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Alternative treatment of serious and mild Pasteurella multocida infection in New Zealand White rabbits.

Palócz O, Gál J, Clayton P, Dinya Z, Somogyi Z, Juhász C, Csikó G - BMC Vet. Res. (2014)

Bottom Line: Immunisation is only variably effective.Four other groups were inoculated both in. and intramuscularly (im.), representing a supra-physiological challenge, and received either (1-3), (1-6) β-glucans, antibiotic or placebo. β-glucans given prophylactically were highly effective in protecting against physiological (in.) bacterial challenge.They were less effective in protecting against supra-physiological bacterial challenge (in. and im.), although they extended survival times.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest, 1078, Hungary. palocz.orsolya@aotk.szie.hu.

ABSTRACT

Background: Pasteurella multocida causes numerous economically relevant diseases in livestock including rabbits. Immunisation is only variably effective. Prophylactic antibiotics are used in some species but are contra-indicated in rabbits, due to their adverse effects on the rabbit microbiota. There is therefore a substantial need for alternative forms of infection control in rabbits; we investigated the effect of oral β-glucan on P. multocida infection in this species.

Results: Thirthy-five New Zealand White rabbits were randomly divided into five groups of seven animals. Three groups were inoculated with Pasteurella multocida intranasally (in.), a physiologically appropriate challenge which reproduces naturally acquired infection, and received either (1-3), (1-6) β-glucans or placebo. Four other groups were inoculated both in. and intramuscularly (im.), representing a supra-physiological challenge, and received either (1-3), (1-6) β-glucans, antibiotic or placebo. β-glucans given prophylactically were highly effective in protecting against physiological (in.) bacterial challenge. They were less effective in protecting against supra-physiological bacterial challenge (in. and im.), although they extended survival times. This latter finding has practical relevance to breeders as it extends the window in which heavily infected and symptomatic animals can be salvaged with antibiotics.

Conclusions: In our study, (1-3), (1-6) β-glucans were highly effective in protecting against a model of naturally acquired P. multocida infection and extended survival times in the supra-physiological model. Enrofloxacin was effective in protecting against supra-physiological infection. We are currently reviewing the use of combined prophylaxis.

No MeSH data available.


Related in: MedlinePlus

Impact of β-glucan treatements on rabbits infected intranasally and intramuscularly withP. multocida. Rabbits were received methylcellulose solution (positive controls, n = 7) and either low-dose (n = 7) or high-dose (n = 7) β-glucan (5 or 50 mg/kg bw.). Curves were calculated by Kaplan-Meier method and Cox’s F test was used for comparisons between treatments. Positive control x low-dose β-glucan (F = 2.306, p = 0.065), positive control x high-dose β-glucan (F = 2.344, p = 0.065), positive control x both β-glucan treatments (F = 2.408, p = 0.044).
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Fig4: Impact of β-glucan treatements on rabbits infected intranasally and intramuscularly withP. multocida. Rabbits were received methylcellulose solution (positive controls, n = 7) and either low-dose (n = 7) or high-dose (n = 7) β-glucan (5 or 50 mg/kg bw.). Curves were calculated by Kaplan-Meier method and Cox’s F test was used for comparisons between treatments. Positive control x low-dose β-glucan (F = 2.306, p = 0.065), positive control x high-dose β-glucan (F = 2.344, p = 0.065), positive control x both β-glucan treatments (F = 2.408, p = 0.044).

Mentions: β-glucan treatment did not reduce the numbers of deaths in the supra-physiological model but did postpone death by several days. Either low-dose or high-dose β-glucan treatment marginally delayed death but the delay was statistically significant when both β-glucan treatments were compared to the positive control group together (Figure 4). In field conditions, this extended window of opportunity for antibiotic intervention would have considerable implications for veterinary management.Figure 4


Alternative treatment of serious and mild Pasteurella multocida infection in New Zealand White rabbits.

Palócz O, Gál J, Clayton P, Dinya Z, Somogyi Z, Juhász C, Csikó G - BMC Vet. Res. (2014)

Impact of β-glucan treatements on rabbits infected intranasally and intramuscularly withP. multocida. Rabbits were received methylcellulose solution (positive controls, n = 7) and either low-dose (n = 7) or high-dose (n = 7) β-glucan (5 or 50 mg/kg bw.). Curves were calculated by Kaplan-Meier method and Cox’s F test was used for comparisons between treatments. Positive control x low-dose β-glucan (F = 2.306, p = 0.065), positive control x high-dose β-glucan (F = 2.344, p = 0.065), positive control x both β-glucan treatments (F = 2.408, p = 0.044).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4248437&req=5

Fig4: Impact of β-glucan treatements on rabbits infected intranasally and intramuscularly withP. multocida. Rabbits were received methylcellulose solution (positive controls, n = 7) and either low-dose (n = 7) or high-dose (n = 7) β-glucan (5 or 50 mg/kg bw.). Curves were calculated by Kaplan-Meier method and Cox’s F test was used for comparisons between treatments. Positive control x low-dose β-glucan (F = 2.306, p = 0.065), positive control x high-dose β-glucan (F = 2.344, p = 0.065), positive control x both β-glucan treatments (F = 2.408, p = 0.044).
Mentions: β-glucan treatment did not reduce the numbers of deaths in the supra-physiological model but did postpone death by several days. Either low-dose or high-dose β-glucan treatment marginally delayed death but the delay was statistically significant when both β-glucan treatments were compared to the positive control group together (Figure 4). In field conditions, this extended window of opportunity for antibiotic intervention would have considerable implications for veterinary management.Figure 4

Bottom Line: Immunisation is only variably effective.Four other groups were inoculated both in. and intramuscularly (im.), representing a supra-physiological challenge, and received either (1-3), (1-6) β-glucans, antibiotic or placebo. β-glucans given prophylactically were highly effective in protecting against physiological (in.) bacterial challenge.They were less effective in protecting against supra-physiological bacterial challenge (in. and im.), although they extended survival times.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest, 1078, Hungary. palocz.orsolya@aotk.szie.hu.

ABSTRACT

Background: Pasteurella multocida causes numerous economically relevant diseases in livestock including rabbits. Immunisation is only variably effective. Prophylactic antibiotics are used in some species but are contra-indicated in rabbits, due to their adverse effects on the rabbit microbiota. There is therefore a substantial need for alternative forms of infection control in rabbits; we investigated the effect of oral β-glucan on P. multocida infection in this species.

Results: Thirthy-five New Zealand White rabbits were randomly divided into five groups of seven animals. Three groups were inoculated with Pasteurella multocida intranasally (in.), a physiologically appropriate challenge which reproduces naturally acquired infection, and received either (1-3), (1-6) β-glucans or placebo. Four other groups were inoculated both in. and intramuscularly (im.), representing a supra-physiological challenge, and received either (1-3), (1-6) β-glucans, antibiotic or placebo. β-glucans given prophylactically were highly effective in protecting against physiological (in.) bacterial challenge. They were less effective in protecting against supra-physiological bacterial challenge (in. and im.), although they extended survival times. This latter finding has practical relevance to breeders as it extends the window in which heavily infected and symptomatic animals can be salvaged with antibiotics.

Conclusions: In our study, (1-3), (1-6) β-glucans were highly effective in protecting against a model of naturally acquired P. multocida infection and extended survival times in the supra-physiological model. Enrofloxacin was effective in protecting against supra-physiological infection. We are currently reviewing the use of combined prophylaxis.

No MeSH data available.


Related in: MedlinePlus