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Parenteral nutrition-associated liver injury and increased GRP94 expression prevented by ω-3 fish oil-based lipid emulsion supplementation.

Zhu X, Xiao Z, Chen X, Li Y, Zhang X, Xu Y, Feng X, Wang J - J. Pediatr. Gastroenterol. Nutr. (2014)

Bottom Line: Reverse-transcriptase polymerase chain reaction was used to evaluate the messenger RNA levels of the endoplasmic reticulum stress chaperone protein glucose-regulated protein 94 (GRP94) in liver tissues and GRP94 protein levels were compared through immunohistochemistry and Western blot assays.TPN-soy animals had significantly higher serum total bilirubin, direct bilirubin, and γ-glutamyl transpeptidase and lower serum albumin than the controls (P < 0.01, each) or the TPN-FO group, which were similar to the controls (P < 0.01 cf.TPN).

View Article: PubMed Central - PubMed

Affiliation: *Department of Neonatology †Institute of Pediatric Research ‡Department of Neonatology Surgery, Children's Hospital Affiliated to Soochow University, Suzhou, Jiangsu, China.

ABSTRACT

Objective: Parenteral nutrition in infants with gastrointestinal disorders can be lifesaving, but it is also associated with parenteral nutrition-associated liver disease. We investigated the effects of incorporating ω-3 fish oil in a parenteral nutrition mixture on signs of parenteral nutrition-associated liver disease and explored the mechanism involved in this process.

Methods: Seven-day-old New Zealand rabbits were divided into 3 groups of 8, and for 1 week they were infused via the right jugular vein with standard total parenteral nutrition with soybean oil (TPN-soy) or TPN with ω-3 fish oil-based lipid emulsion (TPN-FO), or naturally nursed with rabbit milk (control). Serum and liver tissues were analyzed for serological indicators and pathology, respectively. Reverse-transcriptase polymerase chain reaction was used to evaluate the messenger RNA levels of the endoplasmic reticulum stress chaperone protein glucose-regulated protein 94 (GRP94) in liver tissues and GRP94 protein levels were compared through immunohistochemistry and Western blot assays.

Results: TPN-soy animals had significantly higher serum total bilirubin, direct bilirubin, and γ-glutamyl transpeptidase and lower serum albumin than the controls (P < 0.01, each) or the TPN-FO group, which were similar to the controls (P < 0.01 cf. TPN). Damage to liver tissues of the TPN-FO group was much less than that of the TPN-soy group. GRP94 messenger RNA and protein levels in liver tissues of TPN-soy animals were significantly higher than that of the controls or TPN-FO rabbits, which were similar to the controls.

Conclusions: Incorporating ω-3 fish oil in parenteral nutrition emulsion greatly prevented liver dysfunction and liver tissue damage in week-old rabbit kits, possibly by preventing endoplasmic reticulum stress.

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Related in: MedlinePlus

Immunohistological analysis of GRP94 protein in liver tissues. Representative results of GRP94 staining in samples derived from (A) control, (B) TPN-soy group, and (C) TPN-FO group. Quantitative data (D). Original magnification ×400. #P < 0.01, compared with control; ∗P < 0.01, compared with TPN-soy. GRP94 = glucose-regulated protein 94; TPN = total parenteral nutrition; TPN-FO = TPN with ω-3 fish oil; TPN-soy = TPN with soybean oil.
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Figure 3: Immunohistological analysis of GRP94 protein in liver tissues. Representative results of GRP94 staining in samples derived from (A) control, (B) TPN-soy group, and (C) TPN-FO group. Quantitative data (D). Original magnification ×400. #P < 0.01, compared with control; ∗P < 0.01, compared with TPN-soy. GRP94 = glucose-regulated protein 94; TPN = total parenteral nutrition; TPN-FO = TPN with ω-3 fish oil; TPN-soy = TPN with soybean oil.

Mentions: Immunostaining of these tissues showed that GRP94 protein levels in the liver tissues of the TPN-soy group (133.84 ± 13.66, referenced to the gray value standard) were significantly higher than those of the controls (78.14 ± 8.17, P < 0.01; Fig. 3) and also significantly higher than the GRP94 protein levels of the TPN-FO group (80.73 ± 9.36, P < 0.01), whereas the GRP94 protein levels of the TPN-FO and controls were similar.


