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MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat portal fibroblasts.

Hickman DA, Syal G, Fausther M, Lavoie EG, Goree JR, Storrie B, Dranoff JA - Physiol Rep (2014)

Bottom Line: We examined the effect of MCP-1 on release of matrix metalloproteinase-9 (MMP-9) by rat PF.We found that MCP-1 blocks PF release of MMP-9 in a posttranslational fashion.Our data demonstrated that, in the presence of MCP-1, MMP-9-containing vesicles were shunted to a lysosome-like compartment.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

No MeSH data available.


Related in: MedlinePlus

MCP‐1 causes MMP‐9‐CFP to be trafficked into lysosomes in live PF. PF were transfected with CFP vector alone (as a transfection control) or MMP‐9‐CFP. CFP fluorescence is pseudocolored green. Cells were incubated with Lysotracker Red (pseudocolored red) to visualize lysosomal membrane structures. No colocalization of CFP and Lysotracker was noted in cells treated with either MCP‐1 or MCP‐1 + blocking antibody (bAb). No colocalization of MMP‐9‐CFP with Lysotracker Red was seen in cells treated with MCP‐1 + bAb; however, strong colocalization MMP‐9‐CFP with Lysotracker Red was seen after MCP‐1 (10 ng/mL) treatment.
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fig04: MCP‐1 causes MMP‐9‐CFP to be trafficked into lysosomes in live PF. PF were transfected with CFP vector alone (as a transfection control) or MMP‐9‐CFP. CFP fluorescence is pseudocolored green. Cells were incubated with Lysotracker Red (pseudocolored red) to visualize lysosomal membrane structures. No colocalization of CFP and Lysotracker was noted in cells treated with either MCP‐1 or MCP‐1 + blocking antibody (bAb). No colocalization of MMP‐9‐CFP with Lysotracker Red was seen in cells treated with MCP‐1 + bAb; however, strong colocalization MMP‐9‐CFP with Lysotracker Red was seen after MCP‐1 (10 ng/mL) treatment.

Mentions: The effect of MCP‐1 in the presence or absence of MCP‐1 blocking antibody (bAb) (Kruglov et al. 2006) was assessed in live PF transfected with MMP‐9‐CFP and loaded with Lysotracker Red. Control cells were transfected with CFP alone. As seen in Figure 4, PF treated with MCP‐1 + bAb demonstrate MMP‐9‐CFP fluorescence in the perinuclear cytoplasm, but MMP‐9‐CFP fluorescence does not colocalize with Lysotracker Red fluorescence. In contrast, PF treated with MCP‐1 demonstrate MMP‐9‐CFP fluorescence in clusters within the cytoplasm that colocalize with Lysotracker Red. Taken together, this finding suggested that MCP‐1 induces trafficking of MMP‐9‐CFP to lysosomes.


MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat portal fibroblasts.

Hickman DA, Syal G, Fausther M, Lavoie EG, Goree JR, Storrie B, Dranoff JA - Physiol Rep (2014)

MCP‐1 causes MMP‐9‐CFP to be trafficked into lysosomes in live PF. PF were transfected with CFP vector alone (as a transfection control) or MMP‐9‐CFP. CFP fluorescence is pseudocolored green. Cells were incubated with Lysotracker Red (pseudocolored red) to visualize lysosomal membrane structures. No colocalization of CFP and Lysotracker was noted in cells treated with either MCP‐1 or MCP‐1 + blocking antibody (bAb). No colocalization of MMP‐9‐CFP with Lysotracker Red was seen in cells treated with MCP‐1 + bAb; however, strong colocalization MMP‐9‐CFP with Lysotracker Red was seen after MCP‐1 (10 ng/mL) treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255798&req=5

fig04: MCP‐1 causes MMP‐9‐CFP to be trafficked into lysosomes in live PF. PF were transfected with CFP vector alone (as a transfection control) or MMP‐9‐CFP. CFP fluorescence is pseudocolored green. Cells were incubated with Lysotracker Red (pseudocolored red) to visualize lysosomal membrane structures. No colocalization of CFP and Lysotracker was noted in cells treated with either MCP‐1 or MCP‐1 + blocking antibody (bAb). No colocalization of MMP‐9‐CFP with Lysotracker Red was seen in cells treated with MCP‐1 + bAb; however, strong colocalization MMP‐9‐CFP with Lysotracker Red was seen after MCP‐1 (10 ng/mL) treatment.
Mentions: The effect of MCP‐1 in the presence or absence of MCP‐1 blocking antibody (bAb) (Kruglov et al. 2006) was assessed in live PF transfected with MMP‐9‐CFP and loaded with Lysotracker Red. Control cells were transfected with CFP alone. As seen in Figure 4, PF treated with MCP‐1 + bAb demonstrate MMP‐9‐CFP fluorescence in the perinuclear cytoplasm, but MMP‐9‐CFP fluorescence does not colocalize with Lysotracker Red fluorescence. In contrast, PF treated with MCP‐1 demonstrate MMP‐9‐CFP fluorescence in clusters within the cytoplasm that colocalize with Lysotracker Red. Taken together, this finding suggested that MCP‐1 induces trafficking of MMP‐9‐CFP to lysosomes.

Bottom Line: We examined the effect of MCP-1 on release of matrix metalloproteinase-9 (MMP-9) by rat PF.We found that MCP-1 blocks PF release of MMP-9 in a posttranslational fashion.Our data demonstrated that, in the presence of MCP-1, MMP-9-containing vesicles were shunted to a lysosome-like compartment.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

No MeSH data available.


Related in: MedlinePlus