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Increased microvascular vasodilation and cardiovascular risk following a pre-eclamptic pregnancy.

Murphy MS, Vignarajah M, Smith GN - Physiol Rep (2014)

Bottom Line: Acetylcholine-mediated vasodilation was enhanced by normotensive pregnancy, and declined to nonpregnant levels by 6 months postpartum.Acetylcholine-mediated vasodilation remained high in pre-eclamptic subjects from 2 to 6 months postpartum compared to normotensive and never-pregnant controls.Pre-eclamptic subjects exhibited elevated 30-year and lifetime risk at 6 months postpartum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical and Molecular Science, Queen's University, Kingston, Ontario, Canada.

No MeSH data available.


Related in: MedlinePlus

A representative depiction of dose‐response recordings generated from the application of laser Doppler Flowmetry and iontophoresis. Linear graphs correspond to changes in blood flow in response to (A) 1% SNP and (B) 1% ACh diluted in deionized water.
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fig01: A representative depiction of dose‐response recordings generated from the application of laser Doppler Flowmetry and iontophoresis. Linear graphs correspond to changes in blood flow in response to (A) 1% SNP and (B) 1% ACh diluted in deionized water.

Mentions: Iontophoresis is a technique used for the noninvasive delivery of drug solutions across the skin. The principle is based on the movement of charged ions across the skin in the presence of an applied electrical field. The magnitude of charge (Q) is dependent on size of the current (I), and corresponds to the amount of drug delivered. 1% solutions of acetylcholine (ACh; Miochol®‐E, Bausch&Lomb Inc., Vaughan, ON, Canada) and sodium nitroprusside (SNP; Nipride, Hospira Inc., Saint‐Laurent, QC, Canada) were introduced into the anodal and cathodal chambers for assessment of endothelial‐dependent and ‐independent function respectively. The vehicle for drug delivery was deionized sterile water. Following a 10‐min period of stable baseline perfusion recordings, dose‐response curves to ACh and SNP were obtained by the step‐wise application of currents (Davis et al. 2001) (5 μA, 10 μA, 15 μA, 20 μA, 50 μA, and three applications of 100 μA) by an iontophoresis controller (MIC2, Moor Instruments Ltd.). Currents were applied for 10 sec followed by 2 min recording periods for a total charge delivery of 4 mC. Corresponding changes in cutaneous blood flow was assessed using the laser Doppler flow monitor (moorVMS‐LDF, Moor Instruments Ltd.) (Fig. 1). Vasodilation was taken as the ratio of peak flux to an average of the total 10 min baseline perfusion for each drug administered per iontophoretic dose.


Increased microvascular vasodilation and cardiovascular risk following a pre-eclamptic pregnancy.

Murphy MS, Vignarajah M, Smith GN - Physiol Rep (2014)

A representative depiction of dose‐response recordings generated from the application of laser Doppler Flowmetry and iontophoresis. Linear graphs correspond to changes in blood flow in response to (A) 1% SNP and (B) 1% ACh diluted in deionized water.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255821&req=5

fig01: A representative depiction of dose‐response recordings generated from the application of laser Doppler Flowmetry and iontophoresis. Linear graphs correspond to changes in blood flow in response to (A) 1% SNP and (B) 1% ACh diluted in deionized water.
Mentions: Iontophoresis is a technique used for the noninvasive delivery of drug solutions across the skin. The principle is based on the movement of charged ions across the skin in the presence of an applied electrical field. The magnitude of charge (Q) is dependent on size of the current (I), and corresponds to the amount of drug delivered. 1% solutions of acetylcholine (ACh; Miochol®‐E, Bausch&Lomb Inc., Vaughan, ON, Canada) and sodium nitroprusside (SNP; Nipride, Hospira Inc., Saint‐Laurent, QC, Canada) were introduced into the anodal and cathodal chambers for assessment of endothelial‐dependent and ‐independent function respectively. The vehicle for drug delivery was deionized sterile water. Following a 10‐min period of stable baseline perfusion recordings, dose‐response curves to ACh and SNP were obtained by the step‐wise application of currents (Davis et al. 2001) (5 μA, 10 μA, 15 μA, 20 μA, 50 μA, and three applications of 100 μA) by an iontophoresis controller (MIC2, Moor Instruments Ltd.). Currents were applied for 10 sec followed by 2 min recording periods for a total charge delivery of 4 mC. Corresponding changes in cutaneous blood flow was assessed using the laser Doppler flow monitor (moorVMS‐LDF, Moor Instruments Ltd.) (Fig. 1). Vasodilation was taken as the ratio of peak flux to an average of the total 10 min baseline perfusion for each drug administered per iontophoretic dose.

Bottom Line: Acetylcholine-mediated vasodilation was enhanced by normotensive pregnancy, and declined to nonpregnant levels by 6 months postpartum.Acetylcholine-mediated vasodilation remained high in pre-eclamptic subjects from 2 to 6 months postpartum compared to normotensive and never-pregnant controls.Pre-eclamptic subjects exhibited elevated 30-year and lifetime risk at 6 months postpartum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical and Molecular Science, Queen's University, Kingston, Ontario, Canada.

No MeSH data available.


Related in: MedlinePlus