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Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development.

Zhang B, Liu X, Zhao G, Mao X, Li A, Jing R - J Integr Plant Biol (2014)

Bottom Line: On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions.The results also suggest a potential role for wheat SBP-box genes in ear development.This study provides a significant beginning of functional analysis of SBP-box genes in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

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Real-time quantitative polymerase chain reaction analysis of TaSPL17 (B), TaSPL20 (C), and TaSPL21 (D) in wheat tissues at different stages 4C, 6C, and GC, shoot apical meristem at the four leaf, six leaf, and green-turning stages; 0.5E, 2E, 3E, 6E, 8E, and 10E, ears with different lengths, ranging 0.5–10 cm; BE and FE, ears at late booting stage and flowering stage (A); 4G, 7G, 14G, and 21G, grains at 4, 7, 14, and 21 d after flowering. Bar: 2 × SE.
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fig06: Real-time quantitative polymerase chain reaction analysis of TaSPL17 (B), TaSPL20 (C), and TaSPL21 (D) in wheat tissues at different stages 4C, 6C, and GC, shoot apical meristem at the four leaf, six leaf, and green-turning stages; 0.5E, 2E, 3E, 6E, 8E, and 10E, ears with different lengths, ranging 0.5–10 cm; BE and FE, ears at late booting stage and flowering stage (A); 4G, 7G, 14G, and 21G, grains at 4, 7, 14, and 21 d after flowering. Bar: 2 × SE.

Mentions: In order to check the expression profiles obtained by semiquantitative RT–PCR, TaSPL17, TaSPL20, and TaSPL21 were chosen as examples for illustrating the reliability. Because TaSPL17/20/21 mainly expressed in the shoot apical meristem and the ear, those wheat tissues at different stages were used for real-time quantitative (q)PCR. The expression levels revealed by the real-time PCR and semiquantitative RT–PCR were consistent with each other, further confirming their response to ear development (Figure 6).


Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development.

Zhang B, Liu X, Zhao G, Mao X, Li A, Jing R - J Integr Plant Biol (2014)

Real-time quantitative polymerase chain reaction analysis of TaSPL17 (B), TaSPL20 (C), and TaSPL21 (D) in wheat tissues at different stages 4C, 6C, and GC, shoot apical meristem at the four leaf, six leaf, and green-turning stages; 0.5E, 2E, 3E, 6E, 8E, and 10E, ears with different lengths, ranging 0.5–10 cm; BE and FE, ears at late booting stage and flowering stage (A); 4G, 7G, 14G, and 21G, grains at 4, 7, 14, and 21 d after flowering. Bar: 2 × SE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4239008&req=5

fig06: Real-time quantitative polymerase chain reaction analysis of TaSPL17 (B), TaSPL20 (C), and TaSPL21 (D) in wheat tissues at different stages 4C, 6C, and GC, shoot apical meristem at the four leaf, six leaf, and green-turning stages; 0.5E, 2E, 3E, 6E, 8E, and 10E, ears with different lengths, ranging 0.5–10 cm; BE and FE, ears at late booting stage and flowering stage (A); 4G, 7G, 14G, and 21G, grains at 4, 7, 14, and 21 d after flowering. Bar: 2 × SE.
Mentions: In order to check the expression profiles obtained by semiquantitative RT–PCR, TaSPL17, TaSPL20, and TaSPL21 were chosen as examples for illustrating the reliability. Because TaSPL17/20/21 mainly expressed in the shoot apical meristem and the ear, those wheat tissues at different stages were used for real-time quantitative (q)PCR. The expression levels revealed by the real-time PCR and semiquantitative RT–PCR were consistent with each other, further confirming their response to ear development (Figure 6).

Bottom Line: On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions.The results also suggest a potential role for wheat SBP-box genes in ear development.This study provides a significant beginning of functional analysis of SBP-box genes in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Show MeSH