Limits...
Novel Suppressive Effects of Ketotifen on Migration and Invasion of MDA-MB-231 and HT-1080 Cancer Cells.

Kim HJ, Park MK, Kim SY, Lee CH - Biomol Ther (Seoul) (2014)

Bottom Line: Therefore, considerable effort is being made to inhibit metastasis.The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells.Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion.

View Article: PubMed Central - PubMed

Affiliation: BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University, Seoul 100-715.

ABSTRACT
The high mortality rates associated with cancer reflect the metastatic spread of tumor cells from the site of their origin. Metastasis, in fact, is the cause of 90% of cancer deaths. Therefore, considerable effort is being made to inhibit metastasis. In the present study, we screened ketotifen for anti-migratory and anti-invasive activities against MDA-MB-231 breast cancer and HT-1080 fibrosarcoma cancer cells. Cancer cell migration and invasion were measured using multi-well chambers. Additionally, western blots were used to examine the effects of ketotifen on the expressions of CDC42, Rho, Rac, and matrix metalloproteinase 9 (MMP-9). The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells. Ketotifen also suppressed the expressions of CDC42, Rac, and Rho, which, significantly, are involved in MDA-MB-231 and HT-1080 cancer cell migration. Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion. The overall data suggested that ketotifen suppresses the migration and invasion of MDA-MB-231 and HT-1080 cancer cells via inhibition of CDC42, Rac, Rho, and MMP-9 expression.

No MeSH data available.


Related in: MedlinePlus

Ketotifen inhibited the invasion of MDA-MB-231 and HT-1080 cells. (A) Effect of ketotifen on MDA-MB-231 cell invasion. MDAMB-231 cells (5×104/well) were treated with vehicle or increasing concentration (1, 5, 10, 25 μM) of ketotifen for 24 h. Cells invaded through the membrane after 24 h of incubation. (B) Photographs of invaded MDA-MB-231 cells. (C) Effect of ketotifen on HT-1080 cell invasion. HT-1080 cells (5×104/well) were treated with vehicle or increasing concentrations (1, 5, 10, 25 μM) of ketotifen and treated with TPA (20 nM) for 24 h. Cells invaded through the membrane after 24 h of incubation were stained and photographed. (D) Photographs of invaded HT-1080 cells. For the invasion assay, the upper-chamber transwells were coated with Matrigel (500 μg/ml). After incubation, cells invading the lower surface of the membrane were fixed with methanol, stained with hematoxylin and eosin (H&E), and subsequently counted under four randomly selected high-power (400×) fields. The lower chambers contained 10% FBS except NT (not treated with 10% FBS). *p<0.05, **p<0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4256034&req=5

f3-bt-22-540: Ketotifen inhibited the invasion of MDA-MB-231 and HT-1080 cells. (A) Effect of ketotifen on MDA-MB-231 cell invasion. MDAMB-231 cells (5×104/well) were treated with vehicle or increasing concentration (1, 5, 10, 25 μM) of ketotifen for 24 h. Cells invaded through the membrane after 24 h of incubation. (B) Photographs of invaded MDA-MB-231 cells. (C) Effect of ketotifen on HT-1080 cell invasion. HT-1080 cells (5×104/well) were treated with vehicle or increasing concentrations (1, 5, 10, 25 μM) of ketotifen and treated with TPA (20 nM) for 24 h. Cells invaded through the membrane after 24 h of incubation were stained and photographed. (D) Photographs of invaded HT-1080 cells. For the invasion assay, the upper-chamber transwells were coated with Matrigel (500 μg/ml). After incubation, cells invading the lower surface of the membrane were fixed with methanol, stained with hematoxylin and eosin (H&E), and subsequently counted under four randomly selected high-power (400×) fields. The lower chambers contained 10% FBS except NT (not treated with 10% FBS). *p<0.05, **p<0.01.

Mentions: We also examined the effects of ketotifen on the invasion of the same two MDA-MB-231 and HT-1080 cancer cell lines. In the results, ketotifen dose-dependently suppressed the invasion of MDA-MB-231 cells (Fig. 3A, 3B) as well as the TPA-induced invasion of HT-1080 cells (Fig. 3C, 3D).


