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Protein-protein interaction network and mechanism analysis in ischemic stroke.

Quan Z, Quan Y, Wei B, Fang D, Yu W, Jia H, Quan W, Liu Y, Wang Q - Mol Med Rep (2014)

Bottom Line: Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time.The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples.In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong 250000, P.R. China.

ABSTRACT
Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)-induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO-induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein-protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin-6 (IL-6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke.

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Related in: MedlinePlus

Venn diagram demonstrating that at one (green), three (orange) and seven (blue) days following MCAO, the samples have overlapping but distinct sets of DEGs. A total of 337 DEGs are identified in the MCAO samples, including 227 distinct DEGs in samples obtained one day following MCAO, 27 distinct DEGs in samples obtained three days following MCAO and 14 distinct DEGs obtained seven days following MCAO. DEGs, differentially expressed genes; MCAO, middle cerebral artery occlusion.
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f1-mmr-11-01-0029: Venn diagram demonstrating that at one (green), three (orange) and seven (blue) days following MCAO, the samples have overlapping but distinct sets of DEGs. A total of 337 DEGs are identified in the MCAO samples, including 227 distinct DEGs in samples obtained one day following MCAO, 27 distinct DEGs in samples obtained three days following MCAO and 14 distinct DEGs obtained seven days following MCAO. DEGs, differentially expressed genes; MCAO, middle cerebral artery occlusion.

Mentions: In order to obtain the DEGs between MCAO reactive astrocytes and sham controls at various time points, publically available microarray datasets were obtained from the GEO. A total of 294 genes were selected as DEGs between samples obtained from one day following MCAO and sham specimens; 87 DEGs between samples from three days following MCAO and sham samples; and 57 DEGs between samples from seven days following MCAO and sham controls with a fold-change >1.5 and P<0.05. The samples obtained from one, three and seven days following MCAO had overlapping but distinct sets of DEGs. The Venn diagram (Fig. 1) demonstrates that 32 genes are common to the three MCAO samples and all of these genes were upregulated in the MCAO-reactive astrocytes. There were 227, 27 and 14 distinct DEGs in the samples taken from one, three and seven days following MCAO, respectively.


Protein-protein interaction network and mechanism analysis in ischemic stroke.

Quan Z, Quan Y, Wei B, Fang D, Yu W, Jia H, Quan W, Liu Y, Wang Q - Mol Med Rep (2014)

Venn diagram demonstrating that at one (green), three (orange) and seven (blue) days following MCAO, the samples have overlapping but distinct sets of DEGs. A total of 337 DEGs are identified in the MCAO samples, including 227 distinct DEGs in samples obtained one day following MCAO, 27 distinct DEGs in samples obtained three days following MCAO and 14 distinct DEGs obtained seven days following MCAO. DEGs, differentially expressed genes; MCAO, middle cerebral artery occlusion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4237100&req=5

f1-mmr-11-01-0029: Venn diagram demonstrating that at one (green), three (orange) and seven (blue) days following MCAO, the samples have overlapping but distinct sets of DEGs. A total of 337 DEGs are identified in the MCAO samples, including 227 distinct DEGs in samples obtained one day following MCAO, 27 distinct DEGs in samples obtained three days following MCAO and 14 distinct DEGs obtained seven days following MCAO. DEGs, differentially expressed genes; MCAO, middle cerebral artery occlusion.
Mentions: In order to obtain the DEGs between MCAO reactive astrocytes and sham controls at various time points, publically available microarray datasets were obtained from the GEO. A total of 294 genes were selected as DEGs between samples obtained from one day following MCAO and sham specimens; 87 DEGs between samples from three days following MCAO and sham samples; and 57 DEGs between samples from seven days following MCAO and sham controls with a fold-change >1.5 and P<0.05. The samples obtained from one, three and seven days following MCAO had overlapping but distinct sets of DEGs. The Venn diagram (Fig. 1) demonstrates that 32 genes are common to the three MCAO samples and all of these genes were upregulated in the MCAO-reactive astrocytes. There were 227, 27 and 14 distinct DEGs in the samples taken from one, three and seven days following MCAO, respectively.

Bottom Line: Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time.The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples.In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong 250000, P.R. China.

ABSTRACT
Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)-induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO-induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein-protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin-6 (IL-6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke.

Show MeSH
Related in: MedlinePlus