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Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of R. fasciculatum on scratching behavior in ICR mice. R. fasciculatum (200 mg/kg) was orally administered 1 h before compound 48/80 (50 μg/kg) intradermally injection. Scratching behaviors was counted as one incident of scratching for 30 min. Statistical evaluation of the results was performed by ANOVA with a Tukey post hoc test. Each datum represents the means ± S.D. of experiments (#p<0.05 vs. control group, *p<0.05 vs. compound 48/80 or histamine-treated group).
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f1-bt-22-547: Effect of R. fasciculatum on scratching behavior in ICR mice. R. fasciculatum (200 mg/kg) was orally administered 1 h before compound 48/80 (50 μg/kg) intradermally injection. Scratching behaviors was counted as one incident of scratching for 30 min. Statistical evaluation of the results was performed by ANOVA with a Tukey post hoc test. Each datum represents the means ± S.D. of experiments (#p<0.05 vs. control group, *p<0.05 vs. compound 48/80 or histamine-treated group).

Mentions: In addition, we investigate the contribution of R. fasciculatum in histamine-induced scratching behavior. As shown in Fig. 1, orally administered R. fasciculatum inhibited the scratching behaviors by 46.11%. Terfenadine was used as a positive control in this study.


Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro.

Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY - Biomol Ther (Seoul) (2014)

Effect of R. fasciculatum on scratching behavior in ICR mice. R. fasciculatum (200 mg/kg) was orally administered 1 h before compound 48/80 (50 μg/kg) intradermally injection. Scratching behaviors was counted as one incident of scratching for 30 min. Statistical evaluation of the results was performed by ANOVA with a Tukey post hoc test. Each datum represents the means ± S.D. of experiments (#p<0.05 vs. control group, *p<0.05 vs. compound 48/80 or histamine-treated group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256035&req=5

f1-bt-22-547: Effect of R. fasciculatum on scratching behavior in ICR mice. R. fasciculatum (200 mg/kg) was orally administered 1 h before compound 48/80 (50 μg/kg) intradermally injection. Scratching behaviors was counted as one incident of scratching for 30 min. Statistical evaluation of the results was performed by ANOVA with a Tukey post hoc test. Each datum represents the means ± S.D. of experiments (#p<0.05 vs. control group, *p<0.05 vs. compound 48/80 or histamine-treated group).
Mentions: In addition, we investigate the contribution of R. fasciculatum in histamine-induced scratching behavior. As shown in Fig. 1, orally administered R. fasciculatum inhibited the scratching behaviors by 46.11%. Terfenadine was used as a positive control in this study.

Bottom Line: However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood.Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells.We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Medicinal Pharmacology, College of Health and Welfare, Daegu Haany University, Gyeongsan 712-715.

ABSTRACT
Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The maximal rates of TNF-α and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus