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The Bactrocera dorsalis species complex: comparative cytogenetic analysis in support of Sterile Insect Technique applications.

Augustinos AA, Drosopoulou E, Gariou-Papalexiou A, Bourtzis K, Mavragani-Tsipidou P, Zacharopoulou A - BMC Genet. (2014)

Bottom Line: Therefore, the use of the available polytene chromosome maps for B. dorsalis s.s. as reference maps for all these five biological entities is proposed.Present data provide important insight in the genetic relationships among the different members of the B. dorsalis complex, and, along with other studies in the field, can facilitate SIT applications targeting this complex.Moreover, the availability of 'universal' reference polytene chromosome maps for members of the complex, along with the documented application of in situ hybridization, can facilitate ongoing and future genome projects in this complex.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: The Bactrocera dorsalis species complex currently harbors approximately 90 different members. The species complex has undergone many revisions in the past decades, and there is still an ongoing debate about the species limits. The availability of a variety of tools and approaches, such as molecular-genomic and cytogenetic analyses, are expected to shed light on the rather complicated issues of species complexes and incipient speciation. The clarification of genetic relationships among the different members of this complex is a prerequisite for the rational application of sterile insect technique (SIT) approaches for population control.

Results: Colonies established in the Insect Pest Control Laboratory (IPCL) (Seibersdorf, Vienna), representing five of the main economic important members of the Bactrocera dorsalis complex were cytologically characterized. The taxa under study were B. dorsalis s.s., B. philippinensis, B. papayae, B. invadens and B. carambolae. Mitotic and polytene chromosome analyses did not reveal any chromosomal characteristics that could be used to distinguish between the investigated members of the B. dorsalis complex. Therefore, their polytene chromosomes can be regarded as homosequential with the reference maps of B. dorsalis s.s.. In situ hybridization of six genes further supported the proposed homosequentiallity of the chromosomes of these specific members of the complex.

Conclusions: The present analysis supports that the polytene chromosomes of the five taxa under study are homosequential. Therefore, the use of the available polytene chromosome maps for B. dorsalis s.s. as reference maps for all these five biological entities is proposed. Present data provide important insight in the genetic relationships among the different members of the B. dorsalis complex, and, along with other studies in the field, can facilitate SIT applications targeting this complex. Moreover, the availability of 'universal' reference polytene chromosome maps for members of the complex, along with the documented application of in situ hybridization, can facilitate ongoing and future genome projects in this complex.

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Comparison of chromosome arm 2R between B. dorsalis s.s. and B. tryoni. a) Chromosome arm 2R of B. dorsalis s.s. and b) chromosome arm 2R of B. tryoni. Note the fixed inversion between the two species.
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Figure 7: Comparison of chromosome arm 2R between B. dorsalis s.s. and B. tryoni. a) Chromosome arm 2R of B. dorsalis s.s. and b) chromosome arm 2R of B. tryoni. Note the fixed inversion between the two species.

Mentions: In tephritid flies, genomic data are still scarce, and polytene chromosome maps are restricted to a few species. However, comparative polytene chromosome analysis and in situ mapping of unique genes show that chromosomal rearrangements characterize different species [50,51,53,58,69], suggesting their possible involvement in speciation. A comparative analysis of polytene chromosome maps of B. dorsalis s.s. and B. tryoni, a species outside, but closely related to the B. dorsalis complex, clearly shows at least one fixed inversion in polytene arm 2R that differentiates the two species (Figure 7).


The Bactrocera dorsalis species complex: comparative cytogenetic analysis in support of Sterile Insect Technique applications.

Augustinos AA, Drosopoulou E, Gariou-Papalexiou A, Bourtzis K, Mavragani-Tsipidou P, Zacharopoulou A - BMC Genet. (2014)

Comparison of chromosome arm 2R between B. dorsalis s.s. and B. tryoni. a) Chromosome arm 2R of B. dorsalis s.s. and b) chromosome arm 2R of B. tryoni. Note the fixed inversion between the two species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255788&req=5

Figure 7: Comparison of chromosome arm 2R between B. dorsalis s.s. and B. tryoni. a) Chromosome arm 2R of B. dorsalis s.s. and b) chromosome arm 2R of B. tryoni. Note the fixed inversion between the two species.
Mentions: In tephritid flies, genomic data are still scarce, and polytene chromosome maps are restricted to a few species. However, comparative polytene chromosome analysis and in situ mapping of unique genes show that chromosomal rearrangements characterize different species [50,51,53,58,69], suggesting their possible involvement in speciation. A comparative analysis of polytene chromosome maps of B. dorsalis s.s. and B. tryoni, a species outside, but closely related to the B. dorsalis complex, clearly shows at least one fixed inversion in polytene arm 2R that differentiates the two species (Figure 7).

Bottom Line: Therefore, the use of the available polytene chromosome maps for B. dorsalis s.s. as reference maps for all these five biological entities is proposed.Present data provide important insight in the genetic relationships among the different members of the B. dorsalis complex, and, along with other studies in the field, can facilitate SIT applications targeting this complex.Moreover, the availability of 'universal' reference polytene chromosome maps for members of the complex, along with the documented application of in situ hybridization, can facilitate ongoing and future genome projects in this complex.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: The Bactrocera dorsalis species complex currently harbors approximately 90 different members. The species complex has undergone many revisions in the past decades, and there is still an ongoing debate about the species limits. The availability of a variety of tools and approaches, such as molecular-genomic and cytogenetic analyses, are expected to shed light on the rather complicated issues of species complexes and incipient speciation. The clarification of genetic relationships among the different members of this complex is a prerequisite for the rational application of sterile insect technique (SIT) approaches for population control.

Results: Colonies established in the Insect Pest Control Laboratory (IPCL) (Seibersdorf, Vienna), representing five of the main economic important members of the Bactrocera dorsalis complex were cytologically characterized. The taxa under study were B. dorsalis s.s., B. philippinensis, B. papayae, B. invadens and B. carambolae. Mitotic and polytene chromosome analyses did not reveal any chromosomal characteristics that could be used to distinguish between the investigated members of the B. dorsalis complex. Therefore, their polytene chromosomes can be regarded as homosequential with the reference maps of B. dorsalis s.s.. In situ hybridization of six genes further supported the proposed homosequentiallity of the chromosomes of these specific members of the complex.

Conclusions: The present analysis supports that the polytene chromosomes of the five taxa under study are homosequential. Therefore, the use of the available polytene chromosome maps for B. dorsalis s.s. as reference maps for all these five biological entities is proposed. Present data provide important insight in the genetic relationships among the different members of the B. dorsalis complex, and, along with other studies in the field, can facilitate SIT applications targeting this complex. Moreover, the availability of 'universal' reference polytene chromosome maps for members of the complex, along with the documented application of in situ hybridization, can facilitate ongoing and future genome projects in this complex.

Show MeSH
Related in: MedlinePlus