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Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus Hirsutella minnesotensis.

Lai Y, Liu K, Zhang X, Zhang X, Li K, Wang N, Shu C, Wu Y, Wang C, Bushley KE, Xiang M, Liu X - Genome Biol Evol (2014)

Bottom Line: Its genome was de novo sequenced and compared with five entomopathogenic fungi in the Hypocreales and three nematode-trapping fungi in the Orbiliales (Ascomycota).Those results indicate that H. minnesotensis has evolved different mechanism for nematode endoparasitism compared with nematode-trapping fungi.Genome and transcriptome analyses provided comprehensive understanding of the evolution and lifestyle of nematode endoparasitism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.

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Phylogeny and genome features of Hirsutella minnesotensis and other 14 sequenced Ascomycota genomes. (A) Genome-based phylogenetic tree of 15 Ascomycota species computed using 898 single-copy orthologs. Bootstrap values are indicated beside the nodes. Life strategies and host preference (green box for nematode-trapping fungi, pink box for nematode endoparasitic fungus, and blue box for insect fungi) are indicated. (B) Number of predicted genes and gene conservation among 15 Ascomycota species.
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evu241-F2: Phylogeny and genome features of Hirsutella minnesotensis and other 14 sequenced Ascomycota genomes. (A) Genome-based phylogenetic tree of 15 Ascomycota species computed using 898 single-copy orthologs. Bootstrap values are indicated beside the nodes. Life strategies and host preference (green box for nematode-trapping fungi, pink box for nematode endoparasitic fungus, and blue box for insect fungi) are indicated. (B) Number of predicted genes and gene conservation among 15 Ascomycota species.

Mentions: Compared with 14 other sequenced Ascomycota genomes, most orthologous genes (10,570) in H. minnesotensis are shared, whereas 2,132 genes are completely unique for this fungus (fig. 2). Despite their conservative relationships, 66.9% of the species-specific genes in H. minnesotensis do not contain recognizable Pfam domains (supplementary table S5, Supplementary Material online), indicating their unique gene functions. Of the remaining with Pfam domains, the major functional categories included TEs, secondary metabolism, and transcription factors (supplementary table S5, Supplementary Material online), which were also identified as significantly enriched domains in the fungus (Fisher’s exact test, P < 0.05).Fig. 2.—


Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus Hirsutella minnesotensis.

Lai Y, Liu K, Zhang X, Zhang X, Li K, Wang N, Shu C, Wu Y, Wang C, Bushley KE, Xiang M, Liu X - Genome Biol Evol (2014)

Phylogeny and genome features of Hirsutella minnesotensis and other 14 sequenced Ascomycota genomes. (A) Genome-based phylogenetic tree of 15 Ascomycota species computed using 898 single-copy orthologs. Bootstrap values are indicated beside the nodes. Life strategies and host preference (green box for nematode-trapping fungi, pink box for nematode endoparasitic fungus, and blue box for insect fungi) are indicated. (B) Number of predicted genes and gene conservation among 15 Ascomycota species.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255773&req=5

evu241-F2: Phylogeny and genome features of Hirsutella minnesotensis and other 14 sequenced Ascomycota genomes. (A) Genome-based phylogenetic tree of 15 Ascomycota species computed using 898 single-copy orthologs. Bootstrap values are indicated beside the nodes. Life strategies and host preference (green box for nematode-trapping fungi, pink box for nematode endoparasitic fungus, and blue box for insect fungi) are indicated. (B) Number of predicted genes and gene conservation among 15 Ascomycota species.
Mentions: Compared with 14 other sequenced Ascomycota genomes, most orthologous genes (10,570) in H. minnesotensis are shared, whereas 2,132 genes are completely unique for this fungus (fig. 2). Despite their conservative relationships, 66.9% of the species-specific genes in H. minnesotensis do not contain recognizable Pfam domains (supplementary table S5, Supplementary Material online), indicating their unique gene functions. Of the remaining with Pfam domains, the major functional categories included TEs, secondary metabolism, and transcription factors (supplementary table S5, Supplementary Material online), which were also identified as significantly enriched domains in the fungus (Fisher’s exact test, P < 0.05).Fig. 2.—

Bottom Line: Its genome was de novo sequenced and compared with five entomopathogenic fungi in the Hypocreales and three nematode-trapping fungi in the Orbiliales (Ascomycota).Those results indicate that H. minnesotensis has evolved different mechanism for nematode endoparasitism compared with nematode-trapping fungi.Genome and transcriptome analyses provided comprehensive understanding of the evolution and lifestyle of nematode endoparasitism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.

Show MeSH
Related in: MedlinePlus