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Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain.

Isasawin S, Aketarawong N, Lertsiri S, Thanaphum S - BMC Genet. (2014)

Bottom Line: This fruit fly belongs to Bactrocera dorsalis species complex.Further experiments showed that the sterile males of Salaya5 can compete with wild males for mating with wild females in field cage conditions.In addition, mating competitiveness tests suggested that Salaya5 has a potential to be used in B. carambolae SIT programs based on male-only releases.

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ABSTRACT

Background: The carambola fruit fly, Bactrocera carambolae Drew & Hancock is a high profile key pest that is widely distributed in the southwestern ASEAN region. In addition, it has trans-continentally invaded Suriname, where it has been expanding east and southward since 1975. This fruit fly belongs to Bactrocera dorsalis species complex. The development and application of a genetic sexing strain (Salaya1) of B. dorsalis sensu stricto (s.s.) (Hendel) for the sterile insect technique (SIT) has improved the fruit fly control. However, matings between B. dorsalis s.s. and B. carambolae are incompatible, which hinder the application of the Salaya1 strain to control the carambola fruit fly. To solve this problem, we introduced genetic sexing components from the Salaya1 strain into the B. carambolae genome by interspecific hybridization.

Results: Morphological characteristics, mating competitiveness, male pheromone profiles, and genetic relationships revealed consistencies that helped to distinguish Salaya1 and B. carambolae strains. A Y-autosome translocation linking the dominant wild-type allele of white pupae gene and a free autosome carrying a recessive white pupae homologue from the Salaya1 strain were introgressed into the gene pool of B. carambolae. A panel of Y-pseudo-linked microsatellite loci of the Salaya1 strain served as markers for the introgression experiments. This resulted in a newly derived genetic sexing strain called Salaya5, with morphological characteristics corresponding to B. carambolae. The rectal gland pheromone profile of Salaya5 males also contained a distinctive component of B. carambolae. Microsatellite DNA analyses confirmed the close genetic relationships between the Salaya5 strain and wild B. carambolae populations. Further experiments showed that the sterile males of Salaya5 can compete with wild males for mating with wild females in field cage conditions.

Conclusions: Introgression of sex sorting components from the Salaya1 strain to a closely related B. carambolae strain generated a new genetic sexing strain, Salaya5. Morphology-based taxonomic characteristics, distinctive pheromone components, microsatellite DNA markers, genetic relationships, and mating competitiveness provided parental baseline data and validation tools for the new strain. The Salaya5 strain shows a close similarity with those features in the wild B. carambolae strain. In addition, mating competitiveness tests suggested that Salaya5 has a potential to be used in B. carambolae SIT programs based on male-only releases.

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Gas chromatograms of pheromone profile from male rectal glands of B. dorsalis and B. carambolae. B. dorsalis (Salaya1) (A), B. carambolae (Jakarta) (B), and Salaya5 (C), after methyl eugenol consumption, tentatively identified as (1) 4,5-dimethoxy-2-(2-propenyl)phenol, (2) (E)-coniferyl alcohol, and (3) 6-oxo-1-nonanol.
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Figure 2: Gas chromatograms of pheromone profile from male rectal glands of B. dorsalis and B. carambolae. B. dorsalis (Salaya1) (A), B. carambolae (Jakarta) (B), and Salaya5 (C), after methyl eugenol consumption, tentatively identified as (1) 4,5-dimethoxy-2-(2-propenyl)phenol, (2) (E)-coniferyl alcohol, and (3) 6-oxo-1-nonanol.

Mentions: The other type of distinctive chemical characters between B. dorsalis and B. carambolae is the volatile components of the methyl eugenol (ME) fed male rectal glands [15,16]. Each of the rectal glands of the B. dorsalis (Salaya1 strain) males after ME consumption contained a distinctive 4, 5-dimethoxy-2-(2-propenyl) phenol (DMP) and non-distinctive (E)-coniferyl alcohol (CF), whereas only CF was detected along with a distinctive major endogenous rectal gland component, 6-oxo-1-nonanol (OXO), in that of individual wild B. carambolae males (Figures 2A and 2B). These rectal gland male pheromone profiles can consistently differentiate the Salaya1 strain and the B. carambolae according to chemotaxonomic references [16,20].


Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain.

