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Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae).

Zepeda-Cisneros CS, Meza Hernández JS, García-Martínez V, Ibañez-Palacios J, Zacharopoulou A, Franz G - BMC Genet. (2014)

Bottom Line: To increase the efficiency of this technique, we have developed a genetic sexing strain (GSS) in which the sexing mechanism is based on a pupal colour dimorphism (brown-black) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the black pupae (bp) locus.The translocation strain named Tapachula-7 showed minimal effect on survival and the best genetic stability of all ten strains.The present work is the first report of the construction of GSS of Anastrepha ludens, with potential use in a future Moscafrut operational program.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Anastrepha ludens is among the pests that have a major impact on México's economy because it attacks fruits as citrus and mangoes. The Mexican Federal government uses integrated pest management to control A. ludens through the Programa Nacional Moscas de la Fruta [National Fruit Fly Program, SAGARPA-SENASICA]. One of the main components of this program is the sterile insect technique (SIT), which is used to control field populations of the pest by releasing sterile flies.

Results: To increase the efficiency of this technique, we have developed a genetic sexing strain (GSS) in which the sexing mechanism is based on a pupal colour dimorphism (brown-black) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the black pupae (bp) locus. Ten strains producing wild-type (brown pupae) males and mutant (black pupae) females were isolated. Subsequent evaluations for several generations were performed in most of these strains. The translocation strain named Tapachula-7 showed minimal effect on survival and the best genetic stability of all ten strains. Genetic and cytogenetic analyses were performed using mitotic and polytene chromosomes and we succeeded to characterize the chromosomal structure of this reciprocal translocation and map the autosome breakpoint, despite the fact that the Y chromosome is not visible in polytene nuclei following standard staining.

Conclusions: We show that mitotic and polytene chromosomes can be used in cytogenetic analyses towards the development of genetic control methods in this pest species. The present work is the first report of the construction of GSS of Anastrepha ludens, with potential use in a future Moscafrut operational program.

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Crossing scheme for the isolation of genetic sexing strains in A. ludens. X and Y: represent the sex chromosomes. bp+: represents the autosome harboring the wild type allele for the brown pupae color. bp: represents the autosome harboring the black pupae (bp) mutation. Y-2 and 2-Y: represent the two new chromosomes following the translocation event.
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Figure 7: Crossing scheme for the isolation of genetic sexing strains in A. ludens. X and Y: represent the sex chromosomes. bp+: represents the autosome harboring the wild type allele for the brown pupae color. bp: represents the autosome harboring the black pupae (bp) mutation. Y-2 and 2-Y: represent the two new chromosomes following the translocation event.

Mentions: The F1 males of that cross were singly backcrossed to mutant females. If the F1 males carry a translocation between the Y and the autosome bearing the selectable marker "bp", all the males of a particular family will be wild-type, and all the females will be mutant (Figure 7). Where such a family was detected in the screen, the progeny was further propagated, and their true-breeding nature was ascertained and maintained in the laboratory for several generations. In each generation, the percentage of the hatched eggs, the number of pupae and their colour as well as the number of adults were recorded. When recombinants, i.e. females emerging from brown and males from black pupae, were detected they were counted and removed. The pupal colour separation was performed manually, and the correct classification was corroborated in adults using the dark coloration of the black pupae strain for differentiation.


Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae).

Zepeda-Cisneros CS, Meza Hernández JS, García-Martínez V, Ibañez-Palacios J, Zacharopoulou A, Franz G - BMC Genet. (2014)

Crossing scheme for the isolation of genetic sexing strains in A. ludens. X and Y: represent the sex chromosomes. bp+: represents the autosome harboring the wild type allele for the brown pupae color. bp: represents the autosome harboring the black pupae (bp) mutation. Y-2 and 2-Y: represent the two new chromosomes following the translocation event.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255765&req=5

Figure 7: Crossing scheme for the isolation of genetic sexing strains in A. ludens. X and Y: represent the sex chromosomes. bp+: represents the autosome harboring the wild type allele for the brown pupae color. bp: represents the autosome harboring the black pupae (bp) mutation. Y-2 and 2-Y: represent the two new chromosomes following the translocation event.
Mentions: The F1 males of that cross were singly backcrossed to mutant females. If the F1 males carry a translocation between the Y and the autosome bearing the selectable marker "bp", all the males of a particular family will be wild-type, and all the females will be mutant (Figure 7). Where such a family was detected in the screen, the progeny was further propagated, and their true-breeding nature was ascertained and maintained in the laboratory for several generations. In each generation, the percentage of the hatched eggs, the number of pupae and their colour as well as the number of adults were recorded. When recombinants, i.e. females emerging from brown and males from black pupae, were detected they were counted and removed. The pupal colour separation was performed manually, and the correct classification was corroborated in adults using the dark coloration of the black pupae strain for differentiation.

Bottom Line: To increase the efficiency of this technique, we have developed a genetic sexing strain (GSS) in which the sexing mechanism is based on a pupal colour dimorphism (brown-black) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the black pupae (bp) locus.The translocation strain named Tapachula-7 showed minimal effect on survival and the best genetic stability of all ten strains.The present work is the first report of the construction of GSS of Anastrepha ludens, with potential use in a future Moscafrut operational program.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Anastrepha ludens is among the pests that have a major impact on México's economy because it attacks fruits as citrus and mangoes. The Mexican Federal government uses integrated pest management to control A. ludens through the Programa Nacional Moscas de la Fruta [National Fruit Fly Program, SAGARPA-SENASICA]. One of the main components of this program is the sterile insect technique (SIT), which is used to control field populations of the pest by releasing sterile flies.

Results: To increase the efficiency of this technique, we have developed a genetic sexing strain (GSS) in which the sexing mechanism is based on a pupal colour dimorphism (brown-black) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the black pupae (bp) locus. Ten strains producing wild-type (brown pupae) males and mutant (black pupae) females were isolated. Subsequent evaluations for several generations were performed in most of these strains. The translocation strain named Tapachula-7 showed minimal effect on survival and the best genetic stability of all ten strains. Genetic and cytogenetic analyses were performed using mitotic and polytene chromosomes and we succeeded to characterize the chromosomal structure of this reciprocal translocation and map the autosome breakpoint, despite the fact that the Y chromosome is not visible in polytene nuclei following standard staining.

Conclusions: We show that mitotic and polytene chromosomes can be used in cytogenetic analyses towards the development of genetic control methods in this pest species. The present work is the first report of the construction of GSS of Anastrepha ludens, with potential use in a future Moscafrut operational program.

Show MeSH
Related in: MedlinePlus