effects of R. fasciculatum on the production of inflammatory cytokines in LPS -stimulated Raw 264.7 cells. Cells were pre-treated with R. fasciculatum (0.1–0.5 mg/ml) for 1 h and then stimulated LPS (1 μg/ml) for 12 h. The levels of inflammatory cytokines (TNF-α and IL-6) were measured from cell supernatant using ELISA. Statistical evaluation of the results was performed by independent t-test. All data were represented in the mean ± S.D. of triplicate determinations from triplicate separate experiments (#p<0.05 vs. control, *p<0.05 vs. LPS alone).
Mentions: In an effort to determine the molecular mechanism of R. fasciculatum, the macrophage cell line, Raw 264.7, was employed in this study. We determined whether R. fasciculatum modulates the LPS-induced production of TNF-α and IL-6. The levels of TNF-α and IL-6 in culture supernatants were measured via ELISA. As is shown in Fig. 4, the production of TNF-α and IL-6 in response to LPS was inhibited as the result of pre-treatment with R. fasciculatum in a dose-dependent manner. The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. Additionally, we observed that R. fasciculatum did not affect cell viability (data not shown).

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus