Extensive pericentric rearrangements in the bread wheat (Triticum aestivum L.) genotype "Chinese Spring" revealed from chromosome shotgun sequence data.
Bottom Line: Based on patterns of their homoeologous arm locations, 551 genes indicated the presence of pericentric inversions in at least 10 of the 21 chromosomes.Available data from deletion bin-mapped expressed sequence tags and genetic mapping in wheat indicated that all inversions had breakpoints in the low-recombinant gene-poor pericentromeric regions.These differences could have significant implications in wheat genome research where comparative approaches are used such as in ordering and orientating sequence contigs and in gene cloning.
Affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.Show MeSH
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Mentions: Twenty of the genes indicative of putative intrachromosomal rearrangements were further assessed against the euploid, isomic-tetrasomic, and ditelosomic lines of CS (supplementary table S1, Supplementary Material online). Primers designed for four of these genes (Contig66257, TRIUR3_10150, TRIUR3_35286, Bradi1g52600.1) failed to amplify. PCR products were successfully obtained for the other 16 genes, and chromosome arm locations deduced from the chromosome shotgun sequences from the IWGSC were confirmed for all of them (fig. 1).Fig. 1.—
Affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.