Isasawin S, Aketarawong N, Lertsiri S, Thanaphum S - BMC Genet. (2014)

Gas chromatograms of pheromone profile from male rectal glands of B. dorsalis and B. carambolae. B. dorsalis (Salaya1) (A), B. carambolae (Jakarta) (B), and Salaya5 (C), after methyl eugenol consumption, tentatively identified as (1) 4,5-dimethoxy-2-(2-propenyl)phenol, (2) (E)-coniferyl alcohol, and (3) 6-oxo-1-nonanol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255791&req=5

Figure 2: Gas chromatograms of pheromone profile from male rectal glands of B. dorsalis and B. carambolae. B. dorsalis (Salaya1) (A), B. carambolae (Jakarta) (B), and Salaya5 (C), after methyl eugenol consumption, tentatively identified as (1) 4,5-dimethoxy-2-(2-propenyl)phenol, (2) (E)-coniferyl alcohol, and (3) 6-oxo-1-nonanol.
Mentions: The other type of distinctive chemical characters between B. dorsalis and B. carambolae is the volatile components of the methyl eugenol (ME) fed male rectal glands [15,16]. Each of the rectal glands of the B. dorsalis (Salaya1 strain) males after ME consumption contained a distinctive 4, 5-dimethoxy-2-(2-propenyl) phenol (DMP) and non-distinctive (E)-coniferyl alcohol (CF), whereas only CF was detected along with a distinctive major endogenous rectal gland component, 6-oxo-1-nonanol (OXO), in that of individual wild B. carambolae males (Figures 2A and 2B). These rectal gland male pheromone profiles can consistently differentiate the Salaya1 strain and the B. carambolae according to chemotaxonomic references [16,20].

Bottom Line: This fruit fly belongs to Bactrocera dorsalis species complex.Further experiments showed that the sterile males of Salaya5 can compete with wild males for mating with wild females in field cage conditions.In addition, mating competitiveness tests suggested that Salaya5 has a potential to be used in B. carambolae SIT programs based on male-only releases.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: The carambola fruit fly, Bactrocera carambolae Drew & Hancock is a high profile key pest that is widely distributed in the southwestern ASEAN region. In addition, it has trans-continentally invaded Suriname, where it has been expanding east and southward since 1975. This fruit fly belongs to Bactrocera dorsalis species complex. The development and application of a genetic sexing strain (Salaya1) of B. dorsalis sensu stricto (s.s.) (Hendel) for the sterile insect technique (SIT) has improved the fruit fly control. However, matings between B. dorsalis s.s. and B. carambolae are incompatible, which hinder the application of the Salaya1 strain to control the carambola fruit fly. To solve this problem, we introduced genetic sexing components from the Salaya1 strain into the B. carambolae genome by interspecific hybridization.

Results: Morphological characteristics, mating competitiveness, male pheromone profiles, and genetic relationships revealed consistencies that helped to distinguish Salaya1 and B. carambolae strains. A Y-autosome translocation linking the dominant wild-type allele of white pupae gene and a free autosome carrying a recessive white pupae homologue from the Salaya1 strain were introgressed into the gene pool of B. carambolae. A panel of Y-pseudo-linked microsatellite loci of the Salaya1 strain served as markers for the introgression experiments. This resulted in a newly derived genetic sexing strain called Salaya5, with morphological characteristics corresponding to B. carambolae. The rectal gland pheromone profile of Salaya5 males also contained a distinctive component of B. carambolae. Microsatellite DNA analyses confirmed the close genetic relationships between the Salaya5 strain and wild B. carambolae populations. Further experiments showed that the sterile males of Salaya5 can compete with wild males for mating with wild females in field cage conditions.

Conclusions: Introgression of sex sorting components from the Salaya1 strain to a closely related B. carambolae strain generated a new genetic sexing strain, Salaya5. Morphology-based taxonomic characteristics, distinctive pheromone components, microsatellite DNA markers, genetic relationships, and mating competitiveness provided parental baseline data and validation tools for the new strain. The Salaya5 strain shows a close similarity with those features in the wild B. carambolae strain. In addition, mating competitiveness tests suggested that Salaya5 has a potential to be used in B. carambolae SIT programs based on male-only releases.

Show MeSH
Related in: MedlinePlus