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DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus

Biological pathway and gene classification. The up‐ and down‐regulated genes at Sed × WR and Sed × RWR were classified based on the available categories of more than 100 biological pathways and gene classification in the SABiosciences PCR array list. The numbers in the y‐axis represent number of genes in each category, which are indicated on the x‐axis.
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fig03: Biological pathway and gene classification. The up‐ and down‐regulated genes at Sed × WR and Sed × RWR were classified based on the available categories of more than 100 biological pathways and gene classification in the SABiosciences PCR array list. The numbers in the y‐axis represent number of genes in each category, which are indicated on the x‐axis.

Mentions: The pathway‐focused gene classifications available on the QIAGEN website (SABiosciences; www.sabiosciences.com) were utilized to reveal the trend of predominant pathways affected in the hippocampus (Fig. 3). The up‐ and down‐regulated genes at sedentary × WR, and sedentary × RWR were classified based on the available categories of more than 100 biological pathways at hippocampus after 4 weeks of running. This categorization revealed that the trends of gene quantity (numbers) and quality (function) in the respective pathways varied between the two running modes.


DNA microarray-based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity.

Lee MC, Rakwal R, Shibato J, Inoue K, Chang H, Soya H - Physiol Rep (2014)

Biological pathway and gene classification. The up‐ and down‐regulated genes at Sed × WR and Sed × RWR were classified based on the available categories of more than 100 biological pathways and gene classification in the SABiosciences PCR array list. The numbers in the y‐axis represent number of genes in each category, which are indicated on the x‐axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255813&req=5

fig03: Biological pathway and gene classification. The up‐ and down‐regulated genes at Sed × WR and Sed × RWR were classified based on the available categories of more than 100 biological pathways and gene classification in the SABiosciences PCR array list. The numbers in the y‐axis represent number of genes in each category, which are indicated on the x‐axis.
Mentions: The pathway‐focused gene classifications available on the QIAGEN website (SABiosciences; www.sabiosciences.com) were utilized to reveal the trend of predominant pathways affected in the hippocampus (Fig. 3). The up‐ and down‐regulated genes at sedentary × WR, and sedentary × RWR were classified based on the available categories of more than 100 biological pathways at hippocampus after 4 weeks of running. This categorization revealed that the trends of gene quantity (numbers) and quality (function) in the respective pathways varied between the two running modes.

Bottom Line: RWR rats showed a significant decrease in average running distances although average work levels increased immensely, by about 11-fold compared to WR, resulting in muscular adaptation for the fast-twitch plantaris muscle.Interestingly, RWR down-regulated multiple inflammatory cytokines (IL1B, IL2RA, and TNF) and chemokines (CXCL1, CXCL10, CCL2, and CCR4) with the SYCP3, PRL genes, which are potentially involved in regulating hippocampal neuroplastic changes.These results provide understanding of the voluntary-RWR-related hippocampal transcriptome, which will open a window to the underlying mechanisms of the positive effects of exercise, with therapeutic value for enhancing hippocampal functions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, TsukubaIbaraki, Japan International Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.

No MeSH data available.


Related in: MedlinePlus