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Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease.

Liao SX, Ding T, Rao XM, Sun DS, Sun PP, Wang YJ, Fu DD, Liu XL, Ou-Yang Y - Mol Med Rep (2014)

Bottom Line: Using RT-qPCR, the expression levels of CCR7+ and CD83+ mRNA were found to be reduced in the smokers with COPD as compared with the non-smokers without COPD group (P<0.05, respectively).Cigarette smoke may stimulate immune responses by impairing the homing of airway DCs to the lymph nodes and reduce the migratory potential of DCs.The present study revealed that COPD is associated with reduced numbers of mature CD83+ DCs and lower CCR7+ expression levels in small airways.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.

ABSTRACT
The aim of the present study was to characterize and quantify the numbers and expression levels of cells markers associated with dendritic cell (DC) maturation in small airways in current smokers and non-smokers with or without chronic obstructive pulmonary disease (COPD). Lung tissues from the following 32 patients were obtained during resection for lung cancer: Eight smokers with COPD, eight non-smokers with COPD, eight current smokers without COPD and eight non-smokers without COPD, serving as a control. The tissue sections were immunostained for cluster of differentiation (CD)83+ and CD1a+ to delineate mature and immature DCs, and chemokine receptor type 7 (CCR7+) to detect DC migratory ability. Myeloid DCs were collected from the lung tissues, and subsequently the CD83+ and CCR7+ expression levels in the lung myeloid DCs were detected using flow cytometry. The expression levels of CD83+, CD1a+ and CCR7+ mRNA in total lung RNA were evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Evident chronic bronchitis and emphysema pathological changes were observed in the lung tissues of patients with COPD. The results revealed that the numbers of CD83+ and CCR7+ DCs were reduced but the numbers of CD1a+ DCs were significantly increased in the COPD group as compared with the control group (P<0.05, respectively). Using RT-qPCR, the expression levels of CCR7+ and CD83+ mRNA were found to be reduced in the smokers with COPD as compared with the non-smokers without COPD group (P<0.05, respectively). Excessive local adaptive immune responses are key elements in the pathogenesis of COPD. Cigarette smoke may stimulate immune responses by impairing the homing of airway DCs to the lymph nodes and reduce the migratory potential of DCs. The present study revealed that COPD is associated with reduced numbers of mature CD83+ DCs and lower CCR7+ expression levels in small airways.

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Related in: MedlinePlus

CD83+ and CD1a+ cells in lung tissue specimens from each group of patients. Data are presented as the mean ± standard deviation, n=8. *P<0.05 and **P<0.01 compared with the control group (CS−COPD−). CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS−COPD−, non-smoker without COPD; CS−COPD+, non-smoker with COPD; CS+COPD−, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.
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f4-mmr-11-01-0219: CD83+ and CD1a+ cells in lung tissue specimens from each group of patients. Data are presented as the mean ± standard deviation, n=8. *P<0.05 and **P<0.01 compared with the control group (CS−COPD−). CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS−COPD−, non-smoker without COPD; CS−COPD+, non-smoker with COPD; CS+COPD−, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.

Mentions: Immunohistochemical analysis of CD83+, CD1a+ and CCR7+ DCs revealed that a greater number of CD1a+ DCs were detected in virtually all smoker and patients with COPD, whereas CD83+ and CCR7+ were specific for the control, asymptomatic non-smoker group (Fig. 3). The results of the cell number count (Fig. 4) demonstrated that the numbers of CD83+ and CCR7+ DCs were significantly reduced, but the numbers of CD1a+ DCs were significantly increased in the COPD and smoker groups as compared with the control group (P<0.05).


Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease.

Liao SX, Ding T, Rao XM, Sun DS, Sun PP, Wang YJ, Fu DD, Liu XL, Ou-Yang Y - Mol Med Rep (2014)

CD83+ and CD1a+ cells in lung tissue specimens from each group of patients. Data are presented as the mean ± standard deviation, n=8. *P<0.05 and **P<0.01 compared with the control group (CS−COPD−). CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS−COPD−, non-smoker without COPD; CS−COPD+, non-smoker with COPD; CS+COPD−, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4237095&req=5

f4-mmr-11-01-0219: CD83+ and CD1a+ cells in lung tissue specimens from each group of patients. Data are presented as the mean ± standard deviation, n=8. *P<0.05 and **P<0.01 compared with the control group (CS−COPD−). CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS−COPD−, non-smoker without COPD; CS−COPD+, non-smoker with COPD; CS+COPD−, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.
Mentions: Immunohistochemical analysis of CD83+, CD1a+ and CCR7+ DCs revealed that a greater number of CD1a+ DCs were detected in virtually all smoker and patients with COPD, whereas CD83+ and CCR7+ were specific for the control, asymptomatic non-smoker group (Fig. 3). The results of the cell number count (Fig. 4) demonstrated that the numbers of CD83+ and CCR7+ DCs were significantly reduced, but the numbers of CD1a+ DCs were significantly increased in the COPD and smoker groups as compared with the control group (P<0.05).

Bottom Line: Using RT-qPCR, the expression levels of CCR7+ and CD83+ mRNA were found to be reduced in the smokers with COPD as compared with the non-smokers without COPD group (P<0.05, respectively).Cigarette smoke may stimulate immune responses by impairing the homing of airway DCs to the lymph nodes and reduce the migratory potential of DCs.The present study revealed that COPD is associated with reduced numbers of mature CD83+ DCs and lower CCR7+ expression levels in small airways.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.

ABSTRACT
The aim of the present study was to characterize and quantify the numbers and expression levels of cells markers associated with dendritic cell (DC) maturation in small airways in current smokers and non-smokers with or without chronic obstructive pulmonary disease (COPD). Lung tissues from the following 32 patients were obtained during resection for lung cancer: Eight smokers with COPD, eight non-smokers with COPD, eight current smokers without COPD and eight non-smokers without COPD, serving as a control. The tissue sections were immunostained for cluster of differentiation (CD)83+ and CD1a+ to delineate mature and immature DCs, and chemokine receptor type 7 (CCR7+) to detect DC migratory ability. Myeloid DCs were collected from the lung tissues, and subsequently the CD83+ and CCR7+ expression levels in the lung myeloid DCs were detected using flow cytometry. The expression levels of CD83+, CD1a+ and CCR7+ mRNA in total lung RNA were evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Evident chronic bronchitis and emphysema pathological changes were observed in the lung tissues of patients with COPD. The results revealed that the numbers of CD83+ and CCR7+ DCs were reduced but the numbers of CD1a+ DCs were significantly increased in the COPD group as compared with the control group (P<0.05, respectively). Using RT-qPCR, the expression levels of CCR7+ and CD83+ mRNA were found to be reduced in the smokers with COPD as compared with the non-smokers without COPD group (P<0.05, respectively). Excessive local adaptive immune responses are key elements in the pathogenesis of COPD. Cigarette smoke may stimulate immune responses by impairing the homing of airway DCs to the lymph nodes and reduce the migratory potential of DCs. The present study revealed that COPD is associated with reduced numbers of mature CD83+ DCs and lower CCR7+ expression levels in small airways.

Show MeSH
Related in: MedlinePlus