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Effect of da-cheng-qi decoction on the repair of the injured enteric nerve-interstitial cells of cajal-smooth muscle cells network in multiple organ dysfunction syndrome.

Liu MC, Xie MZ, Ma B, Qi QH - Evid Based Complement Alternat Med (2014)

Bottom Line: The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased.After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered.In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Liaoning 116011, China.

ABSTRACT
Wistar rats were randomly divided into control group, multiple organ dysfunction syndrome (MODS) group, and Da-Cheng-Qi decoction (DCQD) group. The network of enteric nerves-interstitial cells of Cajal- (ICC-) smooth muscle cells (SMC) in small intestine was observed using confocal laser scanning microscopy and transmission electron microscopy. The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased. The network integrity of enteric nerves-ICC-SMC was disrupted. The ultrastructures of ICC-DMP, enteric nerves, and SMC were severely damaged. After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered. In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions. The therapeutic mechanism of DCQD in part may be that it could repair the damages and maintain the integrity of enteric nerves ICC-SMC network.

No MeSH data available.


Related in: MedlinePlus

Ultrastructures of ICC-DMP using conventional electron microscopy. (a) Control group shows there were abundant mitochondria (☆), smooth endoplasmic reticulum, well-developed Golgi apparatus (↑), and intact basal membrane (♂) (ruler: 500 nm); (b) MODS group shows nucleus shriveled and the number of organelles decreased significantly. Also, mitochondria (☆) were distorting and swelling, and Golgi apparatus (↑) was damaged. Moreover, basal membrane (♂) was incomplete (ruler: 500 nm); (c) DCQD group shows nucleus was normal. Also, mitochondria (☆) and Golgi apparatus (↑) were more than MODS group. Only a few mitochondria were swelling and a few endoplasmic reticula were dilated. Moreover, basal membrane (♂) was almost complete (ruler: 200 nm).
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fig9: Ultrastructures of ICC-DMP using conventional electron microscopy. (a) Control group shows there were abundant mitochondria (☆), smooth endoplasmic reticulum, well-developed Golgi apparatus (↑), and intact basal membrane (♂) (ruler: 500 nm); (b) MODS group shows nucleus shriveled and the number of organelles decreased significantly. Also, mitochondria (☆) were distorting and swelling, and Golgi apparatus (↑) was damaged. Moreover, basal membrane (♂) was incomplete (ruler: 500 nm); (c) DCQD group shows nucleus was normal. Also, mitochondria (☆) and Golgi apparatus (↑) were more than MODS group. Only a few mitochondria were swelling and a few endoplasmic reticula were dilated. Moreover, basal membrane (♂) was almost complete (ruler: 200 nm).

Mentions: Control group (Figure 9(a)) shows ICC were shuttle type, nucleus was big, and oval and chromatins structure was clear. There were abundant mitochondria, smooth endoplasmic reticulum, ribosomes, and few of rough endoplasmic reticulum in ICC, and Golgi apparatus were small. There was intact basal membrane.


Effect of da-cheng-qi decoction on the repair of the injured enteric nerve-interstitial cells of cajal-smooth muscle cells network in multiple organ dysfunction syndrome.

Liu MC, Xie MZ, Ma B, Qi QH - Evid Based Complement Alternat Med (2014)

Ultrastructures of ICC-DMP using conventional electron microscopy. (a) Control group shows there were abundant mitochondria (☆), smooth endoplasmic reticulum, well-developed Golgi apparatus (↑), and intact basal membrane (♂) (ruler: 500 nm); (b) MODS group shows nucleus shriveled and the number of organelles decreased significantly. Also, mitochondria (☆) were distorting and swelling, and Golgi apparatus (↑) was damaged. Moreover, basal membrane (♂) was incomplete (ruler: 500 nm); (c) DCQD group shows nucleus was normal. Also, mitochondria (☆) and Golgi apparatus (↑) were more than MODS group. Only a few mitochondria were swelling and a few endoplasmic reticula were dilated. Moreover, basal membrane (♂) was almost complete (ruler: 200 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4247919&req=5

fig9: Ultrastructures of ICC-DMP using conventional electron microscopy. (a) Control group shows there were abundant mitochondria (☆), smooth endoplasmic reticulum, well-developed Golgi apparatus (↑), and intact basal membrane (♂) (ruler: 500 nm); (b) MODS group shows nucleus shriveled and the number of organelles decreased significantly. Also, mitochondria (☆) were distorting and swelling, and Golgi apparatus (↑) was damaged. Moreover, basal membrane (♂) was incomplete (ruler: 500 nm); (c) DCQD group shows nucleus was normal. Also, mitochondria (☆) and Golgi apparatus (↑) were more than MODS group. Only a few mitochondria were swelling and a few endoplasmic reticula were dilated. Moreover, basal membrane (♂) was almost complete (ruler: 200 nm).
Mentions: Control group (Figure 9(a)) shows ICC were shuttle type, nucleus was big, and oval and chromatins structure was clear. There were abundant mitochondria, smooth endoplasmic reticulum, ribosomes, and few of rough endoplasmic reticulum in ICC, and Golgi apparatus were small. There was intact basal membrane.

Bottom Line: The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased.After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered.In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Liaoning 116011, China.

ABSTRACT
Wistar rats were randomly divided into control group, multiple organ dysfunction syndrome (MODS) group, and Da-Cheng-Qi decoction (DCQD) group. The network of enteric nerves-interstitial cells of Cajal- (ICC-) smooth muscle cells (SMC) in small intestine was observed using confocal laser scanning microscopy and transmission electron microscopy. The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased. The network integrity of enteric nerves-ICC-SMC was disrupted. The ultrastructures of ICC-DMP, enteric nerves, and SMC were severely damaged. After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered. In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions. The therapeutic mechanism of DCQD in part may be that it could repair the damages and maintain the integrity of enteric nerves ICC-SMC network.

No MeSH data available.


Related in: MedlinePlus