Novel Suppressive Effects of Ketotifen on Migration and Invasion of MDA-MB-231 and HT-1080 Cancer Cells.

Kim HJ, Park MK, Kim SY, Lee CH - Biomol Ther (Seoul) (2014)

Ketotifen inhibited the invasion of MDA-MB-231 and HT-1080 cells. (A) Effect of ketotifen on MDA-MB-231 cell invasion. MDAMB-231 cells (5×104/well) were treated with vehicle or increasing concentration (1, 5, 10, 25 μM) of ketotifen for 24 h. Cells invaded through the membrane after 24 h of incubation. (B) Photographs of invaded MDA-MB-231 cells. (C) Effect of ketotifen on HT-1080 cell invasion. HT-1080 cells (5×104/well) were treated with vehicle or increasing concentrations (1, 5, 10, 25 μM) of ketotifen and treated with TPA (20 nM) for 24 h. Cells invaded through the membrane after 24 h of incubation were stained and photographed. (D) Photographs of invaded HT-1080 cells. For the invasion assay, the upper-chamber transwells were coated with Matrigel (500 μg/ml). After incubation, cells invading the lower surface of the membrane were fixed with methanol, stained with hematoxylin and eosin (H&E), and subsequently counted under four randomly selected high-power (400×) fields. The lower chambers contained 10% FBS except NT (not treated with 10% FBS). *p<0.05, **p<0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256034&req=5

f3-bt-22-540: Ketotifen inhibited the invasion of MDA-MB-231 and HT-1080 cells. (A) Effect of ketotifen on MDA-MB-231 cell invasion. MDAMB-231 cells (5×104/well) were treated with vehicle or increasing concentration (1, 5, 10, 25 μM) of ketotifen for 24 h. Cells invaded through the membrane after 24 h of incubation. (B) Photographs of invaded MDA-MB-231 cells. (C) Effect of ketotifen on HT-1080 cell invasion. HT-1080 cells (5×104/well) were treated with vehicle or increasing concentrations (1, 5, 10, 25 μM) of ketotifen and treated with TPA (20 nM) for 24 h. Cells invaded through the membrane after 24 h of incubation were stained and photographed. (D) Photographs of invaded HT-1080 cells. For the invasion assay, the upper-chamber transwells were coated with Matrigel (500 μg/ml). After incubation, cells invading the lower surface of the membrane were fixed with methanol, stained with hematoxylin and eosin (H&E), and subsequently counted under four randomly selected high-power (400×) fields. The lower chambers contained 10% FBS except NT (not treated with 10% FBS). *p<0.05, **p<0.01.
Mentions: We also examined the effects of ketotifen on the invasion of the same two MDA-MB-231 and HT-1080 cancer cell lines. In the results, ketotifen dose-dependently suppressed the invasion of MDA-MB-231 cells (Fig. 3A, 3B) as well as the TPA-induced invasion of HT-1080 cells (Fig. 3C, 3D).

Bottom Line: Therefore, considerable effort is being made to inhibit metastasis.The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells.Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion.

View Article: PubMed Central - PubMed

Affiliation: BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University, Seoul 100-715.

ABSTRACT
The high mortality rates associated with cancer reflect the metastatic spread of tumor cells from the site of their origin. Metastasis, in fact, is the cause of 90% of cancer deaths. Therefore, considerable effort is being made to inhibit metastasis. In the present study, we screened ketotifen for anti-migratory and anti-invasive activities against MDA-MB-231 breast cancer and HT-1080 fibrosarcoma cancer cells. Cancer cell migration and invasion were measured using multi-well chambers. Additionally, western blots were used to examine the effects of ketotifen on the expressions of CDC42, Rho, Rac, and matrix metalloproteinase 9 (MMP-9). The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells. Ketotifen also suppressed the expressions of CDC42, Rac, and Rho, which, significantly, are involved in MDA-MB-231 and HT-1080 cancer cell migration. Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion. The overall data suggested that ketotifen suppresses the migration and invasion of MDA-MB-231 and HT-1080 cancer cells via inhibition of CDC42, Rac, Rho, and MMP-9 expression.

No MeSH data available.


Related in: MedlinePlus