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Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus

Changes in muscle histology after stand‐up exercise (SE) training. (A) Photomicrographs of muscle samples (hematoxylin–eosin stained) from mice subjected to TS but not SE training (non‐SE‐trained group), subjected to TS and then SE training (SE‐trained group), and control mice (CON). (B) Whole‐muscle CSA, (C) myofiber CSA, and (D) the number of myofibers for these three groups. Myofiber CSA was significantly greater in the SE‐trained group than in the non‐SE‐trained group and approximately the same as in the CON group. There were no significant differences in the numbers of myofibers among these three groups. Results are means ± SEM's; n = 6 per group. *P <0.05.
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fig03: Changes in muscle histology after stand‐up exercise (SE) training. (A) Photomicrographs of muscle samples (hematoxylin–eosin stained) from mice subjected to TS but not SE training (non‐SE‐trained group), subjected to TS and then SE training (SE‐trained group), and control mice (CON). (B) Whole‐muscle CSA, (C) myofiber CSA, and (D) the number of myofibers for these three groups. Myofiber CSA was significantly greater in the SE‐trained group than in the non‐SE‐trained group and approximately the same as in the CON group. There were no significant differences in the numbers of myofibers among these three groups. Results are means ± SEM's; n = 6 per group. *P <0.05.

Mentions: One group of mice was subjected to SE training for 7 days after their release from TS (SE‐trained group; Fig. 1A). We assessed the effects of SE training on atrophied muscle recovery by evaluating images acquired from soleus muscle transverse sections (Fig. 3A). The average CSA of the whole‐soleus muscle was significantly larger in the SE‐trained group than that in the non‐SE‐trained group, which was subjected to TS but not given exercise cues (Fig. 3B). The average CSA of the whole‐soleus muscle in the SE‐trained group was only slightly smaller than that in the CON group not subjected to TS and SE training, indicating that 7 days of SE training had resulted in nearly complete recovery from soleus muscle disuse atrophy. The whole‐muscle CSA includes the areas of fat, fascia tissues, and interstitial fluid in addition to myofibers. Thus, we measured the CSA of the individual myofibers in order to exclude the area of other tissues.


Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

Changes in muscle histology after stand‐up exercise (SE) training. (A) Photomicrographs of muscle samples (hematoxylin–eosin stained) from mice subjected to TS but not SE training (non‐SE‐trained group), subjected to TS and then SE training (SE‐trained group), and control mice (CON). (B) Whole‐muscle CSA, (C) myofiber CSA, and (D) the number of myofibers for these three groups. Myofiber CSA was significantly greater in the SE‐trained group than in the non‐SE‐trained group and approximately the same as in the CON group. There were no significant differences in the numbers of myofibers among these three groups. Results are means ± SEM's; n = 6 per group. *P <0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4255801&req=5

fig03: Changes in muscle histology after stand‐up exercise (SE) training. (A) Photomicrographs of muscle samples (hematoxylin–eosin stained) from mice subjected to TS but not SE training (non‐SE‐trained group), subjected to TS and then SE training (SE‐trained group), and control mice (CON). (B) Whole‐muscle CSA, (C) myofiber CSA, and (D) the number of myofibers for these three groups. Myofiber CSA was significantly greater in the SE‐trained group than in the non‐SE‐trained group and approximately the same as in the CON group. There were no significant differences in the numbers of myofibers among these three groups. Results are means ± SEM's; n = 6 per group. *P <0.05.
Mentions: One group of mice was subjected to SE training for 7 days after their release from TS (SE‐trained group; Fig. 1A). We assessed the effects of SE training on atrophied muscle recovery by evaluating images acquired from soleus muscle transverse sections (Fig. 3A). The average CSA of the whole‐soleus muscle was significantly larger in the SE‐trained group than that in the non‐SE‐trained group, which was subjected to TS but not given exercise cues (Fig. 3B). The average CSA of the whole‐soleus muscle in the SE‐trained group was only slightly smaller than that in the CON group not subjected to TS and SE training, indicating that 7 days of SE training had resulted in nearly complete recovery from soleus muscle disuse atrophy. The whole‐muscle CSA includes the areas of fat, fascia tissues, and interstitial fluid in addition to myofibers. Thus, we measured the CSA of the individual myofibers in order to exclude the area of other tissues.

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus