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Consequences of lethal-whole-body gamma radiation and possible ameliorative role of melatonin.

Mihandoost E, Shirazi A, Mahdavi SR, Aliasgharzadeh A - ScientificWorldJournal (2014)

Bottom Line: Melatonin synergistically acts as an immune-stimulator and antioxidant.The melatonin pretreated irradiated rats showed less change in body weight as compared to radiation only group.On the other hand, melatonin appeared to have another radioprotective role, suggesting that melatonin may reduce apoptosis through a caspase-3-mediated pathway by blocking caspase-3 activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT
Gamma radiation induces the generation of free radicals, leading to serious cellular damages in biological systems. Radioprotectors act as prophylactic agents that are administered to shield normal cells and tissues from the deleterious effects of radiation. Melatonin synergistically acts as an immune-stimulator and antioxidant. We investigated the possible radioprotective role of melatonin (100 mg/kg i.p.) against lethal-whole-body radiation- (10 Gy) induced sickness, body weight loss, and mortality in rats. Results of the present study suggest that exposure to lethal-whole-body radiation incurred mortality, body weight loss, and apoptosis and it also depleted the immunity and the antioxidant status of the rats. Our results show that melatonin pretreatment provides protection against radiation induced mortality, oxidative stress, and immune-suppression. The melatonin pretreated irradiated rats showed less change in body weight as compared to radiation only group. On the other hand, melatonin appeared to have another radioprotective role, suggesting that melatonin may reduce apoptosis through a caspase-3-mediated pathway by blocking caspase-3 activity.

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Related in: MedlinePlus

Percent change in body weight of rats. Con: Control group, Veh: Vehicle only group, Mel: Melatonin only group, Rad: Radiation only group, Veh + Rad: Treated with vehicle and exposed to 10 Gy radiation, and Mel + Rad: Treated with 100 mg/kg melatonin before exposure to 10 Gy radiation.
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fig2: Percent change in body weight of rats. Con: Control group, Veh: Vehicle only group, Mel: Melatonin only group, Rad: Radiation only group, Veh + Rad: Treated with vehicle and exposed to 10 Gy radiation, and Mel + Rad: Treated with 100 mg/kg melatonin before exposure to 10 Gy radiation.

Mentions: Figures 1 and 2 show the results of survival and body weight studies. The NO and MDA levels, antioxidant enzymes activities and total antioxidant capacity (TAC) level, lymphocytes count (LC), and caspase-3 activity are given in Figures 3–10.


Consequences of lethal-whole-body gamma radiation and possible ameliorative role of melatonin.

Mihandoost E, Shirazi A, Mahdavi SR, Aliasgharzadeh A - ScientificWorldJournal (2014)

Percent change in body weight of rats. Con: Control group, Veh: Vehicle only group, Mel: Melatonin only group, Rad: Radiation only group, Veh + Rad: Treated with vehicle and exposed to 10 Gy radiation, and Mel + Rad: Treated with 100 mg/kg melatonin before exposure to 10 Gy radiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4238234&req=5

fig2: Percent change in body weight of rats. Con: Control group, Veh: Vehicle only group, Mel: Melatonin only group, Rad: Radiation only group, Veh + Rad: Treated with vehicle and exposed to 10 Gy radiation, and Mel + Rad: Treated with 100 mg/kg melatonin before exposure to 10 Gy radiation.
Mentions: Figures 1 and 2 show the results of survival and body weight studies. The NO and MDA levels, antioxidant enzymes activities and total antioxidant capacity (TAC) level, lymphocytes count (LC), and caspase-3 activity are given in Figures 3–10.

Bottom Line: Melatonin synergistically acts as an immune-stimulator and antioxidant.The melatonin pretreated irradiated rats showed less change in body weight as compared to radiation only group.On the other hand, melatonin appeared to have another radioprotective role, suggesting that melatonin may reduce apoptosis through a caspase-3-mediated pathway by blocking caspase-3 activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT
Gamma radiation induces the generation of free radicals, leading to serious cellular damages in biological systems. Radioprotectors act as prophylactic agents that are administered to shield normal cells and tissues from the deleterious effects of radiation. Melatonin synergistically acts as an immune-stimulator and antioxidant. We investigated the possible radioprotective role of melatonin (100 mg/kg i.p.) against lethal-whole-body radiation- (10 Gy) induced sickness, body weight loss, and mortality in rats. Results of the present study suggest that exposure to lethal-whole-body radiation incurred mortality, body weight loss, and apoptosis and it also depleted the immunity and the antioxidant status of the rats. Our results show that melatonin pretreatment provides protection against radiation induced mortality, oxidative stress, and immune-suppression. The melatonin pretreated irradiated rats showed less change in body weight as compared to radiation only group. On the other hand, melatonin appeared to have another radioprotective role, suggesting that melatonin may reduce apoptosis through a caspase-3-mediated pathway by blocking caspase-3 activity.

Show MeSH
Related in: MedlinePlus