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Evaluation of hypoglycemic efficacy of tangningtongluo formula, a traditional Chinese Miao medicine, in two rodent animal models.

Cheng L, Meng XB, Lu S, Wang TT, Liu Y, Sun GB, Sun XB - J Diabetes Res (2014)

Bottom Line: After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test.Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue.Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving β-cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.

ABSTRACT
Traditional Chinese medicines largely lack adequate and scientifically rigorous evidence regarding efficacy and functional mechanisms. The present study was aimed to confirm the hypoglycemic effect of Tangningtongluo (TNTL) formula, a traditional Chinese Miao medicine, in two animal models: high-fat diet and streptozotocin- (STZ-) induced diabetic rats and C57BL/KsJ-db/db diabetic mice. After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test. Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue. Compared with diabetic controls, a 12-week TNTL treatment regimen (dosages = 0.9, 1.8, and 3.6 g/kg) in db/db mice significantly decreased fasting glucose and HbA1c. Additionally, oral glucose tolerance in TNTL-treated mice improved significantly, compared with diabetic mice receiving metformin. Finally, tissue histopathology and biochemical index evaluations revealed significant improvement in TNTL-treated mice. Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving β-cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.

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The pathological examination result of pancreatic islets in STZ-induced diabetic rats. (a) Pancreatic islet from a normal mouse. (b) Pancreatic islet from STZ-induced diabetic rats with no treatment, severe degranulation of most β-cells in this islet. (c) and (d) Pancreatic islet from STZ-induced rats treated with Met and Glic, respectively. (e) and (f) Pancreatic islet from STZ-induced rats treated with TNTL (1.26 and 0.63 g/kg b.w., resp.).
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fig2: The pathological examination result of pancreatic islets in STZ-induced diabetic rats. (a) Pancreatic islet from a normal mouse. (b) Pancreatic islet from STZ-induced diabetic rats with no treatment, severe degranulation of most β-cells in this islet. (c) and (d) Pancreatic islet from STZ-induced rats treated with Met and Glic, respectively. (e) and (f) Pancreatic islet from STZ-induced rats treated with TNTL (1.26 and 0.63 g/kg b.w., resp.).

Mentions: Figure 2 shows the pathology of STZ-induced DM rat pancreas after 4 weeks of intervention. Pathological features of the pancreas in Group I showed no histopathological changes in the architecture of normal islet cells (Figure 2(a)). The STZ-induced DM group rats exhibited islet degeneration and a definitive loss of β-cells (Figure 2(b)), whereas islet architecture was preserved in TNTL-treated rats (Figures 2(e) and 2(f), resp.), with less necrosis of β-cells. Thus, TNTL in the pancreas ameliorated STZ-induced destruction of β-cells and the degree of inflammation. As expected, gliclazide (37 mg/kg) treatment yielded a moderate protective effect.


Evaluation of hypoglycemic efficacy of tangningtongluo formula, a traditional Chinese Miao medicine, in two rodent animal models.

Cheng L, Meng XB, Lu S, Wang TT, Liu Y, Sun GB, Sun XB - J Diabetes Res (2014)

The pathological examination result of pancreatic islets in STZ-induced diabetic rats. (a) Pancreatic islet from a normal mouse. (b) Pancreatic islet from STZ-induced diabetic rats with no treatment, severe degranulation of most β-cells in this islet. (c) and (d) Pancreatic islet from STZ-induced rats treated with Met and Glic, respectively. (e) and (f) Pancreatic islet from STZ-induced rats treated with TNTL (1.26 and 0.63 g/kg b.w., resp.).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4238278&req=5

fig2: The pathological examination result of pancreatic islets in STZ-induced diabetic rats. (a) Pancreatic islet from a normal mouse. (b) Pancreatic islet from STZ-induced diabetic rats with no treatment, severe degranulation of most β-cells in this islet. (c) and (d) Pancreatic islet from STZ-induced rats treated with Met and Glic, respectively. (e) and (f) Pancreatic islet from STZ-induced rats treated with TNTL (1.26 and 0.63 g/kg b.w., resp.).
Mentions: Figure 2 shows the pathology of STZ-induced DM rat pancreas after 4 weeks of intervention. Pathological features of the pancreas in Group I showed no histopathological changes in the architecture of normal islet cells (Figure 2(a)). The STZ-induced DM group rats exhibited islet degeneration and a definitive loss of β-cells (Figure 2(b)), whereas islet architecture was preserved in TNTL-treated rats (Figures 2(e) and 2(f), resp.), with less necrosis of β-cells. Thus, TNTL in the pancreas ameliorated STZ-induced destruction of β-cells and the degree of inflammation. As expected, gliclazide (37 mg/kg) treatment yielded a moderate protective effect.

Bottom Line: After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test.Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue.Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving β-cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.

ABSTRACT
Traditional Chinese medicines largely lack adequate and scientifically rigorous evidence regarding efficacy and functional mechanisms. The present study was aimed to confirm the hypoglycemic effect of Tangningtongluo (TNTL) formula, a traditional Chinese Miao medicine, in two animal models: high-fat diet and streptozotocin- (STZ-) induced diabetic rats and C57BL/KsJ-db/db diabetic mice. After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test. Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue. Compared with diabetic controls, a 12-week TNTL treatment regimen (dosages = 0.9, 1.8, and 3.6 g/kg) in db/db mice significantly decreased fasting glucose and HbA1c. Additionally, oral glucose tolerance in TNTL-treated mice improved significantly, compared with diabetic mice receiving metformin. Finally, tissue histopathology and biochemical index evaluations revealed significant improvement in TNTL-treated mice. Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving β-cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.

Show MeSH
Related in: MedlinePlus