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Non-thermal atmospheric-pressure plasma possible application in wound healing.

Haertel B, von Woedtke T, Weltmann KD, Lindequist U - Biomol Ther (Seoul) (2014)

Bottom Line: Therefore, it cannot be equated with plasma from blood; it is not biological in nature.This review emphasizes plasma effects on wound healing.We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy, Ernst-Moritz-Arndt University of Greifswald, D17489 Greifswald, Germany.

ABSTRACT
Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

No MeSH data available.


Related in: MedlinePlus

Principle way of reactive oxygen and nitrogen species from an argon plasma jet over the gas phase and liquid into the treated cells. Since plasma jets are open systems the effluents are surrounded by ambient air with its gases N2, O2 and CO2. Some species are exemplified shown. Species can enter the cell possibly by diffusion or can induce new species within the cells.
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f3-bt-22-477: Principle way of reactive oxygen and nitrogen species from an argon plasma jet over the gas phase and liquid into the treated cells. Since plasma jets are open systems the effluents are surrounded by ambient air with its gases N2, O2 and CO2. Some species are exemplified shown. Species can enter the cell possibly by diffusion or can induce new species within the cells.

Mentions: As already mentioned plasma emits several kinds of radiation and is further characterized by reactive oxygen and nitrogen species (ROS and RNS, Schaper et al., 2009; Schmidt-Bleker et al., 2014; Oehmigen, 2014). Among them, e.g. ozone/O3 (Reuter et al., 2012b), nitric oxide/NO (Pipa et al., 2012), atomic oxygen/O (Reuter et al., 2012a), and hydroxyl radical /·HO (Winter et al., 2014) were detected. These reactive species exert lots of effects on cells which can be positive or even negative (Table 3). After treatment of cells in culture medium reactive species are detectable in the gas phase over the cells as well as in the culture medium (Fig. 3). By using an argon plasma jet the effluent is surrounded by ambient air, thereby in the gas phase are not only argon atoms but also species built from ambient air. Furthermore, all species can also enter the cells possibly by diffusion or can induce new species within the cells (Fig. 3). These can be detected as intracellular ROS (iROS) by different fluorescent dyes (DAF-2: Arjunan et al., 2011a; H2DCFDA: Brun et al., 2012; Haertel et al., 2012a; CM-H2DCFDA: Haertel et al., 2013b; carboxy-H2DCFDA: Leduc et al., 2010; Ma et al., 2014).


Non-thermal atmospheric-pressure plasma possible application in wound healing.

Haertel B, von Woedtke T, Weltmann KD, Lindequist U - Biomol Ther (Seoul) (2014)

Principle way of reactive oxygen and nitrogen species from an argon plasma jet over the gas phase and liquid into the treated cells. Since plasma jets are open systems the effluents are surrounded by ambient air with its gases N2, O2 and CO2. Some species are exemplified shown. Species can enter the cell possibly by diffusion or can induce new species within the cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256026&req=5

f3-bt-22-477: Principle way of reactive oxygen and nitrogen species from an argon plasma jet over the gas phase and liquid into the treated cells. Since plasma jets are open systems the effluents are surrounded by ambient air with its gases N2, O2 and CO2. Some species are exemplified shown. Species can enter the cell possibly by diffusion or can induce new species within the cells.
Mentions: As already mentioned plasma emits several kinds of radiation and is further characterized by reactive oxygen and nitrogen species (ROS and RNS, Schaper et al., 2009; Schmidt-Bleker et al., 2014; Oehmigen, 2014). Among them, e.g. ozone/O3 (Reuter et al., 2012b), nitric oxide/NO (Pipa et al., 2012), atomic oxygen/O (Reuter et al., 2012a), and hydroxyl radical /·HO (Winter et al., 2014) were detected. These reactive species exert lots of effects on cells which can be positive or even negative (Table 3). After treatment of cells in culture medium reactive species are detectable in the gas phase over the cells as well as in the culture medium (Fig. 3). By using an argon plasma jet the effluent is surrounded by ambient air, thereby in the gas phase are not only argon atoms but also species built from ambient air. Furthermore, all species can also enter the cells possibly by diffusion or can induce new species within the cells (Fig. 3). These can be detected as intracellular ROS (iROS) by different fluorescent dyes (DAF-2: Arjunan et al., 2011a; H2DCFDA: Brun et al., 2012; Haertel et al., 2012a; CM-H2DCFDA: Haertel et al., 2013b; carboxy-H2DCFDA: Leduc et al., 2010; Ma et al., 2014).

Bottom Line: Therefore, it cannot be equated with plasma from blood; it is not biological in nature.This review emphasizes plasma effects on wound healing.We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy, Ernst-Moritz-Arndt University of Greifswald, D17489 Greifswald, Germany.

ABSTRACT
Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

No MeSH data available.


Related in: MedlinePlus