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Preserved functional autonomic phenotype in adult mice overexpressing moderate levels of human alpha-synuclein in oligodendrocytes.

Tank J, da Costa-Goncalves AC, Kamer I, Qadri F, Ubhi K, Rockenstein E, Diedrich A, Masliah E, Gross V, Jordan J - Physiol Rep (2014)

Bottom Line: HR responses to atropine (+159 ± 24 vs. +146 ± 22 beats/min), and to clonidine (-188 ± 21 vs. -163 ± 33 beats/min) did not differ between strains.Baroreflex sensitivity (4 ± 1 vs. 4 ± 1 msec/mmHg) and HR variability (total power, 84 ± 17 vs. 65 ± 21 msec²) were similar under resting conditions and during pharmacological testing.Repeated measurements at 12 months of age provided similar results.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany.

No MeSH data available.


Related in: MedlinePlus

ChAT immunoreactivity in the nucleus ambiguus of nontransgenic and MBP1‐α‐syn tg mice (A). Serial vibratome sections from wild‐type (n = 6) and MBP1‐α‐syn tg mice (n = 6) were immunolabeled with the rabbit polyclonal antibody against ChAT and analyzed by the dissector method with the MBL serology system. Discrete groups of ChAT‐positive cells were identified, in both groups, compared to wild type the MBP1‐α‐syn tg mice showed a nonsignificant trend (B). Bar = 25 µm.
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fig02: ChAT immunoreactivity in the nucleus ambiguus of nontransgenic and MBP1‐α‐syn tg mice (A). Serial vibratome sections from wild‐type (n = 6) and MBP1‐α‐syn tg mice (n = 6) were immunolabeled with the rabbit polyclonal antibody against ChAT and analyzed by the dissector method with the MBL serology system. Discrete groups of ChAT‐positive cells were identified, in both groups, compared to wild type the MBP1‐α‐syn tg mice showed a nonsignificant trend (B). Bar = 25 µm.

Mentions: Transgenic MBP1‐α‐syn showed a trend of reduced ChAT‐positive cells in the nucleus ambiguous compared to wild‐type mice (Fig. 2, P = 0.1028). Mean arterial blood pressure responses to trimethaphan (tg: −21 ± 8 vs. wt: −10 ± 5 mmHg, P = 0.240) and to clonidine (tg: −8 ± 3 vs. wt: −5 ± 2 mmHg, P = 0.314) were similar in both strains. HR responses to trimethaphan (tg: −90 ± 25 vs. wt: −98 ± 22 beats/min), and to clonidine (tg: −188 ± 21 vs. wt: −163 ± 33 beats/min) did not differ between strains. Figure 3 shows the BP and HR values measured for 1 h immediately after the drug injection during pharmacological testing at 9 months of age. We obtained similar results during pharmacological testing at 9 and 12 months of age. The time courses of BP and HR responses were almost identical in both strains.


Preserved functional autonomic phenotype in adult mice overexpressing moderate levels of human alpha-synuclein in oligodendrocytes.

Tank J, da Costa-Goncalves AC, Kamer I, Qadri F, Ubhi K, Rockenstein E, Diedrich A, Masliah E, Gross V, Jordan J - Physiol Rep (2014)

ChAT immunoreactivity in the nucleus ambiguus of nontransgenic and MBP1‐α‐syn tg mice (A). Serial vibratome sections from wild‐type (n = 6) and MBP1‐α‐syn tg mice (n = 6) were immunolabeled with the rabbit polyclonal antibody against ChAT and analyzed by the dissector method with the MBL serology system. Discrete groups of ChAT‐positive cells were identified, in both groups, compared to wild type the MBP1‐α‐syn tg mice showed a nonsignificant trend (B). Bar = 25 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255815&req=5

fig02: ChAT immunoreactivity in the nucleus ambiguus of nontransgenic and MBP1‐α‐syn tg mice (A). Serial vibratome sections from wild‐type (n = 6) and MBP1‐α‐syn tg mice (n = 6) were immunolabeled with the rabbit polyclonal antibody against ChAT and analyzed by the dissector method with the MBL serology system. Discrete groups of ChAT‐positive cells were identified, in both groups, compared to wild type the MBP1‐α‐syn tg mice showed a nonsignificant trend (B). Bar = 25 µm.
Mentions: Transgenic MBP1‐α‐syn showed a trend of reduced ChAT‐positive cells in the nucleus ambiguous compared to wild‐type mice (Fig. 2, P = 0.1028). Mean arterial blood pressure responses to trimethaphan (tg: −21 ± 8 vs. wt: −10 ± 5 mmHg, P = 0.240) and to clonidine (tg: −8 ± 3 vs. wt: −5 ± 2 mmHg, P = 0.314) were similar in both strains. HR responses to trimethaphan (tg: −90 ± 25 vs. wt: −98 ± 22 beats/min), and to clonidine (tg: −188 ± 21 vs. wt: −163 ± 33 beats/min) did not differ between strains. Figure 3 shows the BP and HR values measured for 1 h immediately after the drug injection during pharmacological testing at 9 months of age. We obtained similar results during pharmacological testing at 9 and 12 months of age. The time courses of BP and HR responses were almost identical in both strains.

Bottom Line: HR responses to atropine (+159 ± 24 vs. +146 ± 22 beats/min), and to clonidine (-188 ± 21 vs. -163 ± 33 beats/min) did not differ between strains.Baroreflex sensitivity (4 ± 1 vs. 4 ± 1 msec/mmHg) and HR variability (total power, 84 ± 17 vs. 65 ± 21 msec²) were similar under resting conditions and during pharmacological testing.Repeated measurements at 12 months of age provided similar results.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany.

No MeSH data available.


Related in: MedlinePlus