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Subsarcolemmal lipid droplet responses to a combined endurance and strength exercise intervention.

Li Y, Lee S, Langleite T, Norheim F, Pourteymour S, Jensen J, Stadheim HK, Storås TH, Davanger S, Gulseth HL, Birkeland KI, Drevon CA, Holen T - Physiol Rep (2014)

Bottom Line: Lipid droplet volume in the subsarcolemmal LD population was reduced by ~80%, in both groups, while IMF LD volume was unchanged.Our results show that the SS LD lipid store was sensitive to training, whereas the dominant IMF LD lipid store was not.Thus, net muscle lipid stores can be an insufficient measure for the effects of training.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Institute of Basic Medical Science, University of Oslo, Oslo, Norway.

No MeSH data available.


Related in: MedlinePlus

Lipid droplet diameter in SS, IMF, and lipoautolysosomes. (A) Kernel density plot of LD diameter distribution. Distribution is plotted against LD size (horizontal axis) and probability density (vertical axis). SS LD population distribution is shown before (black line) and after (black stippled line) the intervention. IMF LD population distribution is shown before (gray line) and after (gray stippled line) the intervention. (B) Two lipoautolysosomes with multiple internal LD. Smallest LD are ~80 nm. Also present are one SS LD (lower left) and one IMF LD (lower right). Scale bar is 1 μm.
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fig03: Lipid droplet diameter in SS, IMF, and lipoautolysosomes. (A) Kernel density plot of LD diameter distribution. Distribution is plotted against LD size (horizontal axis) and probability density (vertical axis). SS LD population distribution is shown before (black line) and after (black stippled line) the intervention. IMF LD population distribution is shown before (gray line) and after (gray stippled line) the intervention. (B) Two lipoautolysosomes with multiple internal LD. Smallest LD are ~80 nm. Also present are one SS LD (lower left) and one IMF LD (lower right). Scale bar is 1 μm.

Mentions: Analysis of size distribution of LD revealed a much more narrow distribution of the SS LD diameters after the intervention, losing most of the >1000 nm large diameter LD (Fig. 3A, black line SS LD preintervention, black stippled line SS LD post intervention). LD in the IMF region did not change, although a noticeable double peak before the intervention disappeared (Fig. 3A, gray line IMF LD preintervention, gray stippled line IMF LD post intervention). The double peak could also be observed on the 50‐nm window frequency histograms (Fig. 4).


Subsarcolemmal lipid droplet responses to a combined endurance and strength exercise intervention.

Li Y, Lee S, Langleite T, Norheim F, Pourteymour S, Jensen J, Stadheim HK, Storås TH, Davanger S, Gulseth HL, Birkeland KI, Drevon CA, Holen T - Physiol Rep (2014)

Lipid droplet diameter in SS, IMF, and lipoautolysosomes. (A) Kernel density plot of LD diameter distribution. Distribution is plotted against LD size (horizontal axis) and probability density (vertical axis). SS LD population distribution is shown before (black line) and after (black stippled line) the intervention. IMF LD population distribution is shown before (gray line) and after (gray stippled line) the intervention. (B) Two lipoautolysosomes with multiple internal LD. Smallest LD are ~80 nm. Also present are one SS LD (lower left) and one IMF LD (lower right). Scale bar is 1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4255802&req=5

fig03: Lipid droplet diameter in SS, IMF, and lipoautolysosomes. (A) Kernel density plot of LD diameter distribution. Distribution is plotted against LD size (horizontal axis) and probability density (vertical axis). SS LD population distribution is shown before (black line) and after (black stippled line) the intervention. IMF LD population distribution is shown before (gray line) and after (gray stippled line) the intervention. (B) Two lipoautolysosomes with multiple internal LD. Smallest LD are ~80 nm. Also present are one SS LD (lower left) and one IMF LD (lower right). Scale bar is 1 μm.
Mentions: Analysis of size distribution of LD revealed a much more narrow distribution of the SS LD diameters after the intervention, losing most of the >1000 nm large diameter LD (Fig. 3A, black line SS LD preintervention, black stippled line SS LD post intervention). LD in the IMF region did not change, although a noticeable double peak before the intervention disappeared (Fig. 3A, gray line IMF LD preintervention, gray stippled line IMF LD post intervention). The double peak could also be observed on the 50‐nm window frequency histograms (Fig. 4).

Bottom Line: Lipid droplet volume in the subsarcolemmal LD population was reduced by ~80%, in both groups, while IMF LD volume was unchanged.Our results show that the SS LD lipid store was sensitive to training, whereas the dominant IMF LD lipid store was not.Thus, net muscle lipid stores can be an insufficient measure for the effects of training.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Institute of Basic Medical Science, University of Oslo, Oslo, Norway.

No MeSH data available.


Related in: MedlinePlus