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Phytochemical analysis, antioxidant activity, fatty acids composition, and functional group analysis of Heliotropium bacciferum.

Ahmad S, Ahmad S, Bibi A, Ishaq MS, Afridi MS, Kanwal F, Zakir M, Fatima F - ScientificWorldJournal (2014)

Bottom Line: Ascorbic acid was used as a standard.The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage.The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan.

ABSTRACT
Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000 mg/mL) and then several dilutions (50, 100, 150, 200, and 250 mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard. The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated to mass spectrometer was used. The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.

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GC-MS chromatogram of the plant n-hexane extract with labeled signals detected by GC-MS detector (Analytes).
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fig5: GC-MS chromatogram of the plant n-hexane extract with labeled signals detected by GC-MS detector (Analytes).

Mentions: Table 6 viewing the names of fatty acids, area of relevant peaks, relative percentage compositions, times of the analysis, and retention time (R. time) was obtained from gas chromatography mass spectrometry (GC-MS) analysis. The percentage concentration and areas are the mean of the 3 measurements shown in Table 6. Figure 5 shows the obtained GC-MS chromatogram of the n-hexane extract of the plant Heliotropium bacciferum with regularly labeled signals detected by GC-MS detector (Analytes). In the sample under investigation, the saturated and the unsaturated fatty acids were found (Figure 4).


Phytochemical analysis, antioxidant activity, fatty acids composition, and functional group analysis of Heliotropium bacciferum.

Ahmad S, Ahmad S, Bibi A, Ishaq MS, Afridi MS, Kanwal F, Zakir M, Fatima F - ScientificWorldJournal (2014)

GC-MS chromatogram of the plant n-hexane extract with labeled signals detected by GC-MS detector (Analytes).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4247971&req=5

fig5: GC-MS chromatogram of the plant n-hexane extract with labeled signals detected by GC-MS detector (Analytes).
Mentions: Table 6 viewing the names of fatty acids, area of relevant peaks, relative percentage compositions, times of the analysis, and retention time (R. time) was obtained from gas chromatography mass spectrometry (GC-MS) analysis. The percentage concentration and areas are the mean of the 3 measurements shown in Table 6. Figure 5 shows the obtained GC-MS chromatogram of the n-hexane extract of the plant Heliotropium bacciferum with regularly labeled signals detected by GC-MS detector (Analytes). In the sample under investigation, the saturated and the unsaturated fatty acids were found (Figure 4).

Bottom Line: Ascorbic acid was used as a standard.The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage.The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan.

ABSTRACT
Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000 mg/mL) and then several dilutions (50, 100, 150, 200, and 250 mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard. The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated to mass spectrometer was used. The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.

Show MeSH