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DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus

The two top gene networks sensitive to free wheel running. Ingenuity Pathway Analysis software was used to identify the most significantly modified gene networks from Sed × WR. (A) Network 1 is involved in Amino Acid Metabolism, Endocrine System Development and Function, Molecular Transport. (B) Network 2 is involved in Protein Synthesis, Gene Expression, Cellular Movement. Transcripts are color‐coded, according to expression changes (red, up‐regulation; green, down‐regulation). White indicates predicted molecules computational incorporated into networks based on evidence within the IPA knowledge base. Lines between molecules indicate a direct molecular connection.
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fig04: The two top gene networks sensitive to free wheel running. Ingenuity Pathway Analysis software was used to identify the most significantly modified gene networks from Sed × WR. (A) Network 1 is involved in Amino Acid Metabolism, Endocrine System Development and Function, Molecular Transport. (B) Network 2 is involved in Protein Synthesis, Gene Expression, Cellular Movement. Transcripts are color‐coded, according to expression changes (red, up‐regulation; green, down‐regulation). White indicates predicted molecules computational incorporated into networks based on evidence within the IPA knowledge base. Lines between molecules indicate a direct molecular connection.

Mentions: Functional annotation of differentially expressed genes was performed using the IPA platform. The majority of these changes were unique to exercise mode (free wheel running or resistance wheel running), reflecting 4 weeks of the experiment. Table 3 lists the 10 most induced and repressed transcripts of the RWR group. In addition to this pathway, we analyzed the molecular connection between each hippocampal gene based on previous literature to gain further insight and to characterize the molecular behavior regulating hippocampal neuroplasticity at each intensity (Table 4). Networks were generated on the basis of known functions and interconnectivity of affected genes. The top five gene networks were constructed on the basis of the known functions and interconnectivity of altered genes (Table 5). WR‐regulated genes were involved in amino acid metabolism, endocrine system and molecular transport; however, RWR were involved in inflammatory/immune response, protein synthesis, and cellular movement. This was reflected in the top two modified molecular networks (Figs. 4, 5).


DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

The two top gene networks sensitive to free wheel running. Ingenuity Pathway Analysis software was used to identify the most significantly modified gene networks from Sed × WR. (A) Network 1 is involved in Amino Acid Metabolism, Endocrine System Development and Function, Molecular Transport. (B) Network 2 is involved in Protein Synthesis, Gene Expression, Cellular Movement. Transcripts are color‐coded, according to expression changes (red, up‐regulation; green, down‐regulation). White indicates predicted molecules computational incorporated into networks based on evidence within the IPA knowledge base. Lines between molecules indicate a direct molecular connection.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255813&req=5

fig04: The two top gene networks sensitive to free wheel running. Ingenuity Pathway Analysis software was used to identify the most significantly modified gene networks from Sed × WR. (A) Network 1 is involved in Amino Acid Metabolism, Endocrine System Development and Function, Molecular Transport. (B) Network 2 is involved in Protein Synthesis, Gene Expression, Cellular Movement. Transcripts are color‐coded, according to expression changes (red, up‐regulation; green, down‐regulation). White indicates predicted molecules computational incorporated into networks based on evidence within the IPA knowledge base. Lines between molecules indicate a direct molecular connection.
Mentions: Functional annotation of differentially expressed genes was performed using the IPA platform. The majority of these changes were unique to exercise mode (free wheel running or resistance wheel running), reflecting 4 weeks of the experiment. Table 3 lists the 10 most induced and repressed transcripts of the RWR group. In addition to this pathway, we analyzed the molecular connection between each hippocampal gene based on previous literature to gain further insight and to characterize the molecular behavior regulating hippocampal neuroplasticity at each intensity (Table 4). Networks were generated on the basis of known functions and interconnectivity of affected genes. The top five gene networks were constructed on the basis of the known functions and interconnectivity of altered genes (Table 5). WR‐regulated genes were involved in amino acid metabolism, endocrine system and molecular transport; however, RWR were involved in inflammatory/immune response, protein synthesis, and cellular movement. This was reflected in the top two modified molecular networks (Figs. 4, 5).

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus