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In vivo and in vitro antitumor effects of platycodin d, a saponin purified from platycodi radix on the h520 lung cancer cell.

Park JC, Lee YJ, Choi HY, Shin YK, Kim JD, Ku SK - Evid Based Complement Alternat Med (2014)

Bottom Line: The results were compared with gemcitabine 160 mg/kg intraperitoneally treated mice (7-day intervals).Platycodin D showed favorable cytotoxic effects on the H520 cells, and also dose-dependently decreased the tumor volumes and weights with increases of apoptotic cells (caspase-3 and PARP immunopositive cells), iNOS and TNF-α immunoreactivities, decreases of COX-2 immunoreactivities in tumor masses.Platycodin D also showed dose-dependent immunostimulatory and anticachexia effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Internal Medicine of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, No. 1 Haanydae-ro, Gyeongsan, Gyeongbuk 712-715, Republic of Korea.

ABSTRACT
Platycodin D is a major pharmacological constituent of Platycodi radix and has showed various pharmacological activities through oxidative stress defense mechanisms. Here, possible antitumor, anticachexia, and immunomodulatory activities of platycodin D were observed on the H520 tumor cell-bearing athymic nude mice after confirming the in vitro cytotoxicity. Platycodin D was orally administered at dose levels of 200, 100, and 50 mg/kg, once a day for 35 days from 15 days after implantation. The results were compared with gemcitabine 160 mg/kg intraperitoneally treated mice (7-day intervals). Platycodin D showed favorable cytotoxic effects on the H520 cells, and also dose-dependently decreased the tumor volumes and weights with increases of apoptotic cells (caspase-3 and PARP immunopositive cells), iNOS and TNF-α immunoreactivities, decreases of COX-2 immunoreactivities in tumor masses. Platycodin D also showed dose-dependent immunostimulatory and anticachexia effects. Gemcitabine showed favorable cytotoxity against H520 tumor cell and related in vivo antitumor effects but aggravated the cancer related cachexia and immunosuppress in H520 tumor cell-bearing athymic nude mice. Taken together, it is considered that oral treatment of platycodin D has potent antitumor activities on H520 cells through direct cytotoxic effects, increases of apoptosis in tumor cells, and immunostimulatory effects and can be control cancer related cachexia.

No MeSH data available.


Related in: MedlinePlus

Changes on the splenic and peritoneal NK cell activities in H520 tumor cell xenograft mice. Values are expressed Mean ± SD of eight mice (% specific 51Cr releases); TB = tumor-bearing; NK = natural killer; Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals; Platycodin D was orally administered, once a day; aP < 0.01 as compared with intact control by LSD test; bP < 0.01 as compared with TB control by LSD test; cP < 0.01 and dP < 0.05 as compared with intact control by MW test; eP < 0.01 and fP < 0.05 as compared with TB control by MW test.
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fig7: Changes on the splenic and peritoneal NK cell activities in H520 tumor cell xenograft mice. Values are expressed Mean ± SD of eight mice (% specific 51Cr releases); TB = tumor-bearing; NK = natural killer; Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals; Platycodin D was orally administered, once a day; aP < 0.01 as compared with intact control by LSD test; bP < 0.01 as compared with TB control by LSD test; cP < 0.01 and dP < 0.05 as compared with intact control by MW test; eP < 0.01 and fP < 0.05 as compared with TB control by MW test.

Mentions: Significant (P < 0.01) decreases of the splenic and peritoneal NK cell activities were observed in tumor-bearing control mice as compared with intact control mice, respectively. However, all three different dosages of platycodin D administered mice showed significant (P < 0.01) increases of the NK cell activities as compared with tumor-bearing control, dose-dependently. Gemcitabine administered mice showed marked decreases of the splenic NK cell activities and significant (P < 0.05) decreases of the peritoneal NK cell activities as compared with tumor-bearing control mice, respectively (Figure 7).


In vivo and in vitro antitumor effects of platycodin d, a saponin purified from platycodi radix on the h520 lung cancer cell.

Park JC, Lee YJ, Choi HY, Shin YK, Kim JD, Ku SK - Evid Based Complement Alternat Med (2014)

Changes on the splenic and peritoneal NK cell activities in H520 tumor cell xenograft mice. Values are expressed Mean ± SD of eight mice (% specific 51Cr releases); TB = tumor-bearing; NK = natural killer; Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals; Platycodin D was orally administered, once a day; aP < 0.01 as compared with intact control by LSD test; bP < 0.01 as compared with TB control by LSD test; cP < 0.01 and dP < 0.05 as compared with intact control by MW test; eP < 0.01 and fP < 0.05 as compared with TB control by MW test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4247928&req=5

fig7: Changes on the splenic and peritoneal NK cell activities in H520 tumor cell xenograft mice. Values are expressed Mean ± SD of eight mice (% specific 51Cr releases); TB = tumor-bearing; NK = natural killer; Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals; Platycodin D was orally administered, once a day; aP < 0.01 as compared with intact control by LSD test; bP < 0.01 as compared with TB control by LSD test; cP < 0.01 and dP < 0.05 as compared with intact control by MW test; eP < 0.01 and fP < 0.05 as compared with TB control by MW test.
Mentions: Significant (P < 0.01) decreases of the splenic and peritoneal NK cell activities were observed in tumor-bearing control mice as compared with intact control mice, respectively. However, all three different dosages of platycodin D administered mice showed significant (P < 0.01) increases of the NK cell activities as compared with tumor-bearing control, dose-dependently. Gemcitabine administered mice showed marked decreases of the splenic NK cell activities and significant (P < 0.05) decreases of the peritoneal NK cell activities as compared with tumor-bearing control mice, respectively (Figure 7).

Bottom Line: The results were compared with gemcitabine 160 mg/kg intraperitoneally treated mice (7-day intervals).Platycodin D showed favorable cytotoxic effects on the H520 cells, and also dose-dependently decreased the tumor volumes and weights with increases of apoptotic cells (caspase-3 and PARP immunopositive cells), iNOS and TNF-α immunoreactivities, decreases of COX-2 immunoreactivities in tumor masses.Platycodin D also showed dose-dependent immunostimulatory and anticachexia effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Internal Medicine of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, No. 1 Haanydae-ro, Gyeongsan, Gyeongbuk 712-715, Republic of Korea.

ABSTRACT
Platycodin D is a major pharmacological constituent of Platycodi radix and has showed various pharmacological activities through oxidative stress defense mechanisms. Here, possible antitumor, anticachexia, and immunomodulatory activities of platycodin D were observed on the H520 tumor cell-bearing athymic nude mice after confirming the in vitro cytotoxicity. Platycodin D was orally administered at dose levels of 200, 100, and 50 mg/kg, once a day for 35 days from 15 days after implantation. The results were compared with gemcitabine 160 mg/kg intraperitoneally treated mice (7-day intervals). Platycodin D showed favorable cytotoxic effects on the H520 cells, and also dose-dependently decreased the tumor volumes and weights with increases of apoptotic cells (caspase-3 and PARP immunopositive cells), iNOS and TNF-α immunoreactivities, decreases of COX-2 immunoreactivities in tumor masses. Platycodin D also showed dose-dependent immunostimulatory and anticachexia effects. Gemcitabine showed favorable cytotoxity against H520 tumor cell and related in vivo antitumor effects but aggravated the cancer related cachexia and immunosuppress in H520 tumor cell-bearing athymic nude mice. Taken together, it is considered that oral treatment of platycodin D has potent antitumor activities on H520 cells through direct cytotoxic effects, increases of apoptosis in tumor cells, and immunostimulatory effects and can be control cancer related cachexia.

No MeSH data available.


Related in: MedlinePlus