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Therapeutic effect of bone marrow mesenchymal stem cells on cold stress induced changes in the hippocampus of rats.

Kumar SK, Perumal S, Rajagopalan V - Neural Regen Res (2014)

Bottom Line: The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold stress induced neuronal changes in hippocampal CA1 region of Wistar rats.Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA1 region.Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Saveetha Dental College, Chennai, India.

ABSTRACT
The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold stress induced neuronal changes in hippocampal CA1 region of Wistar rats. Bone marrow mesenchymal stem cells were isolated from a 6-week-old Wistar rat. Bone marrow from adult femora and tibia was collected and mesenchymal stem cells were cultured in minimal essential medium containing 10% heat-inactivated fetal bovine serum and were sub-cultured. Passage 3 cells were analyzed by flow cytometry for positive expression of CD44 and CD90 and negative expression of CD45. Once CD44 and CD90 positive expression was achieved, the cells were cultured again to 90% confluence for later experiments. Twenty-four rats aged 8 weeks old were randomly and evenly divided into normal control, cold water swim stress (cold stress), cold stress + PBS (intravenous infusion), and cold stress + bone marrow mesenchymal stem cells (1 × 10(6); intravenous infusion) groups. The total period of study was 60 days which included 1 month stress period followed by 1 month treatment. Behavioral functional test was performed during the entire study period. After treatment, rats were sacrificed for histological studies. Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA1 region. Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

No MeSH data available.


Related in: MedlinePlus

Identification and morphology of rat bone marrow mesenchymal stem cells.(A; 40 ×) A mixed population of both hematopoietic and mesenchymal stem cells (MSCs) on the 3rd day after culture. Arrow indicates rat MSCs. (B; 200 ×) Change in morphology of MSCs on the 5th day after culture. Long arrow indicates triangular cells and short arrow indicates star-shaped cells. (C; 40 ×) Homogenous population of MSCs showing spindle-shaped morphology on the 14th day after culture.
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Figure 1: Identification and morphology of rat bone marrow mesenchymal stem cells.(A; 40 ×) A mixed population of both hematopoietic and mesenchymal stem cells (MSCs) on the 3rd day after culture. Arrow indicates rat MSCs. (B; 200 ×) Change in morphology of MSCs on the 5th day after culture. Long arrow indicates triangular cells and short arrow indicates star-shaped cells. (C; 40 ×) Homogenous population of MSCs showing spindle-shaped morphology on the 14th day after culture.

Mentions: On the 3rd day of culture, we observed the mixed population of cells, and only a few MSCs were present. The MSCs showed a tendency to adhere to the tissue culture plastic and were triangular-shaped with eccentric nucleus. Other mixed cells (Figure 1A) were round. The ratio between MSCs and other mixed cell populations was small. On the 5th day of the culture, MSCs had started to grow with their long processes extended (Figure 1B) among the mixed cells. Compared to the 3rd day, there were more MSCs. On the 8th day, clusters of MSCs with long processes, one nucleus and one nucleolus were present. The MSCs had gradually changed from triangular to star-shaped then to spindle-shaped. During the subculture study, homogeneous adult MSCs were obtained. On the 14th day, multiple colonies of MSCs were present, which were thickly populated. There was a linear increase in the number of colonies and mesenhymal stem cells (Figure 1C). Viability of cells determined by Trypan blue dye reveals that about 99% of the cells were viable.


Therapeutic effect of bone marrow mesenchymal stem cells on cold stress induced changes in the hippocampus of rats.

Kumar SK, Perumal S, Rajagopalan V - Neural Regen Res (2014)

Identification and morphology of rat bone marrow mesenchymal stem cells.(A; 40 ×) A mixed population of both hematopoietic and mesenchymal stem cells (MSCs) on the 3rd day after culture. Arrow indicates rat MSCs. (B; 200 ×) Change in morphology of MSCs on the 5th day after culture. Long arrow indicates triangular cells and short arrow indicates star-shaped cells. (C; 40 ×) Homogenous population of MSCs showing spindle-shaped morphology on the 14th day after culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4238161&req=5

Figure 1: Identification and morphology of rat bone marrow mesenchymal stem cells.(A; 40 ×) A mixed population of both hematopoietic and mesenchymal stem cells (MSCs) on the 3rd day after culture. Arrow indicates rat MSCs. (B; 200 ×) Change in morphology of MSCs on the 5th day after culture. Long arrow indicates triangular cells and short arrow indicates star-shaped cells. (C; 40 ×) Homogenous population of MSCs showing spindle-shaped morphology on the 14th day after culture.
Mentions: On the 3rd day of culture, we observed the mixed population of cells, and only a few MSCs were present. The MSCs showed a tendency to adhere to the tissue culture plastic and were triangular-shaped with eccentric nucleus. Other mixed cells (Figure 1A) were round. The ratio between MSCs and other mixed cell populations was small. On the 5th day of the culture, MSCs had started to grow with their long processes extended (Figure 1B) among the mixed cells. Compared to the 3rd day, there were more MSCs. On the 8th day, clusters of MSCs with long processes, one nucleus and one nucleolus were present. The MSCs had gradually changed from triangular to star-shaped then to spindle-shaped. During the subculture study, homogeneous adult MSCs were obtained. On the 14th day, multiple colonies of MSCs were present, which were thickly populated. There was a linear increase in the number of colonies and mesenhymal stem cells (Figure 1C). Viability of cells determined by Trypan blue dye reveals that about 99% of the cells were viable.

Bottom Line: The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold stress induced neuronal changes in hippocampal CA1 region of Wistar rats.Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA1 region.Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Saveetha Dental College, Chennai, India.

ABSTRACT
The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold stress induced neuronal changes in hippocampal CA1 region of Wistar rats. Bone marrow mesenchymal stem cells were isolated from a 6-week-old Wistar rat. Bone marrow from adult femora and tibia was collected and mesenchymal stem cells were cultured in minimal essential medium containing 10% heat-inactivated fetal bovine serum and were sub-cultured. Passage 3 cells were analyzed by flow cytometry for positive expression of CD44 and CD90 and negative expression of CD45. Once CD44 and CD90 positive expression was achieved, the cells were cultured again to 90% confluence for later experiments. Twenty-four rats aged 8 weeks old were randomly and evenly divided into normal control, cold water swim stress (cold stress), cold stress + PBS (intravenous infusion), and cold stress + bone marrow mesenchymal stem cells (1 × 10(6); intravenous infusion) groups. The total period of study was 60 days which included 1 month stress period followed by 1 month treatment. Behavioral functional test was performed during the entire study period. After treatment, rats were sacrificed for histological studies. Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA1 region. Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

No MeSH data available.


Related in: MedlinePlus