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Non-thermal atmospheric-pressure plasma possible application in wound healing.

Haertel B, von Woedtke T, Weltmann KD, Lindequist U - Biomol Ther (Seoul) (2014)

Bottom Line: Therefore, it cannot be equated with plasma from blood; it is not biological in nature.This review emphasizes plasma effects on wound healing.We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy, Ernst-Moritz-Arndt University of Greifswald, D17489 Greifswald, Germany.

ABSTRACT
Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

No MeSH data available.


Related in: MedlinePlus

Detection of oxidized DNA bases, namely (A) 8-hydroxy-2′-deoxyguanosine (8-OHdG) and (B) N6-etheno-2-deoxyadenosine after treatment of HaCaT keratinocytes with the kINPen 09 or hydrogen peroxide (100 μM). 24 h after exposure of cells to plasma they were fixed and stained with the antibodies 2E2 for 8-OHdG for and EMA-1 for N6-etheno-2′-desoxyadenosine. Binding of antibodies was detected by using flow cytometry. Mean fluorescence intensities (MFI) are expressed as percentage of that of untreated control cells. Mean ± SEM, *p<0.05 vs. untreated control cells.
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f4-bt-22-477: Detection of oxidized DNA bases, namely (A) 8-hydroxy-2′-deoxyguanosine (8-OHdG) and (B) N6-etheno-2-deoxyadenosine after treatment of HaCaT keratinocytes with the kINPen 09 or hydrogen peroxide (100 μM). 24 h after exposure of cells to plasma they were fixed and stained with the antibodies 2E2 for 8-OHdG for and EMA-1 for N6-etheno-2′-desoxyadenosine. Binding of antibodies was detected by using flow cytometry. Mean fluorescence intensities (MFI) are expressed as percentage of that of untreated control cells. Mean ± SEM, *p<0.05 vs. untreated control cells.

Mentions: DNA base changes were observed after exposing HaCaT cells to the plasma jet kINPen 09 (Fig. 4). Flow cytometry was used to detect binding of corresponding antibodies, EMA-1 for N6-etheno-2′-desoxyadenosine and 2E2 for 8-hydroxy-2′-deoxyguanosine. While N6-etheno-2′-desoxyadenosine was found to be significantly increased by hydrogen peroxide and kINPen 09 treatment for at least 120 s (Fig. 4A), 8-hydroxy-2′-deoxyguanosine was only slightly enhanced after hydrogen peroxide and 180 s kINPen 09 exposure (Fig. 4B). These different results might be due to the fact that 8-hydroxy-2′-deoxyguanosine is the result from oxidation, while the DNA adduct N6-etheno-2′-deoxyadenosine arises from reaction of DNA with lipid peroxidation products (Taghizadeh et al., 2008). Lipid peroxidation can be the result of plasma treatment due to ROS generation. Transient increased expression of 8-hydroxy-2′-deoxyguanosine was also seen by Brun et al. (2012) in ocular keratocytes after exposing them to plasma.


Non-thermal atmospheric-pressure plasma possible application in wound healing.

Haertel B, von Woedtke T, Weltmann KD, Lindequist U - Biomol Ther (Seoul) (2014)

Detection of oxidized DNA bases, namely (A) 8-hydroxy-2′-deoxyguanosine (8-OHdG) and (B) N6-etheno-2-deoxyadenosine after treatment of HaCaT keratinocytes with the kINPen 09 or hydrogen peroxide (100 μM). 24 h after exposure of cells to plasma they were fixed and stained with the antibodies 2E2 for 8-OHdG for and EMA-1 for N6-etheno-2′-desoxyadenosine. Binding of antibodies was detected by using flow cytometry. Mean fluorescence intensities (MFI) are expressed as percentage of that of untreated control cells. Mean ± SEM, *p<0.05 vs. untreated control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256026&req=5

f4-bt-22-477: Detection of oxidized DNA bases, namely (A) 8-hydroxy-2′-deoxyguanosine (8-OHdG) and (B) N6-etheno-2-deoxyadenosine after treatment of HaCaT keratinocytes with the kINPen 09 or hydrogen peroxide (100 μM). 24 h after exposure of cells to plasma they were fixed and stained with the antibodies 2E2 for 8-OHdG for and EMA-1 for N6-etheno-2′-desoxyadenosine. Binding of antibodies was detected by using flow cytometry. Mean fluorescence intensities (MFI) are expressed as percentage of that of untreated control cells. Mean ± SEM, *p<0.05 vs. untreated control cells.
Mentions: DNA base changes were observed after exposing HaCaT cells to the plasma jet kINPen 09 (Fig. 4). Flow cytometry was used to detect binding of corresponding antibodies, EMA-1 for N6-etheno-2′-desoxyadenosine and 2E2 for 8-hydroxy-2′-deoxyguanosine. While N6-etheno-2′-desoxyadenosine was found to be significantly increased by hydrogen peroxide and kINPen 09 treatment for at least 120 s (Fig. 4A), 8-hydroxy-2′-deoxyguanosine was only slightly enhanced after hydrogen peroxide and 180 s kINPen 09 exposure (Fig. 4B). These different results might be due to the fact that 8-hydroxy-2′-deoxyguanosine is the result from oxidation, while the DNA adduct N6-etheno-2′-deoxyadenosine arises from reaction of DNA with lipid peroxidation products (Taghizadeh et al., 2008). Lipid peroxidation can be the result of plasma treatment due to ROS generation. Transient increased expression of 8-hydroxy-2′-deoxyguanosine was also seen by Brun et al. (2012) in ocular keratocytes after exposing them to plasma.

Bottom Line: Therefore, it cannot be equated with plasma from blood; it is not biological in nature.This review emphasizes plasma effects on wound healing.We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy, Ernst-Moritz-Arndt University of Greifswald, D17489 Greifswald, Germany.

ABSTRACT
Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

No MeSH data available.


Related in: MedlinePlus