Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus Hirsutella minnesotensis.
Bottom Line: Its genome was de novo sequenced and compared with five entomopathogenic fungi in the Hypocreales and three nematode-trapping fungi in the Orbiliales (Ascomycota).Those results indicate that H. minnesotensis has evolved different mechanism for nematode endoparasitism compared with nematode-trapping fungi.Genome and transcriptome analyses provided comprehensive understanding of the evolution and lifestyle of nematode endoparasitism.
Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.Show MeSH
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Mentions: Compared with 14 other sequenced Ascomycota genomes, most orthologous genes (10,570) in H. minnesotensis are shared, whereas 2,132 genes are completely unique for this fungus (fig. 2). Despite their conservative relationships, 66.9% of the species-specific genes in H. minnesotensis do not contain recognizable Pfam domains (supplementary table S5, Supplementary Material online), indicating their unique gene functions. Of the remaining with Pfam domains, the major functional categories included TEs, secondary metabolism, and transcription factors (supplementary table S5, Supplementary Material online), which were also identified as significantly enriched domains in the fungus (Fisher’s exact test, P < 0.05).Fig. 2.—
Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.