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Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus

SE‐training effects on numbers of myonuclei in atrophied muscles. (A, B) Numbers of myonuclei per myofiber after TS (A) and SE training (B). There were significantly fewer myonuclei per myofiber in the TS group compared with the control non‐TS group, and there were significantly more myonuclei per myofiber in the SE‐trained group compared with the non‐SE‐trained and CON groups. Results are means ± SEM's; n = 6 per group. *P <0.05. (C, D) The numbers of myonuclei per myofiber were counted in different cross sections of soleus muscles: proximal tip–3 mm, 3–6 mm, 6–9 mm, 9–12 mm, and 12 mm–distal tip. Between these different sections, there were no significant differences in the numbers of myonuclei in the CON (B) and SE trained (C) groups. Results are means ± SEM's; n = 3 for each muscle part.
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fig04: SE‐training effects on numbers of myonuclei in atrophied muscles. (A, B) Numbers of myonuclei per myofiber after TS (A) and SE training (B). There were significantly fewer myonuclei per myofiber in the TS group compared with the control non‐TS group, and there were significantly more myonuclei per myofiber in the SE‐trained group compared with the non‐SE‐trained and CON groups. Results are means ± SEM's; n = 6 per group. *P <0.05. (C, D) The numbers of myonuclei per myofiber were counted in different cross sections of soleus muscles: proximal tip–3 mm, 3–6 mm, 6–9 mm, 9–12 mm, and 12 mm–distal tip. Between these different sections, there were no significant differences in the numbers of myonuclei in the CON (B) and SE trained (C) groups. Results are means ± SEM's; n = 3 for each muscle part.

Mentions: A previous study showed that there was a positive correlation between the size of myofibers and the number of myonuclei per myofiber (Roy et al. 1999). Thus, we investigated changes in the number of myonuclei during SE‐training‐facilitated recovery of atrophied muscles. There were significantly fewer myonuclei per myofiber in the TS group compared with the non‐TS group (Fig. 4A). Bruusgaard et al. (2012) reported that the number of myonuclei in the rat soleus muscle did not decrease after 2 weeks of TS, while the myofiber CSA decreased by 34% in rat soleus muscle. However, this study indicated that the myofiber CSA decreased by 54% and the number of myonuclei decreased by 27% in mouse soleus muscle (Figs 2B, 4A), suggesting that the myofiber CSA decreases in earlier phase of atrophy, then the number of myonuclei decreases in advanced atrophy.


Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

SE‐training effects on numbers of myonuclei in atrophied muscles. (A, B) Numbers of myonuclei per myofiber after TS (A) and SE training (B). There were significantly fewer myonuclei per myofiber in the TS group compared with the control non‐TS group, and there were significantly more myonuclei per myofiber in the SE‐trained group compared with the non‐SE‐trained and CON groups. Results are means ± SEM's; n = 6 per group. *P <0.05. (C, D) The numbers of myonuclei per myofiber were counted in different cross sections of soleus muscles: proximal tip–3 mm, 3–6 mm, 6–9 mm, 9–12 mm, and 12 mm–distal tip. Between these different sections, there were no significant differences in the numbers of myonuclei in the CON (B) and SE trained (C) groups. Results are means ± SEM's; n = 3 for each muscle part.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255801&req=5

fig04: SE‐training effects on numbers of myonuclei in atrophied muscles. (A, B) Numbers of myonuclei per myofiber after TS (A) and SE training (B). There were significantly fewer myonuclei per myofiber in the TS group compared with the control non‐TS group, and there were significantly more myonuclei per myofiber in the SE‐trained group compared with the non‐SE‐trained and CON groups. Results are means ± SEM's; n = 6 per group. *P <0.05. (C, D) The numbers of myonuclei per myofiber were counted in different cross sections of soleus muscles: proximal tip–3 mm, 3–6 mm, 6–9 mm, 9–12 mm, and 12 mm–distal tip. Between these different sections, there were no significant differences in the numbers of myonuclei in the CON (B) and SE trained (C) groups. Results are means ± SEM's; n = 3 for each muscle part.
Mentions: A previous study showed that there was a positive correlation between the size of myofibers and the number of myonuclei per myofiber (Roy et al. 1999). Thus, we investigated changes in the number of myonuclei during SE‐training‐facilitated recovery of atrophied muscles. There were significantly fewer myonuclei per myofiber in the TS group compared with the non‐TS group (Fig. 4A). Bruusgaard et al. (2012) reported that the number of myonuclei in the rat soleus muscle did not decrease after 2 weeks of TS, while the myofiber CSA decreased by 34% in rat soleus muscle. However, this study indicated that the myofiber CSA decreased by 54% and the number of myonuclei decreased by 27% in mouse soleus muscle (Figs 2B, 4A), suggesting that the myofiber CSA decreases in earlier phase of atrophy, then the number of myonuclei decreases in advanced atrophy.

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus