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Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of R. fasciculatum on the DNCB-induced dermatitis and serum IgE level. (A) The BALB/c mice (n=6) were sensitized with 100 μl of 0.1% DNCB in acetone-olive oil (3:1) or vehicle (acetone/olive oil=3:1) applied to the dorsal skin twice each week for a total period of 5 weeks. After 3 weeks, R. fasciculatum (200 mg/kg) was orally administered 2 week prior to the end of the experiment. (B) Blood samples were collected and then levels of serum IgE in the indicated groups were measured using ELISA method. Statistical evaluation of the results was performed by independent t-test. Each datum represents the means ± S.D. of three independent experiments (#p<0.05 vs. control group, *p<0.05 vs. DNCB -treated group).
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f3-bt-22-547: Effect of R. fasciculatum on the DNCB-induced dermatitis and serum IgE level. (A) The BALB/c mice (n=6) were sensitized with 100 μl of 0.1% DNCB in acetone-olive oil (3:1) or vehicle (acetone/olive oil=3:1) applied to the dorsal skin twice each week for a total period of 5 weeks. After 3 weeks, R. fasciculatum (200 mg/kg) was orally administered 2 week prior to the end of the experiment. (B) Blood samples were collected and then levels of serum IgE in the indicated groups were measured using ELISA method. Statistical evaluation of the results was performed by independent t-test. Each datum represents the means ± S.D. of three independent experiments (#p<0.05 vs. control group, *p<0.05 vs. DNCB -treated group).

Mentions: In order to evaluate the regulatory effects of R. fasciculatum in an atopic dermatitis in vivo model, DNCB was administered to BALB/c mice. As shown in Fig. 3A, when mice were treated for 2 week with R. fasciculatum, the atopic dermatitis was recovered to a significant extent. To evaluate the effects of R. fasciculatum on IgE levels in serum, blood samples were collected. The levels of IgE were measured via ELISA. The results showed that IgE levels were increased as the result of DNCB exposure, but this phenomenon was significantly reduced in the R. fasciculatum group (Fig. 3B).


Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Effect of R. fasciculatum on the DNCB-induced dermatitis and serum IgE level. (A) The BALB/c mice (n=6) were sensitized with 100 μl of 0.1% DNCB in acetone-olive oil (3:1) or vehicle (acetone/olive oil=3:1) applied to the dorsal skin twice each week for a total period of 5 weeks. After 3 weeks, R. fasciculatum (200 mg/kg) was orally administered 2 week prior to the end of the experiment. (B) Blood samples were collected and then levels of serum IgE in the indicated groups were measured using ELISA method. Statistical evaluation of the results was performed by independent t-test. Each datum represents the means ± S.D. of three independent experiments (#p<0.05 vs. control group, *p<0.05 vs. DNCB -treated group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256035&req=5

f3-bt-22-547: Effect of R. fasciculatum on the DNCB-induced dermatitis and serum IgE level. (A) The BALB/c mice (n=6) were sensitized with 100 μl of 0.1% DNCB in acetone-olive oil (3:1) or vehicle (acetone/olive oil=3:1) applied to the dorsal skin twice each week for a total period of 5 weeks. After 3 weeks, R. fasciculatum (200 mg/kg) was orally administered 2 week prior to the end of the experiment. (B) Blood samples were collected and then levels of serum IgE in the indicated groups were measured using ELISA method. Statistical evaluation of the results was performed by independent t-test. Each datum represents the means ± S.D. of three independent experiments (#p<0.05 vs. control group, *p<0.05 vs. DNCB -treated group).
Mentions: In order to evaluate the regulatory effects of R. fasciculatum in an atopic dermatitis in vivo model, DNCB was administered to BALB/c mice. As shown in Fig. 3A, when mice were treated for 2 week with R. fasciculatum, the atopic dermatitis was recovered to a significant extent. To evaluate the effects of R. fasciculatum on IgE levels in serum, blood samples were collected. The levels of IgE were measured via ELISA. The results showed that IgE levels were increased as the result of DNCB exposure, but this phenomenon was significantly reduced in the R. fasciculatum group (Fig. 3B).

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus