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Evidence for a 12 kDa "Carrier Protein" for Natriuretic Hormone.

Gonick HC - Front Endocrinol (Lausanne) (2014)

Bottom Line: The carrier protein has a molecular weight of 12 kDa while the final hormone has a molecular weight of 408 Da.Both compounds inhibit Na-K-ATPase but the compound containing the carrier protein predominates.The question has been raised as to whether the carrier protein is in actuality proANF, a 17 kDa protein that can be split between a 14 kDa protein (the presumptive proANF) and the 3 kDa ANF.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles , Los Angeles, CA , USA.

ABSTRACT
The search for the elusive Na-K-ATPase-inhibiting natriuretic hormone continues. In this review, evidence is presented that isolating the carrier protein for natriuretic hormone from hypertensive plasma is a necessary first step before splitting off the final hormone. The carrier protein has a molecular weight of 12 kDa while the final hormone has a molecular weight of 408 Da. Both compounds inhibit Na-K-ATPase but the compound containing the carrier protein predominates. The question has been raised as to whether the carrier protein is in actuality proANF, a 17 kDa protein that can be split between a 14 kDa protein (the presumptive proANF) and the 3 kDa ANF.

No MeSH data available.


Relative optical densities of 12 and 21 kDa bands. *p < 0.05 for CRF, Aldo, EHT compared to normals < 50 and for CHF compared to normals > 50. From Ref. (14) with permission.
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Figure 5: Relative optical densities of 12 and 21 kDa bands. *p < 0.05 for CRF, Aldo, EHT compared to normals < 50 and for CHF compared to normals > 50. From Ref. (14) with permission.

Mentions: In a third study (15), plasma from 101 patients were examined [25 normals (N) < age 50, 13N > age 50, 7 with acute CHF, 24 with chronic renal failure (CRF), on dialysis, 5 with idiopathic hyperaldosteronism (PA), and 27 with essential hypertension, untreated (EHT)]. Plasma was extracted with 32% acetonitrile, and analyzed by fluoroimmunoassay (DELFIA) for marinobufagenin and ouabain. In addition, from 32 patients (6N < 50, 6N > 50, 5 CHF, 5 CRF, 6 EHT, and 4 PA), SDS gradient gels were obtained. The 12 kDa bands were extracted, analyzed for Na-K-ATPase inhibition, marinobufagenin, and ouabain, and compared to 14 and 21 kDa bands. Marinobufagenin was found to be elevated in CRF, EHT, PA, and CHF. Ouabain was increased only in PA. When the relative optical densities of 12 and 21 kDa bands were contrasted, CRF, PA, and HT were found to be increased and CHF to be decreased in the 12 kDa band, with no discernible changes in the 21 kDa bands (Figure 5). Following extraction of the bands, Na-K-ATPase inhibitory activity measured 38% in 16 pooled 12 kDa bands, with essentially no activity found in the 14 kDa or 21 kDa bands. SDS-PAGE separation of plasma proteins confirmed that the 12 kDa band was elevated in primary aldosteronism, diminished in CHF, with return toward normal after treatment (Figure 6). Thus, only the 12 kDa band possessed all of the attributes of natriuretic hormone.


Evidence for a 12 kDa "Carrier Protein" for Natriuretic Hormone.

Gonick HC - Front Endocrinol (Lausanne) (2014)

Relative optical densities of 12 and 21 kDa bands. *p < 0.05 for CRF, Aldo, EHT compared to normals < 50 and for CHF compared to normals > 50. From Ref. (14) with permission.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4237141&req=5

Figure 5: Relative optical densities of 12 and 21 kDa bands. *p < 0.05 for CRF, Aldo, EHT compared to normals < 50 and for CHF compared to normals > 50. From Ref. (14) with permission.
Mentions: In a third study (15), plasma from 101 patients were examined [25 normals (N) < age 50, 13N > age 50, 7 with acute CHF, 24 with chronic renal failure (CRF), on dialysis, 5 with idiopathic hyperaldosteronism (PA), and 27 with essential hypertension, untreated (EHT)]. Plasma was extracted with 32% acetonitrile, and analyzed by fluoroimmunoassay (DELFIA) for marinobufagenin and ouabain. In addition, from 32 patients (6N < 50, 6N > 50, 5 CHF, 5 CRF, 6 EHT, and 4 PA), SDS gradient gels were obtained. The 12 kDa bands were extracted, analyzed for Na-K-ATPase inhibition, marinobufagenin, and ouabain, and compared to 14 and 21 kDa bands. Marinobufagenin was found to be elevated in CRF, EHT, PA, and CHF. Ouabain was increased only in PA. When the relative optical densities of 12 and 21 kDa bands were contrasted, CRF, PA, and HT were found to be increased and CHF to be decreased in the 12 kDa band, with no discernible changes in the 21 kDa bands (Figure 5). Following extraction of the bands, Na-K-ATPase inhibitory activity measured 38% in 16 pooled 12 kDa bands, with essentially no activity found in the 14 kDa or 21 kDa bands. SDS-PAGE separation of plasma proteins confirmed that the 12 kDa band was elevated in primary aldosteronism, diminished in CHF, with return toward normal after treatment (Figure 6). Thus, only the 12 kDa band possessed all of the attributes of natriuretic hormone.

Bottom Line: The carrier protein has a molecular weight of 12 kDa while the final hormone has a molecular weight of 408 Da.Both compounds inhibit Na-K-ATPase but the compound containing the carrier protein predominates.The question has been raised as to whether the carrier protein is in actuality proANF, a 17 kDa protein that can be split between a 14 kDa protein (the presumptive proANF) and the 3 kDa ANF.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles , Los Angeles, CA , USA.

ABSTRACT
The search for the elusive Na-K-ATPase-inhibiting natriuretic hormone continues. In this review, evidence is presented that isolating the carrier protein for natriuretic hormone from hypertensive plasma is a necessary first step before splitting off the final hormone. The carrier protein has a molecular weight of 12 kDa while the final hormone has a molecular weight of 408 Da. Both compounds inhibit Na-K-ATPase but the compound containing the carrier protein predominates. The question has been raised as to whether the carrier protein is in actuality proANF, a 17 kDa protein that can be split between a 14 kDa protein (the presumptive proANF) and the 3 kDa ANF.

No MeSH data available.