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Characterization of a selenium-tolerant rhizosphere strain from a novel Se-hyperaccumulating plant Cardamine hupingshanesis.

Tong X, Yuan L, Luo L, Yin X - ScientificWorldJournal (2014)

Bottom Line: A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory.Moreover, when exposed to 1.5 mg Se L(-1) or 15.0 mg Se L(-1), As absorption diminished in the logarithmic phase.In contrast, As absorption increased when exposed to 7.5 mg Se L(-1), indicating As metabolism processes could be affected by Se on this strain.

View Article: PubMed Central - PubMed

Affiliation: Advanced Lab for Selenium and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, China ; Nano Science and Technology School, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, China.

ABSTRACT
A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacteria play some roles during SeCys2 accumulation. Here, one Se-tolerant rhizosphere strain, Microbacterium oxydans, was isolated from C. hupingshanesis. Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. Furthermore, Se tolerance test indicated the strain could grow in extreme high Se level of 15.0 mg Se L(-1). When exposed to 1.5 mg Se L(-1), SeCys2 was the predominant Se species in the bacteria, consistent with the Se species in C. hupingshanesis. This coincidence might reveal that this strain played some positive effect in SeCys2 accumulation of C. hupingshanesis. Moreover, when exposed to 1.5 mg Se L(-1) or 15.0 mg Se L(-1), As absorption diminished in the logarithmic phase. In contrast, As absorption increased when exposed to 7.5 mg Se L(-1), indicating As metabolism processes could be affected by Se on this strain. The present study provided a sight on the role of rhizosphere bacteria during Se accumulation for Se-hyperaccumulating plant.

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Related in: MedlinePlus

The growth of the isolated strain, Suzhou 08+6, in the culture medium with different concentration of Se and As. One control group and four experimental groups with 0.5 mg As L−1 and different concentration of Se (0, 1.5 mg Se L−1, 7.5 mg Se L−1, and 15.0 mg Se L−1) in the culture medium (n = 5).
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fig6: The growth of the isolated strain, Suzhou 08+6, in the culture medium with different concentration of Se and As. One control group and four experimental groups with 0.5 mg As L−1 and different concentration of Se (0, 1.5 mg Se L−1, 7.5 mg Se L−1, and 15.0 mg Se L−1) in the culture medium (n = 5).

Mentions: To study the interactions between Se and As, Suzhou 08+6 was grown in liquid TSB with different concentrations of selenite and arsenic (Table 1). The results displayed that Suzhou 08+6 could grow in five different culture mediums (Figure 6). Overall, there were no obvious differences among CK, As 0.5 + Se 1.5, and As 0.5 + Se 7.5. However, high concentration of selenite (15.0 mg Se L−1) might have a strong inhibited effect on strain growth during the culture period in As 0.5 + Se 15.0 group.


Characterization of a selenium-tolerant rhizosphere strain from a novel Se-hyperaccumulating plant Cardamine hupingshanesis.

Tong X, Yuan L, Luo L, Yin X - ScientificWorldJournal (2014)

The growth of the isolated strain, Suzhou 08+6, in the culture medium with different concentration of Se and As. One control group and four experimental groups with 0.5 mg As L−1 and different concentration of Se (0, 1.5 mg Se L−1, 7.5 mg Se L−1, and 15.0 mg Se L−1) in the culture medium (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4247970&req=5

fig6: The growth of the isolated strain, Suzhou 08+6, in the culture medium with different concentration of Se and As. One control group and four experimental groups with 0.5 mg As L−1 and different concentration of Se (0, 1.5 mg Se L−1, 7.5 mg Se L−1, and 15.0 mg Se L−1) in the culture medium (n = 5).
Mentions: To study the interactions between Se and As, Suzhou 08+6 was grown in liquid TSB with different concentrations of selenite and arsenic (Table 1). The results displayed that Suzhou 08+6 could grow in five different culture mediums (Figure 6). Overall, there were no obvious differences among CK, As 0.5 + Se 1.5, and As 0.5 + Se 7.5. However, high concentration of selenite (15.0 mg Se L−1) might have a strong inhibited effect on strain growth during the culture period in As 0.5 + Se 15.0 group.

Bottom Line: A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory.Moreover, when exposed to 1.5 mg Se L(-1) or 15.0 mg Se L(-1), As absorption diminished in the logarithmic phase.In contrast, As absorption increased when exposed to 7.5 mg Se L(-1), indicating As metabolism processes could be affected by Se on this strain.

View Article: PubMed Central - PubMed

Affiliation: Advanced Lab for Selenium and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, China ; Nano Science and Technology School, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, China.

ABSTRACT
A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacteria play some roles during SeCys2 accumulation. Here, one Se-tolerant rhizosphere strain, Microbacterium oxydans, was isolated from C. hupingshanesis. Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. Furthermore, Se tolerance test indicated the strain could grow in extreme high Se level of 15.0 mg Se L(-1). When exposed to 1.5 mg Se L(-1), SeCys2 was the predominant Se species in the bacteria, consistent with the Se species in C. hupingshanesis. This coincidence might reveal that this strain played some positive effect in SeCys2 accumulation of C. hupingshanesis. Moreover, when exposed to 1.5 mg Se L(-1) or 15.0 mg Se L(-1), As absorption diminished in the logarithmic phase. In contrast, As absorption increased when exposed to 7.5 mg Se L(-1), indicating As metabolism processes could be affected by Se on this strain. The present study provided a sight on the role of rhizosphere bacteria during Se accumulation for Se-hyperaccumulating plant.

Show MeSH
Related in: MedlinePlus