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Comparative hair restorer efficacy of medicinal herb on nude (Foxn1nu) mice.

Begum S, Lee MR, Gu LJ, Hossain MJ, Kim HK, Sung CK - Biomed Res Int (2014)

Bottom Line: The presence of follicular keratinocytes was confirmed by BrdU labeling, S-phase cells in HFs.Therefore, Eclipta alba extract and/or phytochemicals strongly displayed incomparability of hair growth promotion activity than others.Thus, the standardized Eclipta alba extract can be used as an effective, alternative, and complementary treatment against hair loss.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.

ABSTRACT
Eclipta alba (L.) Hassk, Asiasarum sieboldii (Miq.) F. Maek (Asiasari radix), and Panax ginseng C. A. Mey (red ginseng) are traditionally acclaimed for therapeutic properties of various human ailments. Synergistic effect of each standardized plant extract was investigated for hair growth potential on nude mice, as these mutant mice genetically lack hair due to abnormal keratinization. Dried plant samples were ground and extracted by methanol. Topical application was performed on the back of nude mice daily up to completion of two hair growth generations. The hair density and length of Eclipta alba treated mice were increased significantly (P>0.001) than control mice. Hair growth area was also distinctly visible in Eclipta alba treated mice. On the other hand, Asiasari radix and Panax ginseng treated mice developing hair loss were recognized from the abortive boundaries of hair coverage. Histomorphometric observation of nude mice skin samples revealed an increase in number of hair follicles (HFs). The presence of follicular keratinocytes was confirmed by BrdU labeling, S-phase cells in HFs. Therefore, Eclipta alba extract and/or phytochemicals strongly displayed incomparability of hair growth promotion activity than others. Thus, the standardized Eclipta alba extract can be used as an effective, alternative, and complementary treatment against hair loss.

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Effect of different plant extract on hair coverage area in athymic nude mice. Mice were evaluated on a scale of “0–4” parameters as follows: “0” = complete alopecia; “1” = <25% hair coverage; “2” = 25–50% hair coverage; “3” = 50–75% hair coverage; “4” = full hair coverage. The different letters (a and b) within a column indicate significant differences (P < 0.05) determined by Duncan's multiple range test.
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fig2: Effect of different plant extract on hair coverage area in athymic nude mice. Mice were evaluated on a scale of “0–4” parameters as follows: “0” = complete alopecia; “1” = <25% hair coverage; “2” = 25–50% hair coverage; “3” = 50–75% hair coverage; “4” = full hair coverage. The different letters (a and b) within a column indicate significant differences (P < 0.05) determined by Duncan's multiple range test.

Mentions: The effects on hair coverage area of all the treated groups were precisely estimated for each mouse in both first and second hair growth cycle by giving them a score from “0” to “4.” The maximum hair growth score was significantly (P < 0.001) increased in mice treated with minoxidil and E. alba than control group during first and second hair growth cycles (Figure 2). On the other hand, A. radix treated group also consistently effective for inducing hair coverage area (2.25 ± 0.82) in first hair cycle but transient hair coat and progressive hair loss turn to similar score (1.25 ± 0.5) with that of control group (1.25 ± 0.5) in second hair cycle. There was no significant difference in terms of the hair coverage area among P. ginseng and control group.


Comparative hair restorer efficacy of medicinal herb on nude (Foxn1nu) mice.

Begum S, Lee MR, Gu LJ, Hossain MJ, Kim HK, Sung CK - Biomed Res Int (2014)

Effect of different plant extract on hair coverage area in athymic nude mice. Mice were evaluated on a scale of “0–4” parameters as follows: “0” = complete alopecia; “1” = <25% hair coverage; “2” = 25–50% hair coverage; “3” = 50–75% hair coverage; “4” = full hair coverage. The different letters (a and b) within a column indicate significant differences (P < 0.05) determined by Duncan's multiple range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4247959&req=5

fig2: Effect of different plant extract on hair coverage area in athymic nude mice. Mice were evaluated on a scale of “0–4” parameters as follows: “0” = complete alopecia; “1” = <25% hair coverage; “2” = 25–50% hair coverage; “3” = 50–75% hair coverage; “4” = full hair coverage. The different letters (a and b) within a column indicate significant differences (P < 0.05) determined by Duncan's multiple range test.
Mentions: The effects on hair coverage area of all the treated groups were precisely estimated for each mouse in both first and second hair growth cycle by giving them a score from “0” to “4.” The maximum hair growth score was significantly (P < 0.001) increased in mice treated with minoxidil and E. alba than control group during first and second hair growth cycles (Figure 2). On the other hand, A. radix treated group also consistently effective for inducing hair coverage area (2.25 ± 0.82) in first hair cycle but transient hair coat and progressive hair loss turn to similar score (1.25 ± 0.5) with that of control group (1.25 ± 0.5) in second hair cycle. There was no significant difference in terms of the hair coverage area among P. ginseng and control group.

Bottom Line: The presence of follicular keratinocytes was confirmed by BrdU labeling, S-phase cells in HFs.Therefore, Eclipta alba extract and/or phytochemicals strongly displayed incomparability of hair growth promotion activity than others.Thus, the standardized Eclipta alba extract can be used as an effective, alternative, and complementary treatment against hair loss.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.

ABSTRACT
Eclipta alba (L.) Hassk, Asiasarum sieboldii (Miq.) F. Maek (Asiasari radix), and Panax ginseng C. A. Mey (red ginseng) are traditionally acclaimed for therapeutic properties of various human ailments. Synergistic effect of each standardized plant extract was investigated for hair growth potential on nude mice, as these mutant mice genetically lack hair due to abnormal keratinization. Dried plant samples were ground and extracted by methanol. Topical application was performed on the back of nude mice daily up to completion of two hair growth generations. The hair density and length of Eclipta alba treated mice were increased significantly (P>0.001) than control mice. Hair growth area was also distinctly visible in Eclipta alba treated mice. On the other hand, Asiasari radix and Panax ginseng treated mice developing hair loss were recognized from the abortive boundaries of hair coverage. Histomorphometric observation of nude mice skin samples revealed an increase in number of hair follicles (HFs). The presence of follicular keratinocytes was confirmed by BrdU labeling, S-phase cells in HFs. Therefore, Eclipta alba extract and/or phytochemicals strongly displayed incomparability of hair growth promotion activity than others. Thus, the standardized Eclipta alba extract can be used as an effective, alternative, and complementary treatment against hair loss.

Show MeSH
Related in: MedlinePlus