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Dynamics of genetic variability in Anastrepha fraterculus (Diptera: Tephritidae) during adaptation to laboratory rearing conditions.

Parreño MA, Scannapieco AC, Remis MI, Juri M, Vera MT, Segura DF, Cladera JL, Lanzavecchia SB - BMC Genet. (2014)

Bottom Line: A better understanding of the genetic variability during the introduction and adaptation of wild A. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful Sterile Insect Technique (SIT) efforts.In CL, the relatively high values of genetic variability appear to be maintained across generations and could denote an intrinsic capacity to avoid the loss of genetic diversity in time.The impact of evolutionary forces on this species during the adaptation process as well as the best approach to choose strategies to introduce experimental and mass-reared A. fraterculus strains for SIT programs are discussed.

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ABSTRACT

Background: Anastrepha fraterculus is one of the most important fruit fly plagues in the American continent and only chemical control is applied in the field to diminish its population densities. A better understanding of the genetic variability during the introduction and adaptation of wild A. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful Sterile Insect Technique (SIT) efforts.

Methods: The present study aims to analyze the dynamics of changes in genetic variability during the first six generations under artificial rearing conditions in two populations: a) a wild population recently introduced to laboratory culture, named TW and, b) a long-established control line, named CL.

Results: Results showed a declining tendency of genetic variability in TW. In CL, the relatively high values of genetic variability appear to be maintained across generations and could denote an intrinsic capacity to avoid the loss of genetic diversity in time.

Discussion: The impact of evolutionary forces on this species during the adaptation process as well as the best approach to choose strategies to introduce experimental and mass-reared A. fraterculus strains for SIT programs are discussed.

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Mean allelic richness, AR (A), and mean expected heterozygosity, He (B), at generations n, n+3 and n+6 under laboratory conditions of rearing for the four populations of A. fraterculus.
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Figure 1: Mean allelic richness, AR (A), and mean expected heterozygosity, He (B), at generations n, n+3 and n+6 under laboratory conditions of rearing for the four populations of A. fraterculus.

Mentions: The two lines analyzed here, the long-established CL and the recently introduced TW, showed genetic heterogeneity. The AMOVA performed in Gn detected significant differences between the lines (F = 0.0196; P = 0.00098), with 2% of genetic variability explained by this source of variation. The results from the Exact G test considering all loci in this generation also showed that genotypic frequencies differ between the lines (P<0.05). In addition, the genetic variability parameters showed high initial values for these lines. Particularly, TW seemed to show higher diversity in terms of mean number of alleles per locus (Na), allelic richness (AR) and expected heterozygosity (He) than CL in the parental generation Gn (Table 1; Figure 1A,B), with marginally significant differences for AR between lines (Z = 1,72; P = 0.08, Wilcoxon Matched Pairs Test).


Dynamics of genetic variability in Anastrepha fraterculus (Diptera: Tephritidae) during adaptation to laboratory rearing conditions.

Parreño MA, Scannapieco AC, Remis MI, Juri M, Vera MT, Segura DF, Cladera JL, Lanzavecchia SB - BMC Genet. (2014)

Mean allelic richness, AR (A), and mean expected heterozygosity, He (B), at generations n, n+3 and n+6 under laboratory conditions of rearing for the four populations of A. fraterculus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255785&req=5

Figure 1: Mean allelic richness, AR (A), and mean expected heterozygosity, He (B), at generations n, n+3 and n+6 under laboratory conditions of rearing for the four populations of A. fraterculus.
Mentions: The two lines analyzed here, the long-established CL and the recently introduced TW, showed genetic heterogeneity. The AMOVA performed in Gn detected significant differences between the lines (F = 0.0196; P = 0.00098), with 2% of genetic variability explained by this source of variation. The results from the Exact G test considering all loci in this generation also showed that genotypic frequencies differ between the lines (P<0.05). In addition, the genetic variability parameters showed high initial values for these lines. Particularly, TW seemed to show higher diversity in terms of mean number of alleles per locus (Na), allelic richness (AR) and expected heterozygosity (He) than CL in the parental generation Gn (Table 1; Figure 1A,B), with marginally significant differences for AR between lines (Z = 1,72; P = 0.08, Wilcoxon Matched Pairs Test).

Bottom Line: A better understanding of the genetic variability during the introduction and adaptation of wild A. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful Sterile Insect Technique (SIT) efforts.In CL, the relatively high values of genetic variability appear to be maintained across generations and could denote an intrinsic capacity to avoid the loss of genetic diversity in time.The impact of evolutionary forces on this species during the adaptation process as well as the best approach to choose strategies to introduce experimental and mass-reared A. fraterculus strains for SIT programs are discussed.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Anastrepha fraterculus is one of the most important fruit fly plagues in the American continent and only chemical control is applied in the field to diminish its population densities. A better understanding of the genetic variability during the introduction and adaptation of wild A. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful Sterile Insect Technique (SIT) efforts.

Methods: The present study aims to analyze the dynamics of changes in genetic variability during the first six generations under artificial rearing conditions in two populations: a) a wild population recently introduced to laboratory culture, named TW and, b) a long-established control line, named CL.

Results: Results showed a declining tendency of genetic variability in TW. In CL, the relatively high values of genetic variability appear to be maintained across generations and could denote an intrinsic capacity to avoid the loss of genetic diversity in time.

Discussion: The impact of evolutionary forces on this species during the adaptation process as well as the best approach to choose strategies to introduce experimental and mass-reared A. fraterculus strains for SIT programs are discussed.

Show MeSH
Related in: MedlinePlus