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Enhanced production of IGF-I in the lungs of fibroproliferative ARDS patients.

Andonegui G, Krein PM, Mowat C, Brisebois R, Doig C, Green FH, Léger C, Winston BW - Physiol Rep (2014)

Bottom Line: Our data show that IGF-I is significantly increased in the ELF in FP-ARDS patients.A significant correlation between IGF-I and PCP-III in the ELF of FP-ARDS patients is found.Our data suggest that IGF-I found in the lungs of FP-ARDS patients results from both increased lung permeability and local production of IGF-I.

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care Medicine, Faculty of Medicine, University of Calgary Health Research Innovation Center, Calgary, Alberta, Canada Immunology Research Group, Faculty of Medicine, University of Calgary Health Research Innovation Center, Calgary, Alberta, Canada.

No MeSH data available.


Related in: MedlinePlus

PCP‐III Protein in ELF. PCP‐III protein levels measured in BALF by RIA. ELF concentration corrected for dilution by the urea method as described in Materials and Methods. (A) PCP‐III protein levels are increased in the ELF of FP‐ARDS individuals (n = 14) versus control individuals (n = 11), *P < 0.01, 95% CI and versus early ALI/ARDS individuals (n = 9), #P < 0.05, 95% CI. (B) A significant positive relationship exists between IGF‐I and PCP‐III levels in the ELF (P < 0.01, 95% CI) of FP‐ARDS. IGF‐I and PCP‐III levels were analyzed by Spearman Rank Correlation analysis as described in Materials and Methods.
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fig02: PCP‐III Protein in ELF. PCP‐III protein levels measured in BALF by RIA. ELF concentration corrected for dilution by the urea method as described in Materials and Methods. (A) PCP‐III protein levels are increased in the ELF of FP‐ARDS individuals (n = 14) versus control individuals (n = 11), *P < 0.01, 95% CI and versus early ALI/ARDS individuals (n = 9), #P < 0.05, 95% CI. (B) A significant positive relationship exists between IGF‐I and PCP‐III levels in the ELF (P < 0.01, 95% CI) of FP‐ARDS. IGF‐I and PCP‐III levels were analyzed by Spearman Rank Correlation analysis as described in Materials and Methods.

Mentions: IGF‐I has been shown to induce collagen production in fibroblasts in vitro (Goldstein et al. 1989; Olbruck et al. 1998), suggesting one of the consequences of enhanced IGF‐I protein in the lungs of FP‐ARDS patients may be increased collagen deposition in this organ. Therefore, we measured PCP‐III, a byproduct of collagen III processing as a surrogate for collagen deposition in the lungs. Figure 2A shows increased PCP‐III levels in the ELF from FP‐ARDS patients compared to both early ALI/ARDS and controls. Importantly, the protein levels of IGF‐I and PCP‐III in the ELF of FP‐ARDS are significantly correlated versus control individuals (P < 0.01) (Fig. 2B). These results further support the hypothesis that IGF‐I may induce or contribute to collagen production and deposition in the lungs of FP‐ARDS patients.


Enhanced production of IGF-I in the lungs of fibroproliferative ARDS patients.

Andonegui G, Krein PM, Mowat C, Brisebois R, Doig C, Green FH, Léger C, Winston BW - Physiol Rep (2014)

PCP‐III Protein in ELF. PCP‐III protein levels measured in BALF by RIA. ELF concentration corrected for dilution by the urea method as described in Materials and Methods. (A) PCP‐III protein levels are increased in the ELF of FP‐ARDS individuals (n = 14) versus control individuals (n = 11), *P < 0.01, 95% CI and versus early ALI/ARDS individuals (n = 9), #P < 0.05, 95% CI. (B) A significant positive relationship exists between IGF‐I and PCP‐III levels in the ELF (P < 0.01, 95% CI) of FP‐ARDS. IGF‐I and PCP‐III levels were analyzed by Spearman Rank Correlation analysis as described in Materials and Methods.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255805&req=5

fig02: PCP‐III Protein in ELF. PCP‐III protein levels measured in BALF by RIA. ELF concentration corrected for dilution by the urea method as described in Materials and Methods. (A) PCP‐III protein levels are increased in the ELF of FP‐ARDS individuals (n = 14) versus control individuals (n = 11), *P < 0.01, 95% CI and versus early ALI/ARDS individuals (n = 9), #P < 0.05, 95% CI. (B) A significant positive relationship exists between IGF‐I and PCP‐III levels in the ELF (P < 0.01, 95% CI) of FP‐ARDS. IGF‐I and PCP‐III levels were analyzed by Spearman Rank Correlation analysis as described in Materials and Methods.
Mentions: IGF‐I has been shown to induce collagen production in fibroblasts in vitro (Goldstein et al. 1989; Olbruck et al. 1998), suggesting one of the consequences of enhanced IGF‐I protein in the lungs of FP‐ARDS patients may be increased collagen deposition in this organ. Therefore, we measured PCP‐III, a byproduct of collagen III processing as a surrogate for collagen deposition in the lungs. Figure 2A shows increased PCP‐III levels in the ELF from FP‐ARDS patients compared to both early ALI/ARDS and controls. Importantly, the protein levels of IGF‐I and PCP‐III in the ELF of FP‐ARDS are significantly correlated versus control individuals (P < 0.01) (Fig. 2B). These results further support the hypothesis that IGF‐I may induce or contribute to collagen production and deposition in the lungs of FP‐ARDS patients.

Bottom Line: Our data show that IGF-I is significantly increased in the ELF in FP-ARDS patients.A significant correlation between IGF-I and PCP-III in the ELF of FP-ARDS patients is found.Our data suggest that IGF-I found in the lungs of FP-ARDS patients results from both increased lung permeability and local production of IGF-I.

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care Medicine, Faculty of Medicine, University of Calgary Health Research Innovation Center, Calgary, Alberta, Canada Immunology Research Group, Faculty of Medicine, University of Calgary Health Research Innovation Center, Calgary, Alberta, Canada.

No MeSH data available.


Related in: MedlinePlus