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Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii.

Mota LA, Roberto Neto J, Monteiro VG, Lobato CS, Oliveira MA, Cunha Md, D'Ávila H, Seabra SH, Bozza PT, DaMatta RA - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source.Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis.Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.

ABSTRACT
Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.

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: macrophages cultured with mouse serum (MS) have a different morphologyappearance, higher number of vesicle-like organelles and greater area.Macrophages cultured with foetal bovine serum (FBS) (A, C) or MS (B, D).Differential interference contrast microscopy (A, B), Bar = 40 μm. Scanningelectron microscopy (C, D), Bar = 20 μm. Many vesicles (black arrow) andvesicle contours (white arrow) in macrophages cultured with MS can be seen.Area of macrophages cultured with FBS (black bar) or MS (white bar) of threeindependent experiments (E). Asterisk means significantly different from valuesfor macrophages cultured with FBS as calculated by the Studentt test (p > 0.05).
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f01: : macrophages cultured with mouse serum (MS) have a different morphologyappearance, higher number of vesicle-like organelles and greater area.Macrophages cultured with foetal bovine serum (FBS) (A, C) or MS (B, D).Differential interference contrast microscopy (A, B), Bar = 40 μm. Scanningelectron microscopy (C, D), Bar = 20 μm. Many vesicles (black arrow) andvesicle contours (white arrow) in macrophages cultured with MS can be seen.Area of macrophages cultured with FBS (black bar) or MS (white bar) of threeindependent experiments (E). Asterisk means significantly different from valuesfor macrophages cultured with FBS as calculated by the Studentt test (p > 0.05).

Mentions: Culturing in MS induces the formation of LBs in peritoneal macrophages- Macrophage cultures containing with FBS (MoFBS) or MS (MoMS) resulted in morphologicaldifferences and a greater number of vesicle-like organelles in MoMS (Fig. 1A, B).The fibroblast-like appearance of MoMS was also confirmed by SEM (Fig. 1A, D). Some regions ofthe plasma membrane of MoMS had fewer protrusions and were more homogenous and smootherand revealed round contours that suggest the presence of vesicle-like organelles beneaththe membrane (Fig. 1D). MoMS occupiedsignificantly more area than MoFBS (Fig. 1E). MoMSand MoFBS presented similar levels of F4/80 antigen on their surface (not shown).


Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii.

Mota LA, Roberto Neto J, Monteiro VG, Lobato CS, Oliveira MA, Cunha Md, D'Ávila H, Seabra SH, Bozza PT, DaMatta RA - Mem. Inst. Oswaldo Cruz (2014)

: macrophages cultured with mouse serum (MS) have a different morphologyappearance, higher number of vesicle-like organelles and greater area.Macrophages cultured with foetal bovine serum (FBS) (A, C) or MS (B, D).Differential interference contrast microscopy (A, B), Bar = 40 μm. Scanningelectron microscopy (C, D), Bar = 20 μm. Many vesicles (black arrow) andvesicle contours (white arrow) in macrophages cultured with MS can be seen.Area of macrophages cultured with FBS (black bar) or MS (white bar) of threeindependent experiments (E). Asterisk means significantly different from valuesfor macrophages cultured with FBS as calculated by the Studentt test (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4238769&req=5

f01: : macrophages cultured with mouse serum (MS) have a different morphologyappearance, higher number of vesicle-like organelles and greater area.Macrophages cultured with foetal bovine serum (FBS) (A, C) or MS (B, D).Differential interference contrast microscopy (A, B), Bar = 40 μm. Scanningelectron microscopy (C, D), Bar = 20 μm. Many vesicles (black arrow) andvesicle contours (white arrow) in macrophages cultured with MS can be seen.Area of macrophages cultured with FBS (black bar) or MS (white bar) of threeindependent experiments (E). Asterisk means significantly different from valuesfor macrophages cultured with FBS as calculated by the Studentt test (p > 0.05).
Mentions: Culturing in MS induces the formation of LBs in peritoneal macrophages- Macrophage cultures containing with FBS (MoFBS) or MS (MoMS) resulted in morphologicaldifferences and a greater number of vesicle-like organelles in MoMS (Fig. 1A, B).The fibroblast-like appearance of MoMS was also confirmed by SEM (Fig. 1A, D). Some regions ofthe plasma membrane of MoMS had fewer protrusions and were more homogenous and smootherand revealed round contours that suggest the presence of vesicle-like organelles beneaththe membrane (Fig. 1D). MoMS occupiedsignificantly more area than MoFBS (Fig. 1E). MoMSand MoFBS presented similar levels of F4/80 antigen on their surface (not shown).

Bottom Line: LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source.Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis.Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.

ABSTRACT
Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.

Show MeSH
Related in: MedlinePlus