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DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus

Confirmation of differentially modified transcripts form microarray analysis. BDNF signalling‐related genes and most up‐ or down‐regulated by WR or RWR were selected for validation via quantitative real‐time PCR. Each column indicates WR (diagonal stripes columns) or RWR (black columns), respectively. For each gene, the expression change is represented as the fold change in WR or RWR relative to Sedentary.
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fig06: Confirmation of differentially modified transcripts form microarray analysis. BDNF signalling‐related genes and most up‐ or down‐regulated by WR or RWR were selected for validation via quantitative real‐time PCR. Each column indicates WR (diagonal stripes columns) or RWR (black columns), respectively. For each gene, the expression change is represented as the fold change in WR or RWR relative to Sedentary.

Mentions: To validate gene changes in DNA microarray data and test the robustness of these responses, quantitative RT‐PCR was used to assess expression of select transcripts. We selected 11 genes with annotated functions that were highly up‐regulated or down‐regulated at the sedentary x RWR condition. As a result of their potential relevance to cognitive functions, transcripts involved in BDNF signaling were also analyzed. The mRNA expression profiles revealed that the DNA microarray data could be validated using appropriate primer design (Fig. 6).


DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

Confirmation of differentially modified transcripts form microarray analysis. BDNF signalling‐related genes and most up‐ or down‐regulated by WR or RWR were selected for validation via quantitative real‐time PCR. Each column indicates WR (diagonal stripes columns) or RWR (black columns), respectively. For each gene, the expression change is represented as the fold change in WR or RWR relative to Sedentary.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255813&req=5

fig06: Confirmation of differentially modified transcripts form microarray analysis. BDNF signalling‐related genes and most up‐ or down‐regulated by WR or RWR were selected for validation via quantitative real‐time PCR. Each column indicates WR (diagonal stripes columns) or RWR (black columns), respectively. For each gene, the expression change is represented as the fold change in WR or RWR relative to Sedentary.
Mentions: To validate gene changes in DNA microarray data and test the robustness of these responses, quantitative RT‐PCR was used to assess expression of select transcripts. We selected 11 genes with annotated functions that were highly up‐regulated or down‐regulated at the sedentary x RWR condition. As a result of their potential relevance to cognitive functions, transcripts involved in BDNF signaling were also analyzed. The mRNA expression profiles revealed that the DNA microarray data could be validated using appropriate primer design (Fig. 6).

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus