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Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development.

Zhang B, Liu X, Zhao G, Mao X, Li A, Jing R - J Integr Plant Biol (2014)

Bottom Line: On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions.The results also suggest a potential role for wheat SBP-box genes in ear development.This study provides a significant beginning of functional analysis of SBP-box genes in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

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Subcellular localization of TaSPL17–green fluorescent protein (GFP) fusion proteins driven by the CaMV35S promoter The vector control (35S::GFP) and the fusion proteins (35S::TaSPL17–GFP) were introduced into onion epidermal cells and observed with a laser scanning confocal microscope. Images are in dark field (1, 4), bright field (2, 5), and combined (3, 6).
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fig07: Subcellular localization of TaSPL17–green fluorescent protein (GFP) fusion proteins driven by the CaMV35S promoter The vector control (35S::GFP) and the fusion proteins (35S::TaSPL17–GFP) were introduced into onion epidermal cells and observed with a laser scanning confocal microscope. Images are in dark field (1, 4), bright field (2, 5), and combined (3, 6).

Mentions: The deduced amino acid sequences of wheat SBP-box genes contained a putative bipartite NLS, a major characteristic of transcription factors. TaSPL17 protein was selected for validating the NLS experimentally. The recombinant construct of the TaSPL-green fluorescent protein (GFP) fusion gene was transiently expressed in living onion epidermal cells. As expected, TaSPL17-GFP protein (GFP) was localized in the cell nucleus (Figure 7).


Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development.

Zhang B, Liu X, Zhao G, Mao X, Li A, Jing R - J Integr Plant Biol (2014)

Subcellular localization of TaSPL17–green fluorescent protein (GFP) fusion proteins driven by the CaMV35S promoter The vector control (35S::GFP) and the fusion proteins (35S::TaSPL17–GFP) were introduced into onion epidermal cells and observed with a laser scanning confocal microscope. Images are in dark field (1, 4), bright field (2, 5), and combined (3, 6).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4239008&req=5

fig07: Subcellular localization of TaSPL17–green fluorescent protein (GFP) fusion proteins driven by the CaMV35S promoter The vector control (35S::GFP) and the fusion proteins (35S::TaSPL17–GFP) were introduced into onion epidermal cells and observed with a laser scanning confocal microscope. Images are in dark field (1, 4), bright field (2, 5), and combined (3, 6).
Mentions: The deduced amino acid sequences of wheat SBP-box genes contained a putative bipartite NLS, a major characteristic of transcription factors. TaSPL17 protein was selected for validating the NLS experimentally. The recombinant construct of the TaSPL-green fluorescent protein (GFP) fusion gene was transiently expressed in living onion epidermal cells. As expected, TaSPL17-GFP protein (GFP) was localized in the cell nucleus (Figure 7).

Bottom Line: On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions.The results also suggest a potential role for wheat SBP-box genes in ear development.This study provides a significant beginning of functional analysis of SBP-box genes in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Show MeSH