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MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat portal fibroblasts.

Hickman DA, Syal G, Fausther M, Lavoie EG, Goree JR, Storrie B, Dranoff JA - Physiol Rep (2014)

Bottom Line: We examined the effect of MCP-1 on release of matrix metalloproteinase-9 (MMP-9) by rat PF.We found that MCP-1 blocks PF release of MMP-9 in a posttranslational fashion.Our data demonstrated that, in the presence of MCP-1, MMP-9-containing vesicles were shunted to a lysosome-like compartment.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

No MeSH data available.


Related in: MedlinePlus

MCP‐1 causes the formation of intracellular MMP‐9 clusters in PF. Representative confocal micrographs are shown. PF were plated on glass coverslips 1 day after isolation and treated overnight with buffer alone or MCP‐1 (10 ng/mL). MMP‐9 immunostaining is pseudocolored green, rhodamine phalloidin staining (F‐actin) is pseudolored red, and TOPRO‐3 staining (nuclei) is pseudocolored blue. Scant MMP‐9 can be detected in untreated cells; however, increased numbers of large clusters of MMP‐9 fluorescence can be seen in PF treated with MCP‐1. Insets are digitally enhanced at 2X.
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fig03: MCP‐1 causes the formation of intracellular MMP‐9 clusters in PF. Representative confocal micrographs are shown. PF were plated on glass coverslips 1 day after isolation and treated overnight with buffer alone or MCP‐1 (10 ng/mL). MMP‐9 immunostaining is pseudocolored green, rhodamine phalloidin staining (F‐actin) is pseudolored red, and TOPRO‐3 staining (nuclei) is pseudocolored blue. Scant MMP‐9 can be detected in untreated cells; however, increased numbers of large clusters of MMP‐9 fluorescence can be seen in PF treated with MCP‐1. Insets are digitally enhanced at 2X.

Mentions: We investigated the effect of MCP‐1 on MMP‐9 localization within PF via confocal immunofluorescence (Fig. 3). No MMP‐9 protein could be detected in cells treated with buffer alone. In contrast, PF treated with MCP‐1 exhibited large clusters of MMP‐9 localized to the perinuclear cytoplasm, supporting the concept that MCP‐1 may block MMP‐9 protein release.


MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat portal fibroblasts.

Hickman DA, Syal G, Fausther M, Lavoie EG, Goree JR, Storrie B, Dranoff JA - Physiol Rep (2014)

MCP‐1 causes the formation of intracellular MMP‐9 clusters in PF. Representative confocal micrographs are shown. PF were plated on glass coverslips 1 day after isolation and treated overnight with buffer alone or MCP‐1 (10 ng/mL). MMP‐9 immunostaining is pseudocolored green, rhodamine phalloidin staining (F‐actin) is pseudolored red, and TOPRO‐3 staining (nuclei) is pseudocolored blue. Scant MMP‐9 can be detected in untreated cells; however, increased numbers of large clusters of MMP‐9 fluorescence can be seen in PF treated with MCP‐1. Insets are digitally enhanced at 2X.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255798&req=5

fig03: MCP‐1 causes the formation of intracellular MMP‐9 clusters in PF. Representative confocal micrographs are shown. PF were plated on glass coverslips 1 day after isolation and treated overnight with buffer alone or MCP‐1 (10 ng/mL). MMP‐9 immunostaining is pseudocolored green, rhodamine phalloidin staining (F‐actin) is pseudolored red, and TOPRO‐3 staining (nuclei) is pseudocolored blue. Scant MMP‐9 can be detected in untreated cells; however, increased numbers of large clusters of MMP‐9 fluorescence can be seen in PF treated with MCP‐1. Insets are digitally enhanced at 2X.
Mentions: We investigated the effect of MCP‐1 on MMP‐9 localization within PF via confocal immunofluorescence (Fig. 3). No MMP‐9 protein could be detected in cells treated with buffer alone. In contrast, PF treated with MCP‐1 exhibited large clusters of MMP‐9 localized to the perinuclear cytoplasm, supporting the concept that MCP‐1 may block MMP‐9 protein release.

Bottom Line: We examined the effect of MCP-1 on release of matrix metalloproteinase-9 (MMP-9) by rat PF.We found that MCP-1 blocks PF release of MMP-9 in a posttranslational fashion.Our data demonstrated that, in the presence of MCP-1, MMP-9-containing vesicles were shunted to a lysosome-like compartment.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

No MeSH data available.


Related in: MedlinePlus