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Comprehensive transcriptome analysis of early male and female Bactrocera jarvisi embryos.

Morrow JL, Riegler M, Gilchrist AS, Shearman DC, Frommer M - BMC Genet. (2014)

Bottom Line: No strong candidates for transcripts derived solely from the Y chromosome were recovered from the poly(A+) fraction.Bactrocera jarvisi provides an excellent model for embryonic studies due to available Y-chromosome markers and the compact time frame for zygotic transcription and the sex-determined state.Our data contribute fundamental information to sex-determination research, and provide candidates for the sourcing of gene promoters for transgenic pest-management strategies of tephritid fruit flies.

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ABSTRACT

Background: Developing embryos are provided with maternal RNA transcripts and proteins, but transcription from the zygotic nuclei must be activated to control continuing embryonic development. Transcripts are generated at different stages of early development, and those involved in sex determination and cellularisation are some of the earliest to be activated. The male sex in tephritid fruit flies is determined by the presence of a Y chromosome, and it is believed that a transcript from the Y-chromosome sets in motion a cascade that determines male development, as part of the greater maternal to zygotic transition (MTZ). Here we investigate the poly(A+) transcriptome in early male and female embryos of the horticultural pest Bactrocera jarvisi (Diptera: Tephritidae).

Results: Bactrocera jarvisi embryos were collected over two pre-blastoderm time periods, 2-3h and 3-5h after egg laying. Embryos were individually sexed using a Y-chromosome marker, allowing the sex-specific poly(A+) transcriptome of single-sex embryo pools to be deep-sequenced and assembled de novo. Transcripts for sixteen sex-determination and two cellularisation gene homologues of Drosophila melanogaster (Diptera: Drosophilidae) were identified in early embryos of B. jarvisi, including transcripts highly upregulated prior to cellularisation. No strong candidates for transcripts derived solely from the Y chromosome were recovered from the poly(A+) fraction.

Conclusions: Bactrocera jarvisi provides an excellent model for embryonic studies due to available Y-chromosome markers and the compact time frame for zygotic transcription and the sex-determined state. Our data contribute fundamental information to sex-determination research, and provide candidates for the sourcing of gene promoters for transgenic pest-management strategies of tephritid fruit flies.

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Classification of the gene ontology (GO) terms for the B. jarvisi transcriptome. GO was implemented in BLAST2GO on a subset of the CLC assembly comprising 23,518 contigs. Of these, 8,864 transcripts (45.6%) were classified into 37 functional groups.
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Figure 1: Classification of the gene ontology (GO) terms for the B. jarvisi transcriptome. GO was implemented in BLAST2GO on a subset of the CLC assembly comprising 23,518 contigs. Of these, 8,864 transcripts (45.6%) were classified into 37 functional groups.

Mentions: Gene ontology (GO) analysis annotated 8,864 transcripts (45.6%), categorised into 37 functional groups within the classes molecular function, cellular component and biological process (Figure 1). The largest representations were in "binding" and "catalytic activity" (molecular function), "cell" (cellular component) and "cellular process", "metabolic process" and "single-organism process" (biological process). Within biological processes, 1,108 sequences mapped to developmental processes (GO:0032502); 274 sequences were developmental processes involved in reproduction (GO:0003006) and 13 sequences mapped to sex determination (GO:0007530).


Comprehensive transcriptome analysis of early male and female Bactrocera jarvisi embryos.

Morrow JL, Riegler M, Gilchrist AS, Shearman DC, Frommer M - BMC Genet. (2014)

Classification of the gene ontology (GO) terms for the B. jarvisi transcriptome. GO was implemented in BLAST2GO on a subset of the CLC assembly comprising 23,518 contigs. Of these, 8,864 transcripts (45.6%) were classified into 37 functional groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255828&req=5

Figure 1: Classification of the gene ontology (GO) terms for the B. jarvisi transcriptome. GO was implemented in BLAST2GO on a subset of the CLC assembly comprising 23,518 contigs. Of these, 8,864 transcripts (45.6%) were classified into 37 functional groups.
Mentions: Gene ontology (GO) analysis annotated 8,864 transcripts (45.6%), categorised into 37 functional groups within the classes molecular function, cellular component and biological process (Figure 1). The largest representations were in "binding" and "catalytic activity" (molecular function), "cell" (cellular component) and "cellular process", "metabolic process" and "single-organism process" (biological process). Within biological processes, 1,108 sequences mapped to developmental processes (GO:0032502); 274 sequences were developmental processes involved in reproduction (GO:0003006) and 13 sequences mapped to sex determination (GO:0007530).

Bottom Line: No strong candidates for transcripts derived solely from the Y chromosome were recovered from the poly(A+) fraction.Bactrocera jarvisi provides an excellent model for embryonic studies due to available Y-chromosome markers and the compact time frame for zygotic transcription and the sex-determined state.Our data contribute fundamental information to sex-determination research, and provide candidates for the sourcing of gene promoters for transgenic pest-management strategies of tephritid fruit flies.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Developing embryos are provided with maternal RNA transcripts and proteins, but transcription from the zygotic nuclei must be activated to control continuing embryonic development. Transcripts are generated at different stages of early development, and those involved in sex determination and cellularisation are some of the earliest to be activated. The male sex in tephritid fruit flies is determined by the presence of a Y chromosome, and it is believed that a transcript from the Y-chromosome sets in motion a cascade that determines male development, as part of the greater maternal to zygotic transition (MTZ). Here we investigate the poly(A+) transcriptome in early male and female embryos of the horticultural pest Bactrocera jarvisi (Diptera: Tephritidae).

Results: Bactrocera jarvisi embryos were collected over two pre-blastoderm time periods, 2-3h and 3-5h after egg laying. Embryos were individually sexed using a Y-chromosome marker, allowing the sex-specific poly(A+) transcriptome of single-sex embryo pools to be deep-sequenced and assembled de novo. Transcripts for sixteen sex-determination and two cellularisation gene homologues of Drosophila melanogaster (Diptera: Drosophilidae) were identified in early embryos of B. jarvisi, including transcripts highly upregulated prior to cellularisation. No strong candidates for transcripts derived solely from the Y chromosome were recovered from the poly(A+) fraction.

Conclusions: Bactrocera jarvisi provides an excellent model for embryonic studies due to available Y-chromosome markers and the compact time frame for zygotic transcription and the sex-determined state. Our data contribute fundamental information to sex-determination research, and provide candidates for the sourcing of gene promoters for transgenic pest-management strategies of tephritid fruit flies.

Show MeSH
Related in: MedlinePlus