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Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus

Scheme for intervention times and experimental methods. (A) Treatment protocols for mouse groups: gray, operant conditioning; black, tail suspension (TS); open, normal housing; black arrowhead, stand‐up exercise (SE) training. (B) During SE training, mice were administered 5‐ethynyl‐2’‐deoxyuridine on day 0, 1, or 2 of the SE‐training period (open arrows), and sacrificed 48 h later. (C) Diagram of the operant‐conditioning device. (D) Learning program for stand‐up training. An electrical shock was generated in a shock grid at 3 sec after displaying light and tone cues. The electrical shock was stopped when a mouse pushed the lever. The electrical shock was not used when a mouse pushed the lever in response to the cues before a shock was generated. The mice acquired the stand‐up exercise without an electrical shock after 7 days of learning (100 times/day).
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fig01: Scheme for intervention times and experimental methods. (A) Treatment protocols for mouse groups: gray, operant conditioning; black, tail suspension (TS); open, normal housing; black arrowhead, stand‐up exercise (SE) training. (B) During SE training, mice were administered 5‐ethynyl‐2’‐deoxyuridine on day 0, 1, or 2 of the SE‐training period (open arrows), and sacrificed 48 h later. (C) Diagram of the operant‐conditioning device. (D) Learning program for stand‐up training. An electrical shock was generated in a shock grid at 3 sec after displaying light and tone cues. The electrical shock was stopped when a mouse pushed the lever. The electrical shock was not used when a mouse pushed the lever in response to the cues before a shock was generated. The mice acquired the stand‐up exercise without an electrical shock after 7 days of learning (100 times/day).

Mentions: To assess the effects of TS on muscle size, we divided 12 pretrained mice into TS and non‐TS groups (n = 6/group; Fig. 1A). Non‐TS mice were housed under the same conditions but were not attached to the TS apparatus. To assess the effects of SE training on atrophied muscles, another 12 TS mice were also divided into two groups (n = 6/group; Fig. 1A); one group was subjected to SE training (Fig. 1A arrowhead) after TS (SE trained group), and the other group was fed normally but not exposed to the cues to elicit SE after TS (non‐SE trained group). Finally, we also included a control (CON) group that was not subjected to TS or SE (n = 6).


Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice.

Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K - Physiol Rep (2014)

Scheme for intervention times and experimental methods. (A) Treatment protocols for mouse groups: gray, operant conditioning; black, tail suspension (TS); open, normal housing; black arrowhead, stand‐up exercise (SE) training. (B) During SE training, mice were administered 5‐ethynyl‐2’‐deoxyuridine on day 0, 1, or 2 of the SE‐training period (open arrows), and sacrificed 48 h later. (C) Diagram of the operant‐conditioning device. (D) Learning program for stand‐up training. An electrical shock was generated in a shock grid at 3 sec after displaying light and tone cues. The electrical shock was stopped when a mouse pushed the lever. The electrical shock was not used when a mouse pushed the lever in response to the cues before a shock was generated. The mice acquired the stand‐up exercise without an electrical shock after 7 days of learning (100 times/day).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255801&req=5

fig01: Scheme for intervention times and experimental methods. (A) Treatment protocols for mouse groups: gray, operant conditioning; black, tail suspension (TS); open, normal housing; black arrowhead, stand‐up exercise (SE) training. (B) During SE training, mice were administered 5‐ethynyl‐2’‐deoxyuridine on day 0, 1, or 2 of the SE‐training period (open arrows), and sacrificed 48 h later. (C) Diagram of the operant‐conditioning device. (D) Learning program for stand‐up training. An electrical shock was generated in a shock grid at 3 sec after displaying light and tone cues. The electrical shock was stopped when a mouse pushed the lever. The electrical shock was not used when a mouse pushed the lever in response to the cues before a shock was generated. The mice acquired the stand‐up exercise without an electrical shock after 7 days of learning (100 times/day).
Mentions: To assess the effects of TS on muscle size, we divided 12 pretrained mice into TS and non‐TS groups (n = 6/group; Fig. 1A). Non‐TS mice were housed under the same conditions but were not attached to the TS apparatus. To assess the effects of SE training on atrophied muscles, another 12 TS mice were also divided into two groups (n = 6/group; Fig. 1A); one group was subjected to SE training (Fig. 1A arrowhead) after TS (SE trained group), and the other group was fed normally but not exposed to the cues to elicit SE after TS (non‐SE trained group). Finally, we also included a control (CON) group that was not subjected to TS or SE (n = 6).

Bottom Line: Seven days after the training, average myofiber cross-sectional area (CSA) of the soleus muscle was significantly greater in the SE-trained group than in the non-SE-trained group (1843 ± 194 μm(2) vs. 1315 ± 153 μm(2)).Mean soleus muscle CSA in the SE trained group was not different from that in the CON group subjected to neither TS nor SE training (2005 ± 196 μm(2)), indicating that SE training caused nearly complete recovery from muscle atrophy.The number of myonuclei per myofiber was increased by ~60% in the SE-trained group compared with the non-SE-trained and CON groups (0.92 ± 0.03 vs. 0.57 ± 0.03 and 0.56 ± 0.11, respectively).

View Article: PubMed Central - PubMed

Affiliation: Physical and Occupational Therapy Program, Nagoya University Graduate School of Medicine, Nagoya, Japan Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan.

No MeSH data available.


Related in: MedlinePlus