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Analysis of metabolic characteristics in a rat model of chronic pancreatitis using high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy.

Tian B, Ma C, Wang J, Pan CS, Yang GJ, Lu JP - Mol Med Rep (2014)

Bottom Line: The metabolite betaine positively correlated with fibrosis and inflammatory cell infiltration in CP.In addition, aspartate negatively correlated with fibrosis, but exhibited no significant correlation with inflammatory cell infiltration.Furthermore, the presence of fatty acids negatively correlated with fibrosis and inflammatory cell infiltration in CP.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Changhai Hospital of Shanghai, The Second Military Medical University, Shanghai 200433, P.R. China.

ABSTRACT
Pathological and metabolic alterations co-exist and co-develop in the progression of chronic pancreatitis (CP). The aim of the present study was to investigate the metabolic characteristics and disease severity of a rat model of CP in order to determine associations in the observed pathology and the metabolites of CP using high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR). Wistar rats (n=36) were randomly assigned into 6 groups (n=6 per group). CP was established by administering dibutyltin dichloride solution into the tail vein. After 0, 7, 14, 21, 28 and 35 days, the pancreatic tissues were collected for pathological scoring or for HR-MAS NMR. Correlation analyses between the major pathological scores and the integral areas of the major metabolites were determined. The most representative metabolites, aspartate, betaine and fatty acids, were identified as possessing the greatest discriminatory significance. The Spearman's rank correlation coefficients between the pathology and metabolites of the pancreatic tissues were as follows: Betaine and fibrosis, 0.454 (P=0.044); betaine and inflammatory cell infiltration, 0.716 (P=0.0001); aspartate and fibrosis, -0.768 (P=0.0001); aspartate and inflammatory cell infiltration, -0.394 (P=0.085); fatty acid and fibrosis, -0.764 (P=0.0001); and fatty acid and inflammatory cell infiltration, -0.619 (P=0.004). The metabolite betaine positively correlated with fibrosis and inflammatory cell infiltration in CP. In addition, aspartate negatively correlated with fibrosis, but exhibited no significant correlation with inflammatory cell infiltration. Furthermore, the presence of fatty acids negatively correlated with fibrosis and inflammatory cell infiltration in CP. HR-MAS NMR may be used to analyze metabolic characteristics in a rat model of different degrees of chronic pancreatitis.

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Principal components analysis (A) scores plots and (B) loadings plots. From the data of 30 1H CPMG NMR spectra (δ0.50–4.60 ppm). n, control group; Δ, mild chronic pancreatitis group; and l, severe chronic pancreatitis group). CPMG, Carr-Purcell-Meiboom-Gill; NMR, nuclear magnetic resonance.
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f3-mmr-11-01-0053: Principal components analysis (A) scores plots and (B) loadings plots. From the data of 30 1H CPMG NMR spectra (δ0.50–4.60 ppm). n, control group; Δ, mild chronic pancreatitis group; and l, severe chronic pancreatitis group). CPMG, Carr-Purcell-Meiboom-Gill; NMR, nuclear magnetic resonance.

Mentions: Following the analysis of pancreatic tissue specimens obtained from the control, mild and severe CP groups by HR-MAS (Fig. 2), PCA was employed to screen 8 metabolic biochemicals including betaine, phosphocholine/glycerophosphocholine, choline, aspartate, lactate, fatty acids, 3-hydroxybutyrate and isoleucine/leucine/valine (Fig. 3), and the scores from each of the models within the three CP groups formed three distinct clusters (Fig. 3A).


Analysis of metabolic characteristics in a rat model of chronic pancreatitis using high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy.

Tian B, Ma C, Wang J, Pan CS, Yang GJ, Lu JP - Mol Med Rep (2014)

Principal components analysis (A) scores plots and (B) loadings plots. From the data of 30 1H CPMG NMR spectra (δ0.50–4.60 ppm). n, control group; Δ, mild chronic pancreatitis group; and l, severe chronic pancreatitis group). CPMG, Carr-Purcell-Meiboom-Gill; NMR, nuclear magnetic resonance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4237080&req=5

f3-mmr-11-01-0053: Principal components analysis (A) scores plots and (B) loadings plots. From the data of 30 1H CPMG NMR spectra (δ0.50–4.60 ppm). n, control group; Δ, mild chronic pancreatitis group; and l, severe chronic pancreatitis group). CPMG, Carr-Purcell-Meiboom-Gill; NMR, nuclear magnetic resonance.
Mentions: Following the analysis of pancreatic tissue specimens obtained from the control, mild and severe CP groups by HR-MAS (Fig. 2), PCA was employed to screen 8 metabolic biochemicals including betaine, phosphocholine/glycerophosphocholine, choline, aspartate, lactate, fatty acids, 3-hydroxybutyrate and isoleucine/leucine/valine (Fig. 3), and the scores from each of the models within the three CP groups formed three distinct clusters (Fig. 3A).

Bottom Line: The metabolite betaine positively correlated with fibrosis and inflammatory cell infiltration in CP.In addition, aspartate negatively correlated with fibrosis, but exhibited no significant correlation with inflammatory cell infiltration.Furthermore, the presence of fatty acids negatively correlated with fibrosis and inflammatory cell infiltration in CP.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Changhai Hospital of Shanghai, The Second Military Medical University, Shanghai 200433, P.R. China.

ABSTRACT
Pathological and metabolic alterations co-exist and co-develop in the progression of chronic pancreatitis (CP). The aim of the present study was to investigate the metabolic characteristics and disease severity of a rat model of CP in order to determine associations in the observed pathology and the metabolites of CP using high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR). Wistar rats (n=36) were randomly assigned into 6 groups (n=6 per group). CP was established by administering dibutyltin dichloride solution into the tail vein. After 0, 7, 14, 21, 28 and 35 days, the pancreatic tissues were collected for pathological scoring or for HR-MAS NMR. Correlation analyses between the major pathological scores and the integral areas of the major metabolites were determined. The most representative metabolites, aspartate, betaine and fatty acids, were identified as possessing the greatest discriminatory significance. The Spearman's rank correlation coefficients between the pathology and metabolites of the pancreatic tissues were as follows: Betaine and fibrosis, 0.454 (P=0.044); betaine and inflammatory cell infiltration, 0.716 (P=0.0001); aspartate and fibrosis, -0.768 (P=0.0001); aspartate and inflammatory cell infiltration, -0.394 (P=0.085); fatty acid and fibrosis, -0.764 (P=0.0001); and fatty acid and inflammatory cell infiltration, -0.619 (P=0.004). The metabolite betaine positively correlated with fibrosis and inflammatory cell infiltration in CP. In addition, aspartate negatively correlated with fibrosis, but exhibited no significant correlation with inflammatory cell infiltration. Furthermore, the presence of fatty acids negatively correlated with fibrosis and inflammatory cell infiltration in CP. HR-MAS NMR may be used to analyze metabolic characteristics in a rat model of different degrees of chronic pancreatitis.

Show MeSH
Related in: MedlinePlus