Limits...
Extensive pericentric rearrangements in the bread wheat (Triticum aestivum L.) genotype "Chinese Spring" revealed from chromosome shotgun sequence data.

Ma J, Stiller J, Wei Y, Zheng YL, Devos KM, Doležel J, Liu C - Genome Biol Evol (2014)

Bottom Line: Based on patterns of their homoeologous arm locations, 551 genes indicated the presence of pericentric inversions in at least 10 of the 21 chromosomes.Available data from deletion bin-mapped expressed sequence tags and genetic mapping in wheat indicated that all inversions had breakpoints in the low-recombinant gene-poor pericentromeric regions.These differences could have significant implications in wheat genome research where comparative approaches are used such as in ordering and orientating sequence contigs and in gene cloning.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.

Show MeSH

Related in: MedlinePlus

PCR profiles of the hexaploid wheat genotype CS i-tetrasomic (NT) and ditelosomic (DT) lines with primers for three of the genes involved in putative intrachromosomal rearrangements. Locations of the genes detected from the chromosome shotgun sequences are given in brackets following each gene. One kilobase pair (kb) plus DNA ladder was used as size marker (M).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4255769&req=5

evu237-F1: PCR profiles of the hexaploid wheat genotype CS i-tetrasomic (NT) and ditelosomic (DT) lines with primers for three of the genes involved in putative intrachromosomal rearrangements. Locations of the genes detected from the chromosome shotgun sequences are given in brackets following each gene. One kilobase pair (kb) plus DNA ladder was used as size marker (M).

Mentions: Twenty of the genes indicative of putative intrachromosomal rearrangements were further assessed against the euploid, isomic-tetrasomic, and ditelosomic lines of CS (supplementary table S1, Supplementary Material online). Primers designed for four of these genes (Contig66257, TRIUR3_10150, TRIUR3_35286, Bradi1g52600.1) failed to amplify. PCR products were successfully obtained for the other 16 genes, and chromosome arm locations deduced from the chromosome shotgun sequences from the IWGSC were confirmed for all of them (fig. 1).Fig. 1.—


Extensive pericentric rearrangements in the bread wheat (Triticum aestivum L.) genotype "Chinese Spring" revealed from chromosome shotgun sequence data.

Ma J, Stiller J, Wei Y, Zheng YL, Devos KM, Doležel J, Liu C - Genome Biol Evol (2014)

PCR profiles of the hexaploid wheat genotype CS i-tetrasomic (NT) and ditelosomic (DT) lines with primers for three of the genes involved in putative intrachromosomal rearrangements. Locations of the genes detected from the chromosome shotgun sequences are given in brackets following each gene. One kilobase pair (kb) plus DNA ladder was used as size marker (M).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255769&req=5

evu237-F1: PCR profiles of the hexaploid wheat genotype CS i-tetrasomic (NT) and ditelosomic (DT) lines with primers for three of the genes involved in putative intrachromosomal rearrangements. Locations of the genes detected from the chromosome shotgun sequences are given in brackets following each gene. One kilobase pair (kb) plus DNA ladder was used as size marker (M).
Mentions: Twenty of the genes indicative of putative intrachromosomal rearrangements were further assessed against the euploid, isomic-tetrasomic, and ditelosomic lines of CS (supplementary table S1, Supplementary Material online). Primers designed for four of these genes (Contig66257, TRIUR3_10150, TRIUR3_35286, Bradi1g52600.1) failed to amplify. PCR products were successfully obtained for the other 16 genes, and chromosome arm locations deduced from the chromosome shotgun sequences from the IWGSC were confirmed for all of them (fig. 1).Fig. 1.—

Bottom Line: Based on patterns of their homoeologous arm locations, 551 genes indicated the presence of pericentric inversions in at least 10 of the 21 chromosomes.Available data from deletion bin-mapped expressed sequence tags and genetic mapping in wheat indicated that all inversions had breakpoints in the low-recombinant gene-poor pericentromeric regions.These differences could have significant implications in wheat genome research where comparative approaches are used such as in ordering and orientating sequence contigs and in gene cloning.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.

Show MeSH
Related in: MedlinePlus