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Effect of da-cheng-qi decoction on the repair of the injured enteric nerve-interstitial cells of cajal-smooth muscle cells network in multiple organ dysfunction syndrome.

Liu MC, Xie MZ, Ma B, Qi QH - Evid Based Complement Alternat Med (2014)

Bottom Line: The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased.After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered.In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Liaoning 116011, China.

ABSTRACT
Wistar rats were randomly divided into control group, multiple organ dysfunction syndrome (MODS) group, and Da-Cheng-Qi decoction (DCQD) group. The network of enteric nerves-interstitial cells of Cajal- (ICC-) smooth muscle cells (SMC) in small intestine was observed using confocal laser scanning microscopy and transmission electron microscopy. The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased. The network integrity of enteric nerves-ICC-SMC was disrupted. The ultrastructures of ICC-DMP, enteric nerves, and SMC were severely damaged. After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered. In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions. The therapeutic mechanism of DCQD in part may be that it could repair the damages and maintain the integrity of enteric nerves ICC-SMC network.

No MeSH data available.


Related in: MedlinePlus

Cholinergic nerves-ICC network using confocal microscopy. (a) It shows cholinergic nerves-ICC network of control group (→: nerve fiber, ♂: ICC (ruler: 200 ξm); (b) it shows cholinergic nerves-ICC network of MODS group (→: nerve fiber, ♂: ICC). Nerve fibers and ICC were significantly reduced and the nerves-ICC network integrity was damaged (ruler: 200 ξm); (c) it shows cholinergic nerves-ICC network of DCQD group (→: nerve fiber, ♂: ICC). Nerve fibers and neuronal connections were more than MODS group, maintaining net structure (ruler: 200 ξm).
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fig5: Cholinergic nerves-ICC network using confocal microscopy. (a) It shows cholinergic nerves-ICC network of control group (→: nerve fiber, ♂: ICC (ruler: 200 ξm); (b) it shows cholinergic nerves-ICC network of MODS group (→: nerve fiber, ♂: ICC). Nerve fibers and ICC were significantly reduced and the nerves-ICC network integrity was damaged (ruler: 200 ξm); (c) it shows cholinergic nerves-ICC network of DCQD group (→: nerve fiber, ♂: ICC). Nerve fibers and neuronal connections were more than MODS group, maintaining net structure (ruler: 200 ξm).

Mentions: Control group (Figures 5(a) and 6(a)) shows the dense c-Kit-positive cellular network (ICC-DMP), located between the longitudinal and circular muscularis and at the innermost part of the circular muscularis, and intermingled with the myenteric plexus. Between the circular muscle fibers, there was abundance of VAChT/nNOS-positive nerve fibers and c-Kit-positive cellular network run parallel with the muscle fibers. We also found that the VAChT/nNOS-positive myenteric plexus was surrounded by a reticular network of c-Kit-positive ICC.


Effect of da-cheng-qi decoction on the repair of the injured enteric nerve-interstitial cells of cajal-smooth muscle cells network in multiple organ dysfunction syndrome.

Liu MC, Xie MZ, Ma B, Qi QH - Evid Based Complement Alternat Med (2014)

Cholinergic nerves-ICC network using confocal microscopy. (a) It shows cholinergic nerves-ICC network of control group (→: nerve fiber, ♂: ICC (ruler: 200 ξm); (b) it shows cholinergic nerves-ICC network of MODS group (→: nerve fiber, ♂: ICC). Nerve fibers and ICC were significantly reduced and the nerves-ICC network integrity was damaged (ruler: 200 ξm); (c) it shows cholinergic nerves-ICC network of DCQD group (→: nerve fiber, ♂: ICC). Nerve fibers and neuronal connections were more than MODS group, maintaining net structure (ruler: 200 ξm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4247919&req=5

fig5: Cholinergic nerves-ICC network using confocal microscopy. (a) It shows cholinergic nerves-ICC network of control group (→: nerve fiber, ♂: ICC (ruler: 200 ξm); (b) it shows cholinergic nerves-ICC network of MODS group (→: nerve fiber, ♂: ICC). Nerve fibers and ICC were significantly reduced and the nerves-ICC network integrity was damaged (ruler: 200 ξm); (c) it shows cholinergic nerves-ICC network of DCQD group (→: nerve fiber, ♂: ICC). Nerve fibers and neuronal connections were more than MODS group, maintaining net structure (ruler: 200 ξm).
Mentions: Control group (Figures 5(a) and 6(a)) shows the dense c-Kit-positive cellular network (ICC-DMP), located between the longitudinal and circular muscularis and at the innermost part of the circular muscularis, and intermingled with the myenteric plexus. Between the circular muscle fibers, there was abundance of VAChT/nNOS-positive nerve fibers and c-Kit-positive cellular network run parallel with the muscle fibers. We also found that the VAChT/nNOS-positive myenteric plexus was surrounded by a reticular network of c-Kit-positive ICC.

Bottom Line: The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased.After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered.In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Liaoning 116011, China.

ABSTRACT
Wistar rats were randomly divided into control group, multiple organ dysfunction syndrome (MODS) group, and Da-Cheng-Qi decoction (DCQD) group. The network of enteric nerves-interstitial cells of Cajal- (ICC-) smooth muscle cells (SMC) in small intestine was observed using confocal laser scanning microscopy and transmission electron microscopy. The results showed that the numbers of cholinergic/nitriergic nerves, and the deep muscular plexus of ICC (ICC-DMP) and connexin43 (Cx43) in small intestine with MODS were significantly decreased. The network integrity of enteric nerves-ICC-SMC was disrupted. The ultrastructures of ICC-DMP, enteric nerves, and SMC were severely damaged. After treatment with DCQD, the damages were repaired and the network integrity of enteric nerves ICC-SMC was significantly recovered. In conclusion, the pathogenesis of gastrointestinal motility dysfunction in MODS in part may be due to the damages to enteric nerves-ICC-SMC network and gap junctions. The therapeutic mechanism of DCQD in part may be that it could repair the damages and maintain the integrity of enteric nerves ICC-SMC network.

No MeSH data available.


Related in: MedlinePlus