Parenteral nutrition-associated liver injury and increased GRP94 expression prevented by ω-3 fish oil-based lipid emulsion supplementation.

Zhu X, Xiao Z, Chen X, Li Y, Zhang X, Xu Y, Feng X, Wang J - J. Pediatr. Gastroenterol. Nutr. (2014)

Immunohistological analysis of GRP94 protein in liver tissues. Representative results of GRP94 staining in samples derived from (A) control, (B) TPN-soy group, and (C) TPN-FO group. Quantitative data (D). Original magnification ×400. #P < 0.01, compared with control; ∗P < 0.01, compared with TPN-soy. GRP94 = glucose-regulated protein 94; TPN = total parenteral nutrition; TPN-FO = TPN with ω-3 fish oil; TPN-soy = TPN with soybean oil.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4255760&req=5

Figure 3: Immunohistological analysis of GRP94 protein in liver tissues. Representative results of GRP94 staining in samples derived from (A) control, (B) TPN-soy group, and (C) TPN-FO group. Quantitative data (D). Original magnification ×400. #P < 0.01, compared with control; ∗P < 0.01, compared with TPN-soy. GRP94 = glucose-regulated protein 94; TPN = total parenteral nutrition; TPN-FO = TPN with ω-3 fish oil; TPN-soy = TPN with soybean oil.
Mentions: Immunostaining of these tissues showed that GRP94 protein levels in the liver tissues of the TPN-soy group (133.84 ± 13.66, referenced to the gray value standard) were significantly higher than those of the controls (78.14 ± 8.17, P < 0.01; Fig. 3) and also significantly higher than the GRP94 protein levels of the TPN-FO group (80.73 ± 9.36, P < 0.01), whereas the GRP94 protein levels of the TPN-FO and controls were similar.

Bottom Line: Reverse-transcriptase polymerase chain reaction was used to evaluate the messenger RNA levels of the endoplasmic reticulum stress chaperone protein glucose-regulated protein 94 (GRP94) in liver tissues and GRP94 protein levels were compared through immunohistochemistry and Western blot assays.TPN-soy animals had significantly higher serum total bilirubin, direct bilirubin, and γ-glutamyl transpeptidase and lower serum albumin than the controls (P < 0.01, each) or the TPN-FO group, which were similar to the controls (P < 0.01 cf.TPN).

View Article: PubMed Central - PubMed

Affiliation: *Department of Neonatology †Institute of Pediatric Research ‡Department of Neonatology Surgery, Children's Hospital Affiliated to Soochow University, Suzhou, Jiangsu, China.

ABSTRACT

Objective: Parenteral nutrition in infants with gastrointestinal disorders can be lifesaving, but it is also associated with parenteral nutrition-associated liver disease. We investigated the effects of incorporating ω-3 fish oil in a parenteral nutrition mixture on signs of parenteral nutrition-associated liver disease and explored the mechanism involved in this process.

Methods: Seven-day-old New Zealand rabbits were divided into 3 groups of 8, and for 1 week they were infused via the right jugular vein with standard total parenteral nutrition with soybean oil (TPN-soy) or TPN with ω-3 fish oil-based lipid emulsion (TPN-FO), or naturally nursed with rabbit milk (control). Serum and liver tissues were analyzed for serological indicators and pathology, respectively. Reverse-transcriptase polymerase chain reaction was used to evaluate the messenger RNA levels of the endoplasmic reticulum stress chaperone protein glucose-regulated protein 94 (GRP94) in liver tissues and GRP94 protein levels were compared through immunohistochemistry and Western blot assays.

Results: TPN-soy animals had significantly higher serum total bilirubin, direct bilirubin, and γ-glutamyl transpeptidase and lower serum albumin than the controls (P < 0.01, each) or the TPN-FO group, which were similar to the controls (P < 0.01 cf. TPN). Damage to liver tissues of the TPN-FO group was much less than that of the TPN-soy group. GRP94 messenger RNA and protein levels in liver tissues of TPN-soy animals were significantly higher than that of the controls or TPN-FO rabbits, which were similar to the controls.

Conclusions: Incorporating ω-3 fish oil in parenteral nutrition emulsion greatly prevented liver dysfunction and liver tissue damage in week-old rabbit kits, possibly by preventing endoplasmic reticulum stress.

Show MeSH
Related in: MedlinePlus